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Camera Technology

Digital cameras play a vital role in modern microscope systems. Nowadays digital cameras have even begun to replace oculars in some digital microscopesMicroscope cameras serve users across a broad range of application fields and capture the data that form the basis of further analyses. The microscope optics determine the formation of the image which is then projected onto the camera sensor. So the optics are responsible for the quality of the image that reaches the sensor, while the camera is responsible for the accurate digitization and documentation. The camera must be carefully matched to the microscope and application to ensure the image is accurately captured by the sensor, be it for brightfield or fluorescence imaging.

  • Definitions of Basic Technical Terms for Digital Microscope Cameras and Image Analysis

    Most microscopes today are operated with a camera. The characteristics of the camera often decide whether the acquired image will reveal what a researcher wants to see. But when diving into camera terminology, the technical terms can be overwhelming. We have compiled the most important terms with a concise explanation to provide orientation.
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  • What Makes sCMOS Microscope Cameras so Popular?

    sCMOS cameras are more sensitive and are capable of much higher acquisition speed than cameras with other sensor types. Even though CCD cameras are widely used in live cell imaging and time-lapse recordings, researchers are often concerned that their camera does not detect faint signals. In this interview, Dr. Karin Schwab, Product Manager at Leica Microsystems, talks about the characteristics of sCMOS cameras and how researchers benefit from the latest camera sensor technology.
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  • Introduction to Digital Camera Technology

    A significant majority of modern optical microscopy techniques require the use of a digital camera. By working with digital devices researchers can observe specimens on a screen in real time or acquire and store images and quantifiable data. Here we introduce the basic principles behind digital camera technologies commonly encountered in scientific imaging.
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  • Towards Digital Photon Counting Cameras for Single-molecule Optical Nanoscopy

    A SPAD array camera with single-photon sensitivity and zero read-out noise allows for the detection of extremely weak signals at ultra-fast imaging speeds. With temporal resolution in the order of micro-seconds, a SPAD array camera offers great potential for live-cell imaging with super-resolution.
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  • Digital Camera Technologies for Scientific Bio-Imaging

    This four-part series of articles published in Microscopy and Analysis covers the factors to consider in choosing a camera among CCD, EMCCD, and scientific-grade CMOS camera technologies for biological imaging applications. The differences among the sensor architectures and the impact of parameters such as pixel size, noise, and QE on signal-to-noise performance, image quality, and Nyquist sampling are considered.
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  • Digital Cameras

    Manufacturers digital cameras race to outdo each other with ever-increasing numbers of megapixels. The world record for professional medium format digital cameras has now surpassed 60 megapixels per shot using a very large and expensive sensor with a resolution of about 9000 x 6700 pixels. Each time you capture such an image you get about 180 MB of uncompressed data and even more if you switch to 16-bit per colour for full dynamic range.
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Useful Links

Communities and Web Sources

www.researchgate.net/
Social network for scientists

www.ibiology.org/
Teaching tools, video lectures on biology and microscopy

bitesizebio.com
Online magazine and community for molecular and cell biology researchers

Search Engines and Data Bases

www.gopubmed.com
Search interface for pubmed

en.wikipedia.org/wiki/List_of_academic_databases_and_search_engines
List of academic databases and search engines

scholar.google.com
Beta of Google's search engine for scientific article abstracts

Journals

www.doaj.org/
Directory of open access journals

www.sciencemag.org/
Science

www.nature.com/
Nature

www.cell.com/
Biweekly publication of exceptional research articles

www.biotechniques.com/International Journal of Life Science Methods

www.opticsinfobase.org/
Collection of Journals and Proceedings in Optics and Photonics

spie.org/x576.xml
SPIE - peer-reviewed journals on applied research in optics and photonics

http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1864-0648
Journal of Biophotonics

www.plosone.org/home.action
International, peer-reviewed, open-access, online publication

www.microscopy-analysis.com/
International Journal for microscopists

Organizations / Institutes

www.microscopy.org/
Microscopy Society of America

www.eurmicsoc.org/
European Microscopy Society

www.rms.org.uk/
Royal Microscopical Society

www.ascb.org/
ASCB American Society of Cell Biology

www.biologists.com/cob_activities.html
the company of biologists

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