Leica Science Lab - Tag : Widefield Microscopy https://www.leica-microsystems.com//science-lab/tag/tags/widefield-microscopy/show/Tag/ Article tagged with Widefield Microscopy en-US https://www.leica-microsystems.com/30136 Confocal Microscopy Fluorescence Microscopy Super-Resolution CLEM TIRF Microscopy Widefield Microscopy Live-Cell Imaging Laser Microdissection Microscopy in Virology The coronavirus SARS-CoV-2, causing the Covid-19 disease effects our world in all aspects. Research to find immunization and treatment methods, in other words to fight this virus, gained highest priority in 2020. Microscopes play a significant role in this kind of research. To understand the underlaying principles of receptor binding, genome release, replication, assembly, and virus budding, as well as the response of our immune system, different methodologies and microscopes can be used. This article summarizes why microscopy is an important tool in virology and infection biology and gives examples for different microscopy technologies and their applications in these research fields. https://www.leica-microsystems.com//science-lab/microscopy-in-virology/ Mon, 13 Jul 2020 12:13:00 +0000 Dr. Christoph Greb https://www.leica-microsystems.com/29672 Fluorescence Microscopy Widefield Microscopy Cholesterol Homeostasis Modulates Platinum Sensitivity in Human Ovarian Cancer Ovarian cancer is one of the most severe types of cancers that women can suffer from during their lifetimes. It is the cancer’s tendency for frequent relapses and drug resistance that leads to severity. High grade serous ovarian cancers are characterized by a special oxidative metabolism which has an influence on inflammation and drug resistance. TRAP1 is a chaperone which is involved in the regulation of mitochondrial respiration. https://www.leica-microsystems.com//science-lab/cholesterol-homeostasis-modulates-platinum-sensitivity-in-human-ovarian-cancer/ Tue, 02 Jun 2020 13:49:00 +0000 https://www.leica-microsystems.com/27942 Fluorescence Microscopy Widefield Microscopy Interview with Magali Mondin on THUNDER Imagers Magali Mondin, an engineer at the BIC (Bordeaux Imaging Center) in France, describes her experience using a THUNDER Imager in this interview. She had the opportunity to test extensively the THUNDER Imager 3D Tissue in her lab for several weeks on a wide variety of specimens. We met with her after the testing was finished to discuss the results she obtained and her experience working with the THUNDER technology. The BIC offers resources for photonic and electronic imaging, mainly in the life, health, and plant sciences. It is a core facility in France with a staff of 12 highly skilled engineers. The BIC has advanced bio-imaging techniques for fixed- and live-cell imaging, such as video microscopy, confocal microscopy, multiphoton microscopy, transmission electron microscopy, and scanning electron microscopy. https://www.leica-microsystems.com//science-lab/interview-with-magali-mondin-on-thunder-imagers/ Mon, 09 Mar 2020 12:43:00 +0000 Engineer Magali Mondin https://www.leica-microsystems.com/26925 Widefield Microscopy Drosophila Testis Niche Stem Cells – Three Color Computational Clearing Differentiated living beings such as humans, but also a fruit fly or a plant, possess not only the differentiated cells which form specific tissues, but also those cells whose fate is not yet (or only partially) fixed. Such cells are called stem cells. Stem cells serve to proliferate new cells for the specific tasks, but at the same time always produce stem cells, which in turn can supply new specific cells when needed later. https://www.leica-microsystems.com//science-lab/drosophila-testis-niche-stem-cells-three-color-computational-clearing/ Mon, 04 Nov 2019 11:42:00 +0000 Linda B. Morgan, Dr. Rolf T. Borlinghaus https://www.leica-microsystems.com/25096 Widefield Microscopy Alzheimer Plaques: fast Visualization in Thick Sections More than 60% of all diagnosed cases of dementia are attributed to Alzheimer’s