Leica Science Lab - Tag : Working Distance https://www.leica-microsystems.com//science-lab/tag/tags/working-distance/show/Tag/ Article tagged with Working Distance en-US https://www.leica-microsystems.com/19673 Basics in Microscopy Immersion Objectives: Using Oil, Glycerol, or Water to Overcome some of the Limits of Resolution To examine specimens at high magnifications using the microscope, there are a number of factors which need to be taken into consideration. These include resolution, numerical aperture (NA), the working distance of objectives and the refractive index of the medium through which the image is collected by the front lens of an objective. In this article, we will briefly look at how using an immersion medium between the coverslip and the objective front lens helps to increase the NA and resolution. https://www.leica-microsystems.com//science-lab/immersion-objectives-using-oil-glycerol-or-water-to-overcome-some-of-the-limits-of-resolution/ Wed, 09 Aug 2017 12:07:00 +0000 PhD Martin Wilson https://www.leica-microsystems.com/18779 Multiphoton Microscopy BABB Clearing and Imaging for High Resolution Confocal Microscopy: Counting and Sizing Kidney Cells in the 21st Century Multipohoton microscopy experiment using Leica TCS SP8 MP and Leica 20x/0.95 NA BABB immersion objective. Understanding kidney microanatomy is key to detecting and identifying early events in kidney disease. Improvements in tissue clearing and imaging have been crucial in this field, and now we report on a novel, time-efficient method to study podocyte depletion in renal glomeruli using a combination of immunofluorescence, optical clearing, confocal microscopy and 3D analysis. https://www.leica-microsystems.com//science-lab/babb-clearing-and-imaging-for-high-resolution-confocal-microscopy-counting-and-sizing-kidney-cells-in-the-21st-century/ Tue, 13 Sep 2016 09:03:00 +0000 Ph.D. Victor G. Puelles, Prof. John F. Bertram, MSc Stephen Firth, Prof. Ian Harper https://www.leica-microsystems.com/17444 Digital Microscopy Image gallery: Life Science Imaging with the Leica DVM6 Digital Microscope Digital microscopes can be a great help in life science applications such as the documentation in botany, entomology studies and crop science, or the digitization of museum collections. The image quality digital microscopes yield, is stunning – this image gallery gives you an idea. Enjoy! https://www.leica-microsystems.com//science-lab/galleries/image-gallery-digital-microscopy/image-gallery-life-science-imaging-with-the-leica-dvm6-digital-microscope/ Mon, 22 Feb 2016 15:42:00 +0000 PhD James DeRose, PhD Heinrich Bürgers https://www.leica-microsystems.com/5098 Stereo Microscopy Ergonomics Basics in Microscopy Quality Assurance Factors to Consider When Selecting a Stereo Microscope Stereo microscopes are often nicknamed the workhorse of the lab or the production department. Users spend many hours behind the ocular inspecting, observing, documenting or dissecting samples. Which factors need to be considered when selecting... https://www.leica-microsystems.com//science-lab/factors-to-consider-when-selecting-a-stereo-microscope/ Thu, 26 Jan 2012 23:00:00 +0000 Daniel Goeggel