Fixation and Dehydration:
2% Paraformaldehyde, 2.5% Glutaraldehyde, 0.1M Cacodylate Buffer, pH 7.3 | 2 h |
0.1M Sodium Cacodylate Buffer, pH 7.3 | 2x10 min. |
1% aqueous OsO4 | 1-2 h |
Destilled water | 3x10 min. |
Ethanol series: 50%, 70%, 95%, 100% | 3x10 min. |
Leica EM CPD300 auto Program:


Coating:
Platinum: 15-20 nm