disease. Typical of this disease are histological alterations in the brain tissue. So far, there is no cure for this disease. A few treatments try to slow down the fatal path or try to relieve the patients from symptoms. The laboratory of Dr. Mehrdad Shamloo at Stanford University studies pathological brain function with the goal of contributing to the discovery of novel therapeutics for Alzheimer’s disease. They use a mouse model of this disease to study the role of inflammation in Alzheimer’s disease progression. This requires imaging of thick uncleared brain tissues. https://www.leica-microsystems.com//science-lab/alzheimer-plaques-fast-visualization-in-thick-sections/ Thu, 27 Jun 2019 10:50:00 +0000 PhD Olga Davydenko, Dr. Rolf T. Borlinghaus https://www.leica-microsystems.com/24534 Widefield Microscopy How to Develop an Airway Epithelial Cell Model The present study describes the optimal growth conditions for the proliferation and differentiation of bovine bronchial epithelial cells (BBECs). The cells are grown at an air-liquid interface (ALI) in a serum-free medium to produce an airway epithelial cell (AEC) model. The AEC model accurately mimics the in vivo bovine respiratory epithelium. https://www.leica-microsystems.com//science-lab/how-to-develop-an-airway-epithelial-cell-model/ Wed, 06 Mar 2019 23:00:00 +0000 Eugenia Petoukhov https://www.leica-microsystems.com/24637 Widefield Microscopy Fluorescence Microscopy Live-Cell Imaging Stereo Microscopy THUNDER Technology Note So far, widefield microscopy was not suitable to image larger volumes, since the contrast of the recorded fluorescence image is reduced by the background (BG) mainly originating from out of focus regions of the recorded volume. BG reduces the effective dynamic range, the maximal possible signal to noise ratio (SNR), of the imaging system. https://www.leica-microsystems.com//science-lab/thunder-technology-note/ Tue, 05 Mar 2019 23:00:00 +0000 Dr. Jan Schumacher, Louise Bertrand https://www.leica-microsystems.com/20350 Widefield Microscopy Proline Metabolism is Essential for Trypanosoma brucei brucei Survival in the Tsetse Vector Bloodsucking insects play a major role in the transmission of pathogens that cause major tropical diseases. Their capacity to transmit these diseases is directly associated with the availability and turnover of energy sources. Proline is the main readily-mobilizable fuel of the tsetse fly, which is the vector of sub-species of Trypanosoma brucei parasites that cause human sleeping sickness and are partly responsible for animal trypanosomiasis (Nagana disease) in sub-Saharan Africa. https://www.leica-microsystems.com//science-lab/proline-metabolism-is-essential-for-trypanosoma-brucei-brucei-survival-in-the-tsetse-vector/ Sun, 29 Jul 2018 22:00:00 +0000 https://www.leica-microsystems.com/20175 Confocal Microscopy Widefield Microscopy mTORC1 Promotes Proliferation of Immature Schwann Cells and Myelin Growth of Differentiated Schwann Cells The myelination of axons is essential for neuronal wiring and normal nervous system functions. In the peripheral nervous system, Schwann cells (SCs) form myelin sheaths around axons during nerve development. Such myelination is compromised in a number of diseases. Hence, identification and understanding of the key pathways regulating SC development and myelinogenesis are essential for therapeutic progress. Here we uncover two separate roles of the cellular signaling node mTORC1 (mechanistic target of rapamycin complex 1) for regulating the development of SCs and subsequently the growth of myelin sheaths. Moreover, we demonstrate that defective SCs possess a remarkable plasticity to remyelinate axons via mTORC1. Thus, manipulating mTORC1 activity in diseased SCs could be therapeutically beneficial. https://www.leica-microsystems.com//science-lab/mtorc1-promotes-proliferation-of-immature-schwann-cells-and-myelin-growth-of-differentiated-schwann-cells/ Wed, 16 May 2018 22:00:00 +0000 https://www.leica-microsystems.com/19973 