Species:
Tobacco (Nicotiana tabacum)
Critical point drying of tobacco leafs with subsequent platinum coating and SEM analysis.
Procedure:
Sample Holder: Samples were placed into the filter discs and porous pots holder.
Fixation and Dehydration:
| 2% Paraformaldehyde, 2.5% Glutaraldehyde, 0.1M Cacodylate Buffer, pH 7.3 | 2 h |
| 0.1M Sodium Cacodylate Buffer, pH 7.3 | 2x10 min. |
| 1% aqueous OsO4 | 1-2 h |
| Destilled water | 3x10 min. |
| Ethanol series: 50%, 70%, 95%, 100% | 3x10 min. |
Leica EM CPD300 auto Program:
Coating:
Platinum: 15-20 nm
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