Leica Science Lab - Tag : Widefield Microscopy https://www.leica-microsystems.com//science-lab/tag/tags/widefield-microscopy/show/Tag/ Article tagged with Widefield Microscopy en-US https://www.leica-microsystems.com/20175 Confocal Microscopy Widefield Microscopy mTORC1 Promotes Proliferation of Immature Schwann Cells and Myelin Growth of Differentiated Schwann Cells The myelination of axons is essential for neuronal wiring and normal nervous system functions. In the peripheral nervous system, Schwann cells (SCs) form myelin sheaths around axons during nerve development. Such myelination is compromised in a number of diseases. Hence, identification and understanding of the key pathways regulating SC development and myelinogenesis are essential for therapeutic progress. Here we uncover two separate roles of the cellular signaling node mTORC1 (mechanistic target of rapamycin complex 1) for regulating the development of SCs and subsequently the growth of myelin sheaths. Moreover, we demonstrate that defective SCs possess a remarkable plasticity to remyelinate axons via mTORC1. Thus, manipulating mTORC1 activity in diseased SCs could be therapeutically beneficial. https://www.leica-microsystems.com//science-lab/mtorc1-promotes-proliferation-of-immature-schwann-cells-and-myelin-growth-of-differentiated-schwann-cells/ Wed, 16 May 2018 22:00:00 +0000 https://www.leica-microsystems.com/19951 Widefield Microscopy Confocal Microscopy Expression Analysis of Platelet‐derived Growth Factor Receptor Alpha and its Ligands in the Developing Mouse Lung Activation of the platelet‐derived growth factor receptor‐α (PDGFR α) signaling pathway is critically important during lung alveogenesis, the process in lung development during which alveoli are formed from the terminal alveolar sacs. Several studies have aimed to characterize the expression patterns of PDGFR α and its two ligands (PDGF‐A and ‐C) in the lung, but published analyses have been limited to embryonic and/or perinatal time points, and no attempts have been made to characterize both receptor and ligand expression simultaneously. In this study, we present a detailed map of the expression patterns of PDGFR α, PDGF‐A and PDGF‐C during the entire period of lung development, that is, from early embryogenesis until adulthood. https://www.leica-microsystems.com//science-lab/expression-analysis-of-platelet-derived-growth-factor-receptor-alpha-and-its-ligands-in-the-developing-mouse-lung/ Tue, 20 Feb 2018 23:00:00 +0000 https://www.leica-microsystems.com/19872 Super-Resolution Abstracts of the 7th European Super-Resolution User-Club Meeting The 7th Super-Resolution User Club Meeting was held in collaboration with Prof Pavel Hozák , at the Institute of Molecular Genetics of the ASCR in Prague. Keeping the event close to science is one of the founding principles of the event, allowing all participants to network, share and explore exciting new super-resolution and nanoscopy applications. Central to this are the scientific talks given during the meeting, with this cutting-edge microscopy technique as their central theme. A wide selection of topics were covered, prompting interesting discussions during the workshops. https://www.leica-microsystems.com//science-lab/abstracts-of-the-7th-european-super-resolution-user-club-meeting/ Sun, 17 Dec 2017 23:00:00 +0000 Prof. DrSc. Pavel Hozák, Prof. Dr. Torsten Ochsenreiter, Dr. Martin Offterdinger, Dr. Jordi Andilla, Ph.D. Marc van Zandvoort, Prof. Christian Eggeling, Dr. Susan Cox, Dr. Eugene Katrukha, Dr. Jindřiška Fišerová, Dr. Camille Boutin https://www.leica-microsystems.com/19548 Widefield Microscopy Basics in Microscopy Image Restoration and Deconvolution Introduction to Widefield Microscopy One of the most basic microscopy techniques is known as ‘Widefield Microscopy’. It is fundamentally any technique in which the entire specimen of interest is exposed to the light source with the resulting image being viewed either