Widefield Microscopy Effect of curcumin analogs onα-synuclein aggregation and cytotoxicity Alpha-synuclein (α-Syn) aggregation into oligomers and fibrils is associated with dopaminergic neuron loss occurring in Parkinson’s disease (PD) pathogenesis. Compounds that modulate α-Syn aggregation and interact with preformed fibrils/oligomers and convert them to less toxic species could have promising applications in the drug development efforts against PD. Curcumin is one of the Asian food ingredient which showed promising role as therapeutic agent against many neurological disorders including PD. https://www.leica-microsystems.com//science-lab/effect-of-curcumin-analogs-ona-synuclein-aggregation-and-cytotoxicity/ Sun, 04 Mar 2018 23:00:00 +0000 https://www.leica-microsystems.com/19951 Widefield Microscopy Confocal Microscopy Expression Analysis of Platelet‐derived Growth Factor Receptor Alpha and its Ligands in the Developing Mouse Lung Activation of the platelet‐derived growth factor receptor‐α (PDGFR α) signaling pathway is critically important during lung alveogenesis, the process in lung development during which alveoli are formed from the terminal alveolar sacs. Several studies have aimed to characterize the expression patterns of PDGFR α and its two ligands (PDGF‐A and ‐C) in the lung, but published analyses have been limited to embryonic and/or perinatal time points, and no attempts have been made to characterize both receptor and ligand expression simultaneously. In this study, we present a detailed map of the expression patterns of PDGFR α, PDGF‐A and PDGF‐C during the entire period of lung development, that is, from early embryogenesis until adulthood. https://www.leica-microsystems.com//science-lab/expression-analysis-of-platelet-derived-growth-factor-receptor-alpha-and-its-ligands-in-the-developing-mouse-lung/ Tue, 20 Feb 2018 23:00:00 +0000 https://www.leica-microsystems.com/19922 Fluorescence Microscopy Widefield Microscopy Acute Transcriptional Up-regulation Specific to Osteoblasts/Osteoclasts in Medaka Fish Immediately after Exposure to Microgravity Bone loss is a serious problem in spaceflight; however, the initial action of microgravity has not been identified. To examine this action, we performed live-imaging of animals during a space mission followed by transcriptome analysis using medaka transgenic lines expressing osteoblast and osteoclastspecific promoter-driven GFP and DsRed. In live-imaging for osteoblasts, the intensity of osterix- or osteocalcin-DsRed fluorescence in pharyngeal bones was significantly enhanced 1 day after launch; and this enhancement continued for 8 or 5 days. https://www.leica-microsystems.com//science-lab/acute-transcriptional-up-regulation-specific-to-osteoblastsosteoclasts-in-medaka-fish-immediately-after-exposure-to-microgravity/ Tue, 13 Feb 2018 23:00:00 +0000 https://www.leica-microsystems.com/19812 Widefield Microscopy Live-Cell Imaging Adipose Stem Cells Display Higher Regenerative Capacities and More Adaptable Electro-Kinetic Properties Compared to Bone Marrow-Derived Mesenchymal Stromal Cells Adipose stem cells (ASCs) have recently emerged as a more viable source for clinical applications, compared to bone-marrow mesenchymal stromal cells (BM-MSCs) because of their abundance and easy access. In this study we evaluated the regenerative potency of ASCs compared to BM-MSCs. Furthermore, we compared the dielectric and electro-kinetic properties of both types of cells using a novel Dielectrophoresis (DEP) microfluidic platform based on a printed circuit board (PCB) technology. Our data show that ASCs were more effective than BM-MSCs in promoting neovascularization in an animal model of hind-limb ischemia. https://www.leica-microsystems.com//science-lab/adipose-stem-cells-display-higher-regenerative-capacities-and-more-adaptable-electro-kinetic-properties-compared-to-bone-marrow-derived-mesenchymal-stromal-cells/ Sun, 21 Jan 2018 23:00:00 +0000 https://www.leica-microsystems.com/19872 Super-Resolution