Leica Science Lab - Tag : Workflow https://www.leica-microsystems.com//science-lab/tag/tags/workflow/show/Tag/ Article tagged with Workflow en-US https://www.leica-microsystems.com/24965 Digital Microscopy Stereo Microscopy Quality Assurance How to create EDOF (Extended Depth of Focus) images Watch this video to see how you can rapidly record sharp optical microscope images of samples with a large height variation. This is done with the optional Extended Depth of Focus (EDOF) function of the LAS X software from Leica Microsystems. Acquisition of EDOF images of a printed circuit board, recorded from low to high magnification using a Leica microscope, are shown as an example. The operation of EDOF by acquiring a z-stack of images in 3 modes is demonstrated. https://www.leica-microsystems.com//science-lab/how-to-create-edof-extended-depth-of-focus-images/ Wed, 08 May 2019 22:00:00 +0000 Clinton Smith, Mike Horz https://www.leica-microsystems.com/24755 Live-Cell Imaging How can PAULA help streamline the workflow for my wound healing assay? The wound healing assay, often used as a synonym for scratch assay or migration assay, is an important tool for the evaluation and measurement of cell migration, whether single cells or cell populations. Cell migration is tightly controlled during ontogenetic processes and at times can get out of control, e.g., during cancer development and progression. https://www.leica-microsystems.com//science-lab/how-can-paula-help-streamline-the-workflow-for-my-wound-healing-assay/ Mon, 18 Mar 2019 23:00:00 +0000 Dr. Karin Schwab https://www.leica-microsystems.com/24702 EM Sample Preparation Webinar: Integrated Cryo-tomography Workflow Analyzing purified protein structures in a cellular context is key to understanding how proteins and protein complexes function. Thermo Fisher Scientific and Leica Microsystems developed the integrated cryo-tomography workflow to enable high resolution structural analysis, done in a controlled and effective way, of proteins in their native environments. In this webinar, the speakers will explain the unique features of the hardware and software integration and guide you step-by-step through the workflow. https://www.leica-microsystems.com//science-lab/changing-the-way-we-look-at-the-inner-workings-of-cells/ Sun, 03 Mar 2019 23:00:00 +0000 https://www.leica-microsystems.com/24680 Live-Cell Imaging Confluency Check with PAULA Cell Imager Many cell-based experiments require cells in a certain state. This can include their morphology,  fluorescent protein expression, and the confluency of the cell layer. Researchers check their cells regularly to determine these attributes. Confluency estimation can be very difficult! This guess work is biased by every individual researcher, and can result in lab error.  https://www.leica-microsystems.com//science-lab/confluency-check-with-paula-cell-imager/ Wed, 20 Feb 2019 23:00:00 +0000 Dr. Christoph Greb https://www.leica-microsystems.com/20653 Laser Microdissection Webinar: Live Cell Isolation by Laser Microdissection Laser microdissection is a tool for the isolation of homogenous cell populations from their native niches in tissues to downstream molecular assays. Beside its routine use for fixed tissue sections, laser microdissection may be applied for live cell isolation. Unlike other well-established and widely used techniques for live cell isolation and single cell cloning—such as FACS, MACS, cloning by limited dilution, and so on—laser microdissection allows for capturing live cells and cell colonies without their detachment from the carrier. https://www.leica-microsystems.com//science-lab/webinar-live-cell-isolation-by-laser-microdissection/ Tue, 16 Oct 2018 22:00:00 +0000 https://www.leica-microsystems.com/20212 EM Sample Preparation Metallography Quality Assurance Stereo Microscopy 3-Dimensional Imaging of Macroscopic Defects in Aluminum Alloys The investigation of macroscale defects in aluminum (Al) alloys with a rapid 3-dimensional (3D) imaging approach is described in this report. Aluminum (Al) alloys play an important role in the production of aircraft and vehicles, as well as products in other