Leica Science Lab - Tag : Vesicle https://www.leica-microsystems.com//science-lab/tag/tags/vesicle/show/Tag/ Article tagged with Vesicle en-US https://www.leica-microsystems.com/18958 Super-Resolution Image Restoration and Deconvolution The Bif-1-Dynamin 2 Membrane Fission Machinery Regulates Atg9-Containing Vesicle Generation at the Rab11-Positive Reservoirs Application example of HyVolution Super-Resolution - Atg9 is a multispanning transmembrane protein that is required for autophagosome formation. During autophagy, vesicles containing Atg9 are generated through an unknown mechanism and delivered to the autophagosome formation sites. We have previously reported that Atg9-containing membranes undergo continuous tubulation and fission during nutrient starvation in a manner dependent on the curvature-inducing protein Bif-1/Sh3glb1. Here, we identify Dynamin 2 (DNM2) as a Bif-1-interacting protein that mediates the fission of Atg9-containing membranes during autophagy. https://www.leica-microsystems.com//science-lab/the-bif-1-dynamin-2-membrane-fission-machinery-regulates-atg9-containing-vesicle-generation-at-the-rab11-positive-reservoirs/ Wed, 25 Jan 2017 13:00:00 +0000 https://www.leica-microsystems.com/18905 Super-Resolution Neuroscience Botulinum Neurotoxin Type-A Enters a Non-Recycling Pool of Synaptic Vesicles Neuronal communication relies on synaptic vesicles undergoing regulated exocytosis and recycling for multiple rounds of fusion. Whether all synaptic vesicles have identical protein content has been challenged, suggesting that their recycling ability may differ greatly. Botulinum neurotoxin type-A (BoNT/A) is a highly potent neurotoxin that is internalized in synaptic vesicles at motor nerve terminals and induces flaccid paralysis. Recently, BoNT/A was also shown to undergo retrograde transport, suggesting it might enter a specific pool of synaptic vesicles with a retrograde trafficking fate. Using high-resolution microscopy techniques including electron microscopy and single molecule imaging, we found that the BoNT/A binding domain is internalized within a subset of vesicles that only partially co-localize with cholera toxin B-subunit and have markedly reduced VAMP2 immunoreactivity. https://www.leica-microsystems.com//science-lab/botulinum-neurotoxin-type-a-enters-a-non-recycling-pool-of-synaptic-vesicles/ Wed, 19 Oct 2016 11:51:00 +0000 https://www.leica-microsystems.com/16593 Super-Resolution Neuroscience Super-Resolution Microscopy of the Synaptic Active Zone At the presynaptic active zone (AZ) a variety of specialized proteins are assembled to complex architectures, which set the basis for speed, precision and plasticity of synaptic transmission. Recently, super-resolution microscopy (SRM) techniques have begun to enter the neurosciences. These approaches combine high spatial resolution with the molecular specificity of fluorescence microscopy. Here, we discuss how SRM techniques can be used to obtain information on the organization of AZ proteins. https://www.leica-microsystems.com//science-lab/super-resolution-microscopy-of-the-synaptic-active-zone/ Tue, 15 Dec 2015 10:15:00 +0000 Nadine Ehmann https://www.leica-microsystems.com/14658 Super-Resolution Neuroscience A New Probe for Super-Resolution Imaging of Membranes Elucidates Trafficking Pathways The molecular composition of the organelles involved in membrane recycling is difficult to establish as a result of the absence of suitable labeling tools. We introduce in this paper a novel probe, named membrane-binding fluorophore-cysteine-lysine-palmitoyl group (mCLING), which labels the plasma membrane and is taken up during endocytosis. https://www.leica-microsystems.com//science-lab/a-new-probe-for-super-resolution-imaging-of-membranes-elucidates-trafficking-pathways/ Fri, 17 Oct 2014 12:16:00 +0000 https://www.leica-microsystems.com/13877 Super-Resolution Neuroscience Super-Resolution Microscopy Helped to Create the First 3D Model of a Synapse A research team from Göttingen, led by Prof. Silvio