Abstracts of the 7th European Super-Resolution User-Club Meeting The 7th Super-Resolution User Club Meeting was held in collaboration with Prof Pavel Hozák , at the Institute of Molecular Genetics of the ASCR in Prague. Keeping the event close to science is one of the founding principles of the event, allowing all participants to network, share and explore exciting new super-resolution and nanoscopy applications. Central to this are the scientific talks given during the meeting, with this cutting-edge microscopy technique as their central theme. A wide selection of topics were covered, prompting interesting discussions during the workshops. https://www.leica-microsystems.com//science-lab/abstracts-of-the-7th-european-super-resolution-user-club-meeting/ Sun, 17 Dec 2017 23:00:00 +0000 Prof. DrSc. Pavel Hozák, Prof. Dr. Torsten Ochsenreiter, Dr. Martin Offterdinger, Dr. Jordi Andilla, Ph.D. Marc van Zandvoort, Prof. Christian Eggeling, Dr. Susan Cox, Dr. Eugene Katrukha, Dr. Jindřiška Fišerová, Dr. Camille Boutin https://www.leica-microsystems.com/19548 Widefield Microscopy Basics in Microscopy Introduction to Widefield Microscopy One of the most basic microscopy techniques is known as ‘Widefield Microscopy’. It is fundamentally any technique in which the entire specimen of interest is exposed to the light source with the resulting image being viewed either by the observer or a camera (which can also be attached to a computer monitor). https://www.leica-microsystems.com//science-lab/introduction-to-widefield-microscopy/ Thu, 29 Jun 2017 11:31:00 +0000 PhD Martin Wilson https://www.leica-microsystems.com/18994 Fluorescence Microscopy Live-Cell Imaging Widefield Microscopy Chronic Inflammation Under the Microscope In the course of chronic inflammation certain body areas are recurrently inflamed. This goes along with many human diseases. With the help of widefield light microscopy, the underlying processes can be examined from a cellular level to whole organisms. This article presents several widefield microscopy applications such as immunofluorescence, live-cell imaging, histology, and ratiometric analysis to get insight into the development of chronic inflammation, the related diseases, and their treatment. https://www.leica-microsystems.com//science-lab/chronic-inflammation-under-the-microscope/ Mon, 09 Jan 2017 17:42:00 +0000 M.Sc. Jan Neumann, M.Sc. Anne Scherhag, Susanne Otten, Ph.D. Fangxia Shen, Anna Lena Leifke, Dr. Udo Birk, Dr. Christoph Greb, Dr. Kurt Lucas, Prof. Dr. Dr. Christoph Cremer https://www.leica-microsystems.com/17636 Widefield Microscopy Basics in Microscopy Infinity Optical Systems “Infinity Optics” refers to the concept of a beam path with parallel rays between the objective and the tube lens of a microscope. Flat optical components can be brought into this “Infinity Space” without influencing image formation, which is critical for the utilization of contrast methods such as DIC or fluorescence. Modern microscopy techniques require the addition of multiple optical instruments, such as light sources or laser devices, into the infinite light path. Different approaches to fulfill this need have emerged and are described here. https://www.leica-microsystems.com//science-lab/infinity-optical-systems/ Fri, 11 Mar 2016 16:28:00 +0000 Dr. Christoph Greb https://www.leica-microsystems.com/10555 Super-Resolution Three-Dimensional Super-Resolution GSDIM Microscopy With the new 3D GSDIM technique structures like the Golgi and the microtubular network are resolved not only laterally, but also in a third dimension. The principle is based on the use of optical astigmatism to determine the accurate lateral and axial position of individual fluorochromes. https://www.leica-microsystems.com//science-lab/three-dimensional-super-resolution-gsdim-microscopy/ Wed, 04 Sep 2013 15:24:00 +0000 PhD Tamara Straube, Sebastian Bänfer, Prof. Ralf Jacob https://www.leica-microsystems.com/6656 Live-Cell Imaging Quantitative Imaging Widefield Calcium