Learn & Share: Coherent Raman Scattering (CRS) Microscopy
Coherent Raman Scattering microscopy (CRS) describes a set of label-free methods that image structures by displaying the characteristic intrinsic vibrational contrast of their molecules. CRS comes in two popular variants, Coherent Anti-Stokes Raman Scattering (CARS) and Stimulated Raman Scattering (SRS). The crucial advantage of these methods is that they do not require labeling, and hence the sample remains almost unaffected. Instead, the image contrast arises from the spectroscopic properties of the various chemical species found in organic material.
Coherent Raman Scattering Microscopy Publication List
CRS (Coherent Raman Scattering) microscopy is an umbrella term for label-free methods that image biological structures by exploiting the characteristic, intrinsic vibrational contrast of their…Read article
Vibrational Spectroscopic Imaging in Cells, Tissues and Model Organisms
Here we present a webinar on the emerging applications of Stimulated Raman Scattering Microscopy, covering the following topics: Quantitative spectroscopic imaging of label-free or chemically tagged…Read article
How to prepare samples for Stimulated Raman Scattering
Stimulated Raman Scattering (SRS) is an emerging microscopy technique that images the vibrations of chemical bonds within a specimen. It allows researchers to probe the endogenous chemistry or…Read article
Step by Step Guide to the Molecular Basics of CARS Microscopy
CARS (Coherent Anti-Stokes Raman Scattering) microscopy is a dye-free method which images structures by displaying the characteristic intrinsic vibrational contrast of their molecules. The crucial…Read article
Stimulated Raman Scattering Microscopy Probes Neurodegenerative Disease
Despite decades of research, the molecular mechanisms underlying some of the most severe neurodegenerative diseases, such as Alzheimer’s or Parkinson’s, remain poorly understood. The progression of…Read article
Coherent Raman Scattering (CRS) Microscopy: A vast and growing range of applications revealed
Leica Microsystems‘ Workshop on Coherent Raman Scattering (CRS) Microscopy attracted 30 enthusiastic participants from all over the world to beautiful Konstanz, Germany! The group enjoyed a number of…Read article
Coupling of vinculin to F-actin requires Syndecan-4 proteoglycan
Heparan Sulfate Proteoglycans act as a cell surface co-receptors for many proteins in which a signal is sent downstream via its cytosolic domain. The role of syndecan-4 proteoglycans in FA assembly…Read article
Unmask the Hidden without Probes: CARS
Fluorescence microscopy assumed a pivotal role in cell biology once it was possible to stain cell components selectively by fluorescing dyes. One of the first explorers of targeted stainings, Paul…Read article
CARS Microscopy: Imaging Characteristic Vibrational Contrast of Molecules
Coherent anti-Stokes Raman scattering (CARS) microscopy is a technique that generates images based on the vibrational signatures of molecules. This imaging methods does not require labeling, yet…Read article
An Introduction to CARS Microscopy
CARS overcomes the drawbacks of conventional staining methods by the intrinsic characteristics of the method. CARS does not require labeling because it is highly specific to molecular compounds which…Read article
Good Vibrations
In recent years, new molecular imaging techniques, such as coherent anti-Stokes Raman scattering microscopy (CARS), have been developed for rapid vibrational imaging of living cells.Read article
CARS and Confocal
The most important drawback of single-photon and multiphoton confocal microscopy is the need to label the specimen. CARS (Coherent Anti-Stokes Raman Spectroscopy) addresses this issue because it is…Read article
Useful Links
Institutes
https://bernstein.harvard.edu/research/cars.htmlProf. Xiaoliang Sunney Xie, Havard Universitiy, Department of Chemistry and Chemical Biology
cms.uni-konstanz.de/zumbusch/Prof. Andreas Zumbusch, University of Konstanz, Department of Chemistry
chem.ps.uci.edu/~potma/webpage_test.htmAssistant Professor Eric Olaf Potma, Ph. D, University of California, Irvine (UCI)
Communities and Web Sources
www.researchgate.net/Social network for scientists
www.lsoft.com/scripts/wl.exe?SL1=CONFOCALMICROSCOPY&H=LISTS.UMN.EDUConfocal Microscopy Mailing List, University of Minnesota
bitesizebio.comOnline magazine and community for molecular and cell biology researchers
www.somersault1824.comResource for high-end scientific illustrations, images and animations
Search Engines and Data Bases
www.cellimagelibrary.orgPublic resource database of images, videos, and animations of cells
harvester.fzk.de/harvesterBioinformatic meta search engine for genes and proteins
www.gopubmed.comSearch interface for pubmed
en.wikipedia.org/wiki/List_of_academic_databases_and_search_enginesList of academic databases and search engines
scholar.google.comBeta of Google's search engine for scientific article abstracts
Journals
www.doaj.org/Directory of open access journals
www.lifescied.orgCBE-Life Sciences Education – an ASCB online journal
www.sciencemag.org/Science
www.nature.com/Nature
www.cell.com/Biweekly publication of exceptional research articles
jcs.biologists.org/Journal of Cell Science
dev.biologists.org/Development
jeb.biologists.org/The Journal of Experimental Biology
dmm.biologists.org/DMM Disease Models & Mechanisms
www.biotechniques.com/International Journal of Life Science Methods
www.opticsinfobase.org/Collection of Journals and Proceedings in Optics and Photonics
spie.org/x576.xmlSPIE - peer-reviewed journals on applied research in optics and photonics
http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1864-0648Journal of Biophotonics
www.plosone.org/home.actionInternational, peer-reviewed, open-access, online publication
rspb.royalsocietypublishing.org/Proceedings B - the Royal Society's biological research journal
www.microscopy-analysis.com/International Journal for microscopists
www.optoiq.com/index/biophotonics/biomedical-imaging/bow-microscopy.htmlNews and features on biomedical microscopy
Organizations
www.microscopy.org/Microscopy Society of America
www.eurmicsoc.org/European Microscopy Society
www.rms.org.uk/Royal Microscopical Society
www.ascb.org/ASCB American Society of Cell Biology
www.biologists.com/cob_activities.htmlthe company of biologists
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