by the observer or a camera (which can also be attached to a computer monitor). https://www.leica-microsystems.com//science-lab/introduction-to-widefield-microscopy/ Thu, 29 Jun 2017 11:31:00 +0000 PhD Martin Wilson https://www.leica-microsystems.com/18994 Fluorescence Microscopy Live-Cell Imaging Widefield Microscopy Image Restoration and Deconvolution Chronic Inflammation Under the Microscope In the course of chronic inflammation certain body areas are recurrently inflamed. This goes along with many human diseases. With the help of widefield light microscopy, the underlying processes can be examined from a cellular level to whole organisms. This article presents several widefield microscopy applications such as immunofluorescence, live-cell imaging, histology, and ratiometric analysis to get insight into the development of chronic inflammation, the related diseases, and their treatment. https://www.leica-microsystems.com//science-lab/chronic-inflammation-under-the-microscope/ Mon, 09 Jan 2017 17:42:00 +0000 M.Sc. Jan Neumann, M.Sc. Anne Scherhag, Susanne Otten, Ph.D. Fangxia Shen, Anna Lena Leifke, Dr. Udo Birk, Dr. Christoph Greb, Dr. Kurt Lucas, Prof. Dr. Dr. Christoph Cremer https://www.leica-microsystems.com/10555 Super-Resolution Three-Dimensional Super-Resolution GSDIM Microscopy With the new 3D GSDIM technique structures like the Golgi and the microtubular network are resolved not only laterally, but also in a third dimension. The principle is based on the use of optical astigmatism to determine the accurate lateral and axial position of individual fluorochromes. https://www.leica-microsystems.com//science-lab/three-dimensional-super-resolution-gsdim-microscopy/ Wed, 04 Sep 2013 15:24:00 +0000 PhD Tamara Straube, Sebastian Bänfer, Prof. Ralf Jacob https://www.leica-microsystems.com/10665 Super-Resolution Widefield Microscopy 3D Localization Microscopy With Ground State Depletion (GSD) With the latest development of a GSD 3D super-resolution platform, it is now possible to achieve a lateral resolution of down to 20 nm and an axial resolution of 70 nm. The technology is based on an astigmatism approach using a manipulated PSF to localize the molecule in z. This following tutorial describes the basic principles of the 3D GSD technology. https://www.leica-microsystems.com//science-lab/3d-localization-microscopy-with-ground-state-depletion-gsd/ Wed, 04 Sep 2013 12:47:00 +0000 PhD Tamara Straube https://www.leica-microsystems.com/6656 Live-Cell Imaging Quantitative Fluorescence Widefield Calcium Imaging with Calcium Indicator Fura2 In eukaryotic cells Ca2+ is one of the most widespread second messengers used in signal transduction pathways. Intracellular levels of Ca2+ are usually kept low, as Ca2+ often forms insoluble complexes with phosphorylated and carboxylated compounds. Typically cytosolic Ca2+ concentrations are in the range of 100 nM. In response to stimuli Ca2+ may either be released from external medium or internal stores to raise the Ca2+ concentration. https://www.leica-microsystems.com//science-lab/widefield-calcium-imaging-with-calcium-indicator-fura2/ Sun, 13 Jan 2013 23:00:00 +0000 Gabriele Burger, Simone Diehl https://www.leica-microsystems.com/6497 Widefield Microscopy Super-Resolution Widefield Super-Resolution with GSDIM Great advancements in biology have been possible by using fluorescence microscopy. So far, the resolution of the images was limited due to physical constraints. In the past couple of years, new methods evolved circumventing these limitations and bringing fluorescence microscopy to a new level of resolution, boosting the possibilities in science with fluorescence microscopes. https://www.leica-microsystems.com//science-lab/widefield-super-resolution-with-gsdim/ Wed, 11 Jul 2012 22:00:00 +0000 Dr. Marko Lampe https://www.leica-microsystems.com/6417 Widefield Microscopy Fluorescence Microscopy Live-Cell Imaging Image Restoration and Deconvolution Image Processing