Imaging with Calcium Indicator Fura2 In eukaryotic cells Ca2+ is one of the most widespread second messengers used in signal transduction pathways. Intracellular levels of Ca2+ are usually kept low, as Ca2+ often forms insoluble complexes with phosphorylated and carboxylated compounds. Typically cytosolic Ca2+ concentrations are in the range of 100 nM. In response to stimuli Ca2+ may either be released from external medium or internal stores to raise the Ca2+ concentration. https://www.leica-microsystems.com//science-lab/widefield-calcium-imaging-with-calcium-indicator-fura2/ Sun, 13 Jan 2013 23:00:00 +0000 Gabriele Burger, Simone Diehl https://www.leica-microsystems.com/6497 Widefield Microscopy Super-Resolution Widefield Super-Resolution with GSDIM Great advancements in biology have been possible by using fluorescence microscopy. So far, the resolution of the images was limited due to physical constraints. In the past couple of years, new methods evolved circumventing these limitations and bringing fluorescence microscopy to a new level of resolution, boosting the possibilities in science with fluorescence microscopes. https://www.leica-microsystems.com//science-lab/widefield-super-resolution-with-gsdim/ Wed, 11 Jul 2012 22:00:00 +0000 Dr. Marko Lampe https://www.leica-microsystems.com/6417 Widefield Microscopy Fluorescence Microscopy Live-Cell Imaging Image Processing for Widefield Microscopy Fluorescence microscopy is a modern and steadily evolving tool to bring light to current cell biological questions. With the help of fluorescent proteins or dyes it is possible to make discrete cellular components visible in a highly specific manner. A prerequisite for these kinds of investigations is a powerful fluorescence microscope. One special aim is the three-dimensional illustration of a structure to get an impression of full plasticity. This poses a certain problem for the experimenter using a classical light microscope. https://www.leica-microsystems.com//science-lab/deconvolution/ Wed, 04 Jul 2012 22:00:00 +0000 Dr. Christoph Greb https://www.leica-microsystems.com/4438 Super-Resolution Super-Resolution GSDIM Microscopy The nanoscopic technique GSDIM (ground state depletion microscopy followed by individual molecule return) provides a detailed image of the spatial arrangement of proteins and other biomolecules within the cell. There is now a first commercial system (the Leica SR GSD) on the market that is helping to make the GSDIM technique available to a wider group of users in research labs and imaging centers. https://www.leica-microsystems.com//science-lab/super-resolution-gsdim-microscopy/ Thu, 20 Oct 2011 22:00:00 +0000 PhD Tamara Straube, Alexandra Elli, Prof. Ralf Jacob https://www.leica-microsystems.com/3000 Live-Cell Imaging Neuroscience Mapping Billions of Synapses with Microscopy and Mathematics A combination of widefield imaging techniques and image segmentation analysis enable researchers to map learning-induced functional changes in individual synapses throughout the hippocampus. https://www.leica-microsystems.com//science-lab/mapping-billions-of-synapses-with-microscopy-and-mathematics/ Tue, 12 Apr 2011 22:00:00 +0000 Dr. Christopher S. Rex, Allison Paradise https://www.leica-microsystems.com/8082 Quantitative Imaging Widefield Microscopy FRET Sensitized Emission Wizard Widefield Förster Resonance Energy Transfer (FRET) is a technique, which allows insight into the interactions between proteins or molecules in proximities beyond light microscopic resolution. An excited fluorophore, called the donor, transfers its excited state energy to a light absorbing molecule which is called the acceptor. This transfer of energy is non-radiative. Sensitized Emission is one established method for the evaluation of FRET efficiencies. It can be applied to live cells as well as to fixed samples. https://www.leica-microsystems.com//science-lab/fret-sensitized-emission-wizard-widefield/ Tue, 14 Apr 2009 22:00:00 +0000 Markus Schechter, Gabriele Burger