industries. Defects present in the Al alloy used for the production of aircraft, vehicles, or other products can have a significant effect on their quality, performance, and lifetime. https://www.leica-microsystems.com//science-lab/3-dimensional-imaging-of-macroscopic-defects-in-aluminum-alloys/ Tue, 12 Jun 2018 22:00:00 +0000 PhD Wolfgang Grünewald, PhD James DeRose https://www.leica-microsystems.com/19763 Metallography Quality Assurance Forensics See the Structure with Microscopy - Know the Composition with Laser Spectroscopy The advantages of a 2-in-1 materials analysis solution combining optical microscopy and laser induced breakdown spectroscopy (LIBS) for simultaneous visual and chemical inspection are described in this report. The basic principles of the 2-in-1 solution and a comparison between it and other common materials analysis methods, such scanning electron microscopy (SEM), are explained to demonstrate how a rapid, efficient workflow is achieved. A 2-in-1 analysis solution can reduce significantly the cost and time for obtaining material image and composition data. Such data are instrumental in assuring quality and reliability to make confident decisions quickly during production, quality control, failure analysis, and research and development in industries and fields, such as automotive and metallurgy. https://www.leica-microsystems.com//science-lab/see-the-structure-with-microscopy-know-the-composition-with-laser-spectroscopy/ Mon, 11 Jun 2018 13:39:00 +0000 PhD James DeRose, Dr. Kay Scheffler https://www.leica-microsystems.com/20117 Metallography Leica is MAD about LIBS When analyzing material for cleanliness testing, users normally just want a simple way to know whether the particle under scrutiny is normal debris or something more risky. Users can now “LIBS” it to acquire rapidly the composition and then move onto the next manufacturing step. https://www.leica-microsystems.com//science-lab/leica-is-mad-about-libs/ Sun, 22 Apr 2018 22:00:00 +0000 Mike Tjepkema, PhD James DeRose https://www.leica-microsystems.com/19946 Metallography Quality Assurance Rate the Quality of Your Steel: Free Webinar and Report This webinar and report describe optimal microscopy solutions for rating steel quality in terms of non-metallic inclusions and reviews the various international and regional standards concerning rigorous quality assessment methods, e.g., EN 10247, ASTM E45, DIN 50602, and ISO 4967. https://www.leica-microsystems.com//science-lab/rating-the-quality-of-steel/ Thu, 15 Feb 2018 23:00:00 +0000 Dionis Diez, PhD James DeRose, Thomas Locherer https://www.leica-microsystems.com/19216 Ergonomics Stereo Microscopy How to Turn Microscope Workplaces Ergonomic Microscopes are tools that affect those who work with them every day. They can be highly demanding for the human body, requiring concentration and a lot of steady activity from many of our muscles. In this interview, Clinton Smith, Senior Product Manager at Leica Microsystems, talks about how to relieve possible tension and strain and how to create ergonomic workplaces to help microscope users work in comfort and how to increase productivity. https://www.leica-microsystems.com//science-lab/ergonomics/how-to-turn-microscope-workplaces-ergonomic/ Thu, 09 Feb 2017 13:08:00 +0000 Claudia Müller https://www.leica-microsystems.com/18999 Digital Microscopy Quality Assurance How Digital Microscopy can Contribute to Efficient Workflows for Microelectronics and Electronics This report explains how users can benefit from the digital microscope portfolio of Leica Microsystems to attain cost-effectiveness over entire workflows in research and development (R&D), product innovation, process engineering, production, quality control and assurance (QC/QA), and failure analysis (FA). https://www.leica-microsystems.com//science-lab/how-digital-microscopy-can-contribute-to-efficient-workflows-for-microelectronics-and-electronics/ Wed, 30 Nov 2016 12:36:00 +0000 PhD James DeRose, Oscar Rodriguez https://www.leica-microsystems.com/17540 Stereo Microscopy Live-Cell Imaging Fluorescence Microscopy Work More Efficiently in Developmental Biology With Stereo Microscopy: Zebrafish, Medaka, and Xenopus Among the aquatic model organisms used in molecular and developmental biology the most prominent are the zebrafish (genus species: Danio rerio), medaka or japanese rice fish (genus species: Oryzias latipes), and african clawed frog (genus species: Xenopus laevis). This report gives useful information to scientists and technicians which can help improve their daily laboratory work by making the steps of transgenesis, fluorescent screening, and functional imaging more efficient. https://www.leica-microsystems.com//science-lab/work-more-efficiently-in-developmental-biology-with-stereo-microscopy-zebrafish-medaka-and-xenopus/ Thu, 27 Oct 2016 07:50:00 +0000 PhD James DeRose, Anastasia Felker, Dr. Elena Chiavacci, Gianluca D’Agati, Ph.D. Christian Mosimann, Ph.D. Jens Peter Gabriel, PhD Heinrich Bürgers, Ph.D. Adam Cliffe https://www.leica-microsystems.com/18806 Super-Resolution Practical Guide for Excellent GSDIM Super-Resolution Images Do you know that most protists and bacteria lack in one feature that each of our body cell has? Our cells are touch and communicate with one another. They send and receive a variety of signals that coordinate their behavior to act together as a functional multicellular organism. Exploring the way of cellular communication and the ways how the cell surface interacts to organize tissues and body structures is of great interest. Kees Jalink and his team of scientists at the Netherlands Cancer Institute (NKI) in Amsterdam obtained new scientific insights into the molecular architecture of hemidesmosomes, cytoskeletal components, cell surface receptors and vesicular proteins with the help of Ground-State-Depletion (GSD)/ dSTORM microscopy. In this interview, Kees Jalink comments on their developments in imaging chambers, buffer conditions and image analysis to get the perfect super resolution image. https://www.leica-microsystems.com//science-lab/practical-guide-for-excellent-gsdim-super-resolution-images/ Wed, 26 Oct 2016 06:58:00 +0000 PhD Kees Jalink, PhD Tamara Straube, MSc Leila Nahidiazar, MSc Daniela Leyton Puig https://www.leica-microsystems.com/17538 Forensics Digital Microscopy Forensics: How to Gather Evidence from Ballistics, Tool Marks, Questioned Documents, Counterfeiting, and Forgery Fast and Easily with Digital Microscopy The examples reported here demonstrate how digital microscopy enables forensic scientists to gather and compare evidence from ballistics and tool marks or counterfeited money, credit cards, and documents, more efficiently. Images and videos clearly show how to use the digital microscope to perform forensics investigations and how you speed up your workflow with the help of practical features, such as a convenient way to change magnification rapidly over the full range, tilting, sample rotation, and versatile illumination. https://www.leica-microsystems.com//science-lab/forensics/forensics-how-to-gather-evidence-from-ballistics-tool-marks-questioned-documents-counterfeiting-and-forgery-fast-and-easily-with-digital-microscopy/ Fri, 07 Oct 2016 14:19:00 +0000 PhD James DeRose, MSc Michael Doppler https://www.leica-microsystems.com/18691 Stereo Microscopy Confocal Microscopy Fluorescence Microscopy Work Efficiently in Developmental Biology with Stereo and Confocal Microscopy: C. elegans For scientists, technicians, and teachers working with the worm C. elegans in the research lab or classroom, this report is intended to give useful information to help improve their daly work. The aim is to make the work steps of worm picking, transgenesis, RNA interference, screening, and functional imaging efficient. It also details the various possibilities for equipping a research worm lab or biology classroom/teaching lab explaining worm methods. https://www.leica-microsystems.com//science-lab/work-efficiently-in-developmental-biology-with-stereo-and-confocal-microscopy-c-elegans/ Mon, 19 Sep 2016 06:09:00 +0000 PhD James DeRose, PhD Heinrich Bürgers, PhD Martin Gamerdinger https://www.leica-microsystems.com/17941 Laser Microdissection Workflows & Protocols: Plant Laser Microdissection During Leica workshops for LMD users in