for Widefield Microscopy Fluorescence microscopy is a modern and steadily evolving tool to bring light to current cell biological questions. With the help of fluorescent proteins or dyes it is possible to make discrete cellular components visible in a highly specific manner. A prerequisite for these kinds of investigations is a powerful fluorescence microscope. One special aim is the three-dimensional illustration of a structure to get an impression of full plasticity. This poses a certain problem for the experimenter using a classical light microscope. https://www.leica-microsystems.com//science-lab/deconvolution/ Wed, 04 Jul 2012 22:00:00 +0000 Dr. Christoph Greb https://www.leica-microsystems.com/4621 Super-Resolution Widefield Microscopy How Widefield Super-Resolution GSDIM Images are Created The localization microscopy technique GSDIM is a proven technology to achieve super-resolution images with a resolution of up to 20 nm. In the following tutorial we will describe the basic principles and features of GSDIM. https://www.leica-microsystems.com//science-lab/how-widefield-super-resolution-gsdim-images-are-created/ Mon, 31 Oct 2011 17:17:00 +0000 Dr. Marko Lampe https://www.leica-microsystems.com/4600 Super-Resolution Step by Step Guide to the Molecular Basics of GSDIM Microscopy Ground state depletion microscopy followed by individual molecule return (GSDIM) is a super-resolution technique based on single molecule localization (Localization Microscopy). To localize single molecules and create a high resolution image the ensemble of overlapping fluorophores (in a diffraction-limited setup) has to be broken up. Individual fluorophores must be temporally "separated" to allow high precision detection of single molecules. This tutorial will explain the molecular basics of GSDIM. https://www.leica-microsystems.com//science-lab/step-by-step-guide-to-the-molecular-basics-of-gsdim-microscopy/ Fri, 28 Oct 2011 16:17:00 +0000 Dr. Marko Lampe https://www.leica-microsystems.com/4438 Super-Resolution Super-Resolution GSDIM Microscopy The nanoscopic technique GSDIM (ground state depletion microscopy followed by individual molecule return) provides a detailed image of the spatial arrangement of proteins and other biomolecules within the cell. There is now a first commercial system (the Leica SR GSD) on the market that is helping to make the GSDIM technique available to a wider group of users in research labs and imaging centers. https://www.leica-microsystems.com//science-lab/super-resolution-gsdim-microscopy/ Thu, 20 Oct 2011 22:00:00 +0000 PhD Tamara Straube, Alexandra Elli, Prof. Ralf Jacob https://www.leica-microsystems.com/3000 Live-Cell Imaging Neuroscience Image Restoration and Deconvolution Mapping Billions of Synapses with Microscopy and Mathematics A combination of widefield imaging techniques and image segmentation analysis enable researchers to map learning-induced functional changes in individual synapses throughout the hippocampus. https://www.leica-microsystems.com//science-lab/mapping-billions-of-synapses-with-microscopy-and-mathematics/ Tue, 12 Apr 2011 22:00:00 +0000 Dr. Christopher S. Rex, Allison Paradise https://www.leica-microsystems.com/8082 Quantitative Fluorescence Widefield Microscopy FRET Sensitized Emission Wizard Widefield Förster Resonance Energy Transfer (FRET) is a technique, which allows insight into the interactions between proteins or molecules in proximities beyond light microscopic resolution. An excited fluorophore, called the donor, transfers its excited state energy to a light absorbing molecule which is called the acceptor. This transfer of energy is non-radiative. Sensitized Emission is one established method for the evaluation of FRET efficiencies. It can be applied to live cells as well as to fixed samples. https://www.leica-microsystems.com//science-lab/fret-sensitized-emission-wizard-widefield/ Tue, 14 Apr 2009 22:00:00 +0000 Markus Schechter, Gabriele Burger