O. Rizzoli, managed to determine the copy numbers and positions of all important building blocks of a synapse for the first time. This allowed them to reconstruct the first scientifically accurate 3D model of a synapse. https://www.leica-microsystems.com//science-lab/super-resolution-microscopy-helped-to-create-the-first-3d-model-of-a-synapse/ Tue, 01 Jul 2014 14:59:00 +0000 https://www.leica-microsystems.com/13740 Super-Resolution Neuroscience Interview with Dr. Yasushi Okada Yasushi Okada, team leader at Riken Quantitative Biology Center in Osaka, Japan, investigates vesicular transport mechanisms in neuronal cells. As the size of transported vesicles is below 100 nm and the diameter of microtubules is about 25 nm, he uses super-resolution techniques to study the sophisticated machinery of neuronal transport. https://www.leica-microsystems.com//science-lab/interview-with-dr-yasushi-okada/ Tue, 03 Jun 2014 15:38:00 +0000 Dipl. oec.-troph. Anja Schué, MD, PhD Yasushi Okada, PhD Isabelle Köster https://www.leica-microsystems.com/13373 Super-Resolution Neuroscience Synaptic Vesicle Recycling: Steps and Principles Synaptic vesicle recycling is one of the best‐studied cellular pathways. Many of the proteins involved are known, and their interactions are becoming increasingly clear. However, as for many other pathways, it is still difficult to understand synaptic vesicle recycling as a whole. https://www.leica-microsystems.com//science-lab/synaptic-vesicle-recycling-steps-and-principles/ Mon, 26 May 2014 09:30:00 +0000 Prof. Silvio Rizzoli https://www.leica-microsystems.com/13280 EM Sample Preparation Neuroscience Capturing Cellular Dynamics with Millisecond Temporal Resolution The combination of two powerful techniques: optogenetics and high-pressure freezing now makes it possible to visualize a dynamic cellular activity with temporal resolution of 5 milliseconds. By coupling a flash of light with high-pressure freezing, the process of vesicle recycling at the synapses can now be imaged by electron microscopy. https://www.leica-microsystems.com//science-lab/capturing-cellular-dynamics-with-millisecond-temporal-resolution/ Mon, 12 May 2014 13:16:00 +0000 PhD Shigeki Watanabe, PhD Erik M. Jørgensen https://www.leica-microsystems.com/11038 Confocal Microscopy Live-Cell Imaging Nobel Prize 2013 in Physiology or Medicine for Discoveries of the Machinery Regulating Vesicle Traffic On October 7th 2013, The Nobel Assembly at Karolinska Institutet has decided to award The Nobel Prize in Physiology or Medicine 2012 jointly to James E. Rothman, Randy W. Schekman and Thomas C. Südhof "for their discoveries of machinery regulating vesicle traffic, a major transport system in our cells". https://www.leica-microsystems.com//science-lab/nobel-prize-2013-in-physiology-or-medicine-for-discoveries-of-the-machinery-regulating-vesicle-traffic/ Fri, 11 Oct 2013 15:14:00 +0000 https://www.leica-microsystems.com/10214 Super-Resolution Neuroscience The Fate of Synaptic Vesicle Components upon Fusion Neurotransmitter release relies on the fusion of synaptic vesicles with the plasma membrane of synaptic boutons, which is followed by the recycling of vesicle components and formation of new vesicles. It is not yet clear whether upon fusion the vesicles persist as multimolecular patches in the plasma membrane, or whether they segregate into individual components. https://www.leica-microsystems.com//science-lab/the-fate-of-synaptic-vesicle-components-upon-fusion/ Fri, 01 Oct 2010 11:41:00 +0000 Dr. Felipe Opazo https://www.leica-microsystems.com/3006 Live-Cell Imaging TIRF Microscopy Exploring Cell Logistics Using TIRF microscopy, scientists have been able to take a closer look at intracellular transport processes with the example of the galactose-binding protein Galectin-3, which has been identified as a potential apical sorting receptor. https://www.leica-microsystems.com//science-lab/exploring-cell-logistics/ Fri, 02 Nov 2007 11:29:00 +0000 Prof. Ralf Jacob, Dipl. Biol. Dominik Schneider