Brazil, hosted by the Federal University of Paraná/UFPR (UFPR) at the Centro de Energia Nuclear na Agricultura/USP (CENA), the power of laser microdissection using the Leica LMD systems was demonstrated. One special focus was on plant dissection which needs a high laser power. https://www.leica-microsystems.com//science-lab/workflows-protocols-plant-laser-microdissection/ Mon, 06 Jun 2016 05:22:00 +0000 Dr. Falk Schlaudraff, Dr. Christoph Greb https://www.leica-microsystems.com/17943 Quality Assurance Digital Microscopy Fast and Reliable Inspection of Printed Circuit Boards with Digital Microscopy Digital microscopy has been used more and more for inspection, quality control and assurance (QC/QA), failure analysis (FA), and research and development (R&D) in the microelectronics industry, especially for printed circuit boards (PCBs). Digital microscopes are practical to use and allow an efficient workflow for inspection. https://www.leica-microsystems.com//science-lab/fast-and-reliable-inspection-of-printed-circuit-boards-with-digital-microscopy/ Mon, 23 May 2016 12:26:00 +0000 PhD James DeRose, Giancarlo Parma https://www.leica-microsystems.com/17939 Laser Microdissection Workflows & Protocols: Laser Microdissection for Pathology and Cancer Research Tumor development results from mutations in our DNA. For their deeper analysis, cancer researchers have to dissect the relevant tissue areas. Here we report the reason why laser microdissection is a perfect tool for this purpose and how this was taught in the course of a workshop held in Brazil. With the Leica LMD system pure tumor material can be selected and dissected for downstream analysis to ensure 100% pure starting material without any risk of cross contamination with healthy cells. https://www.leica-microsystems.com//science-lab/workflows-protocols-laser-microdissection-for-pathology-and-cancer-research/ Wed, 04 May 2016 11:30:00 +0000 Dr. Falk Schlaudraff, Dr. Christoph Greb https://www.leica-microsystems.com/17665 Quality Assurance Digital Microscopy Automotive Industry: How Suppliers and Auto Manufacturers Can Verify Parts Specifications Quickly and Easily Checking specifications is critical: During the manufacture of auto parts, specifications must be met – whether by the auto parts supplier or the automobile manufacturer. It is important that the parts meet specifications as they are critical for maintaining the performance standards and safe operation of automobiles, trucks, and other vehicles during their lifetimes. https://www.leica-microsystems.com//science-lab/automotive-industry-how-suppliers-and-auto-manufacturers-can-verify-parts-specifications-quickly-and-easily/ Mon, 25 Apr 2016 08:21:00 +0000 PhD James DeRose, André Reinhold, Georg Schlaffer https://www.leica-microsystems.com/17667 Live-Cell Imaging Widefield Microscopy How to do a Proper Cell Culture Quick Check In order to successfully work with mammalian cell lines, they must be grown under controlled conditions and require their own specific growth medium. In addition, to guarantee consistency their growth must be monitored at regular intervals. This article describes a typical workflow for subculturing an adherent cell line with detailed illustrations of all of the necessary steps. https://www.leica-microsystems.com//science-lab/how-to-do-a-proper-cell-culture-quick-check/ Thu, 24 Mar 2016 09:18:00 +0000 PhD Tamara Straube, Claudia Müller https://www.leica-microsystems.com/17398 Quality Assurance Digital Microscopy Inspecting and Analyzing Printed Circuit Boards Quickly and Reliably with a Digital Microscope For the past several years, digital microscopy has been shown to be useful for inspection, quality control and assurance (QC/QA), and failure analysis (FA) in the microelectronics industry, especially for printed circuit boards (PCBs). Recently, state-of-the-art improvements have made digital microscopy even more powerful and practical for inspection, leading to a more efficient workflow. Here, the advantages of certain digital microscope features, i.e., intuitive software for operation and analysis, fast and easy ways to change magnification, and encoding for reliable recall of