Leica Science Lab - Tag : Uncaging https://www.leica-microsystems.com//science-lab/tag/tags/uncaging/show/Tag/ Article tagged with Uncaging en-US https://www.leica-microsystems.com/19429 Fluorescence Microscopy What is Photomanipulation? The term photomanipulation describes a wide range of techniques that enable the microscopist the transition from passive observer to instigator of events by offering a way of interacting with their sample via targeted illumination. Typically researchers are trying to observe specific processes of interest in order to understand the underlying biological process. Microscopists are often forced to hunt through large populations of cells or acquire hours of time laps footage before they’re able to observe events of interest and in many cases it’s simply not possible to observe certain processes using conventional microscopy techniques alone. Photomanipulation tools enable the microscopist to initiate biological events, precisely adjusting sample labeling, biological activity, local chemical environments and in some instances physically destroy parts of their specimen. https://www.leica-microsystems.com//science-lab/what-is-photomanipulation/ Mon, 15 May 2017 07:59:00 +0000 Dr. Peter Laskey, Dr. Christoph Greb, Dr. Oliver Schlicker https://www.leica-microsystems.com/19348 Neuroscience Live-Cell Imaging Clap On, Clap Off. Protein On, Protein Off Unlike genetic or pharmacological manipulation, light-controlled proteins respond immediately, can be temporally and spatially triggered, are reversible, and are specific to the protein of interest. However, designing and using light-controllable proteins often requires expertise and specialized equipment. Now, two recently published articles in Science describe generalizable methods for making photo-controllable proteins. https://www.leica-microsystems.com//science-lab/clap-on-clap-off-protein-on-protein-off/ Tue, 02 May 2017 22:27:00 +0000