parameters, are explained. https://www.leica-microsystems.com//science-lab/inspecting-and-analyzing-printed-circuit-boards-quickly-and-reliably-with-a-digital-microscope/ Mon, 11 Jan 2016 09:09:00 +0000 PhD James DeRose, Georg Schlaffer https://www.leica-microsystems.com/17370 Quality Assurance Digital Microscopy Automotive Industry: Rapid and Precise Surface Inspection on Hard-to-Image Samples In the automotive industry, microscopists may be challenged with samples that are difficult to image, for example, parts that have a special material composition. This report shows how the Leica DVM6 digital microscope helps to make inspection, measurement analysis, and reporting of such challenging samples quicker and easier. https://www.leica-microsystems.com//science-lab/automotive-industry-rapid-and-precise-surface-inspection-on-hard-to-image-samples/ Mon, 04 Jan 2016 09:58:00 +0000 PhD James DeRose, Georg Schlaffer https://www.leica-microsystems.com/16814 Laser Microdissection Neuroscience Workflows & Protocols: Connecting Microscopy and Molecular Biology in Neuroscience The main topic during this course was how to apply laser microdissection in neuroscience. Leica specialists demonstrated why laser microdissection is a suitable techniques for brain investigation as it allows to separate distinct brain layers or even to isolate individual neurons. https://www.leica-microsystems.com//science-lab/workflows-protocols-connecting-microscopy-and-molecular-biology-in-neuroscience/ Fri, 04 Dec 2015 16:01:00 +0000 Dr. Falk Schlaudraff https://www.leica-microsystems.com/16808 Laser Microdissection Workflows & Protocols: How to Isolate Individual Chromosomes with Laser Microdissection During the first Leica Workshop in Brazil, at the Centro de Energia Nuclear na Agricultura/USP (CENA), the participants learned how to prepare samples for laser microdissection (LMD) using a cryotome. Another topic was the dissection of individual chromosomes from chromosome spreads. Leica specialists held a short training session with the LMD. After this, new LMD users were able to run the system and practiced how to dissect chromosomes and collect single chromosomes for downstream analysis. https://www.leica-microsystems.com//science-lab/workflows-protocols-how-to-isolate-individual-chromosomes-with-laser-microdissection/ Thu, 03 Dec 2015 20:06:00 +0000 Dr. Falk Schlaudraff https://www.leica-microsystems.com/16110 Laser Microdissection Workflows & Protocols: How to Use a Leica Laser Microdissection System and Qiagen Kits for Successful RNA Analysis Laser Microdissection (LMD) allows isolating individual cells or chromosomes and is a well established technique for sample preparation prior downstream analysis of the nucleic acid content via PCR or sequencing techniques. Here we describe the successful combination of the Leica Microsystems LMD system and Qiagen kits for purification of nucleic acids even from little amounts. https://www.leica-microsystems.com//science-lab/workflows-protocols-how-to-use-a-leica-laser-microdissection-system-and-qiagen-kits-for-successful-rna-analysis/ Fri, 10 Jul 2015 14:26:00 +0000 Dr. Falk Schlaudraff https://www.leica-microsystems.com/15767 Digital Microscopy Quality Assurance What You Always Wanted to Know About Digital Microscopy, but Never Got Around to Asking Digital microscopy is one of the buzz words in microscopy – and there are a couple of facts that are useful to know. Georg Schlaffer, Product Manager with Leica Microsystems, has often been asked about digital microscopy by customers and colleagues alike. He has worked together with Scientific Writer Jim DeRose for comprehensive answers to the most important ones. https://www.leica-microsystems.com//science-lab/what-you-always-wanted-to-know-about-digital-microscopy-but-never-got-around-to-asking/ Wed, 27 May 2015 11:40:00 +0000 PhD James DeRose, Georg Schlaffer https://www.leica-microsystems.com/15287 Quality Assurance Metallography Five Advantages of Inverted Over Upright Microscopes in Industrial Applications With inverted microscopes, you look at samples from below since their optics are placed under the sample, with upright microscopes you look at samples from above. Traditionally, inverted microscopes are used for life science research, because gravity makes samples sink to the bottom of a holder with aqueous solution and you don’t see a lot from above. https://www.leica-microsystems.com//science-lab/five-advantages-of-inverted-over-upright-microscopes-in-industrial-applications/ Mon, 16 Mar 2015 09:06:00 +0000 Dr. Kay Scheffler, Claudia Müller https://www.leica-microsystems.com/15992 Digital Microscopy Quality Assurance Are Your Digital Microscope Measurements Accurate and Reliable? For certain applications, such as the production and maintenance of automobiles, aircraft, or power plants, quality control and reliability assurance, as well as safety and health inspection, accurate and reliable image data with precise calibration are very important. Digital microscopes are appealing for a wide range of technical applications in various industries, such as automotive, aerospace, precision engineering, microelectronics, and medical devices. https://www.leica-microsystems.com//science-lab/are-your-digital-microscope-measurements-accurate-and-reliable/ Mon, 08 Sep 2014 11:01:00 +0000 PhD James DeRose, Georg Schlaffer https://www.leica-microsystems.com/10864 Ergonomics Stereo Microscopy Quality Assurance Higher Motivation, Longer Concentration – Ergonomics as a Competitive Advantage Sensor and identification technology, industrial cameras: when top precision is required, the name Baumer Electric is soon mentioned. The Swiss company designs and produces components for manufacturing facilities all over the world. To be able to guarantee that the finished components are of the highest possible quality, elaborate quality inspections are carried out part for part under the stereo microscope. https://www.leica-microsystems.com//science-lab/higher-motivation-longer-concentration-ergonomics-as-a-competitive-advantage/ Tue, 01 Oct 2013 09:38:00 +0000 Michael Birlenbach, Remo Holenstein https://www.leica-microsystems.com/9449 Surgical Microscopy Ergonomics Ergonomically Designed Surgical Microscopes Support Performance Operations not only demand maximum concentration of the surgeon but also maximum physical fitness. Physiotherapist John Ludescher explains how the body can be given optimal support to perform at its best. The Austrian qualified as physiotherapist in Switzerland and has had his own practice for nine years. He is the author of the book “Aufrecht – Bewusst – Stark” (Upright – Aware – Strong) and designer of the “PhysioCap”. https://www.leica-microsystems.com//science-lab/ergonomically-designed-surgical-microscopes-support-performance/ Mon, 01 Jul 2013 17:41:00 +0000 Kerstin Pingel, John Ludescher https://www.leica-microsystems.com/7855 Quantitative Fluorescence Confocal Microscopy Live-Cell Imaging Fluorescence Microscopy Integrative Open-Source Software for Image Analysis in Biology Imaging techniques are indispensable in many fields of life sciences today. With state-of-the-art optics and metrology, they provide hundreds of gigabytes of still images and videos. Correspondingly, there is a growing need for complex software solutions to ensure that the amounts of generated data can be automatically managed, processed and analyzed – and shared online with a large group of users. The combination of individual open-source software projects is proving especially useful for solving such complex image analysis problems. https://www.leica-microsystems.com//science-lab/integrative-open-source-software-for-image-analysis-in-biology/ Tue, 27 Nov 2012 23:00:00 +0000 Christian Dietz, Martin Horn, Prof. Dr. Michael Berthold https://www.leica-microsystems.com/7793 Ergonomics Stereo Microscopy Body in Balance Nobody is the same. Whether large or small, well- or slightly built, left- or right-handed, everyone has different demands of the tool they work with for many hours a day. This is particularly true of laboratory workstations, where routine tasks such as pipetting or microscope or microtome work require a static posture. https://www.leica-microsystems.com//science-lab/body-in-balance/ Thu, 15 Nov 2012 23:00:00 +0000 Kerstin Pingel, John Ludescher