Leica Science Lab - Topic : Coherent Raman Scattering (CRS) https://www.leica-microsystems.com//science-lab/topics/cars-microscopy/?tx_leicaacademy_pi2%5Baction%5D=listPagesForTopic&tx_leicaacademy_pi2%5Bcontroller%5D=Topic&tx_leicaacademy_pi2%5Btopic%5D=7&cHash=c915b27524e7733106995d3c2d392cb0 Article tagged with Coherent Raman Scattering (CRS) en-US https://www.leica-microsystems.com/28596 Coherent Raman Scattering (CRS) Vibrational Spectroscopic Imaging in Cells, Tissues and Model Organisms Here we present a webinar on the emerging applications of Stimulated Raman Scattering Microscopy, covering the following topics: Quantitative spectroscopic imaging of label-free or chemically tagged specimens: From cell and tissue biology to medical research, materials science, and microfabrication; Probing 3D cellular model systems (organoids) and tissues for preclinical & translational research in cancer, neurodegenerative diseases, immunology, and digestive disorders; Multi-modal confocal nonlinear optical microscopy, combining CARS, SRS, Second-Harmonic Generation, and 2-Photon Fluorescence for advanced tissue-based diagnostics; Monitoring tissue penetration of pharmacological and cosmetic compounds https://www.leica-microsystems.com/science-lab/vibrational-spectroscopic-imaging-in-cells-tissues-and-model-organisms/ Wed, 29 Apr 2020 08:46:00 +0000 Dr. Volker Schweikhard https://www.leica-microsystems.com/27674 Coherent Raman Scattering (CRS) Confocal Microscopy How to prepare samples for Stimulated Raman Scattering Stimulated Raman Scattering (SRS) is an emerging microscopy technique that images the vibrations of chemical bonds within a specimen. It allows researchers to probe the endogenous chemistry or biochemistry of their specimen without the need for exogenous labels. https://www.leica-microsystems.com/science-lab/how-to-prepare-samples-for-stimulated-raman-scattering/ Tue, 11 Feb 2020 08:59:00 +0000 Dr. Volker Schweikhard https://www.leica-microsystems.com/4592 Coherent Raman Scattering (CRS) Step by Step Guide to the Molecular Basics of CARS Microscopy CARS (Coherent Anti-Stokes Raman Scattering) microscopy is a dye-free method which images structures by displaying the characteristic intrinsic vibrational contrast of their molecules. The crucial advantage of this method is that the sample remains almost unaffected. This tutorial explains the molecular basics on Coherent Anti-Stokes Raman Scattering. https://www.leica-microsystems.com/science-lab/step-by-step-guide-to-the-molecular-basics-of-cars-microscopy/ Mon, 16 Dec 2019 11:38:00 +0000 Dr. Stefanie Degenhartt https://www.leica-microsystems.com/27272 Coherent Raman Scattering (CRS) Stimulated Raman Scattering Microscopy Probes Neurodegenerative Disease Despite decades of research, the molecular mechanisms underlying some of the most severe neurodegenerative diseases, such as Alzheimer’s or Parkinson’s, remain poorly understood. The progression of these diseases often correlates with the appearance of abnormal aggregations of proteins and lipids in affected brain tissues. There is hope that detailed investigations of these pathological structures might yield new insights into the disease mechanisms. Hence, there is a strong demand for advanced analytical techniques that can probe these structures in intact tissues. Here, we show that stimulated Raman scattering (SRS) microscopy can provide label-free, high-resolution, chemically specific characterization of tissue pathologies associated with neurodegenerative diseases. https://www.leica-microsystems.com/science-lab/stimulated-raman-scattering-microscopy-probes-neurodegenerative-disease/ Wed, 20 Nov 2019 11:32:00 +0000 Dr. Volker Schweikhard https://www.leica-microsystems.com/24931 Coherent Raman Scattering (CRS) Coherent Raman Scattering (CRS) Microscopy: A vast and growing range of applications revealed Leica Microsystems‘ Workshop on Coherent Raman Scattering (CRS) Microscopy attracted 30 enthusiastic participants from all over the world to beautiful Konstanz, Germany! The group enjoyed a number of great presentations, posters, and intense hands-on sessions on the latest Leica technology, including Coherent Anti-Stokes and Stimulated Raman Scattering microscopy (CARS/SRS), the FALCON lifetime imaging platform, and the THUNDER imagers. It was amazing to see a community of CARS/SRS users developing the technology in a physics lab and then using it for applications in biology, medicine, pharma, and much more. https://www.leica-microsystems.com/science-lab/summary-on-coherent-raman-scattering-crs-microscopy-workshop-in-konstanz-germany/ Sun, 28 Apr 2019 22:00:00 +0000 Dr. Volker Schweikhard https://www.leica-microsystems.com/19937 Coherent Raman Scattering (CRS) Coupling of vinculin to F-actin requires Syndecan-4 proteoglycan Heparan Sulfate Proteoglycans act as a cell surface co-receptors for many proteins in which a signal is sent downstream via its cytosolic domain. The role of syndecan-4 proteoglycans in FA assembly was studied in endothelial cells, and single molecule localization super-resolution microscopy showed that Syn4 loss altered the cytoskeleton protein network assembly. Using molecular and cell biology approaches we were able to demonstrate that syndecan-4 plays a pivotal role in endothelial cell behavior. Super-resolution microscopy revealed that syndecan-4 knockdown alters the level and arrangement of essential proteins for focal adhesion, including actin network and a selective decoupling of vinculin from actin, leading to lamellipodia assembly and filopodia protrusions (Figure 1). Furthermore, using Coherent Anti-stokes Raman Scattering (CARS) microscopy, the structure of the tumors formed by such cells in mice was revealed showing its network of vascularization (Figure 2). We propose a model in which syndecan-4 acts as a central mediator: serving as a cell-signaling center in which ECM (fibronectin), cell surface (integrins) and intracellular components (actin and vinculin) come together. https://www.leica-microsystems.com/science-lab/coupling-of-vinculin-to-f-actin-requires-syndecan-4-proteoglycan/ Wed, 07 Feb 2018 23:00:00 +0000 https://www.leica-microsystems.com/9374 Coherent Raman Scattering (CRS) Unmask the Hidden without Probes: CARS Fluorescence microscopy assumed a pivotal role in cell biology once it was possible to stain cell components selectively by fluorescing dyes. One of the first explorers of targeted stainings, Paul Ehrlich, had the idea that something that stains specifically should also kill specifically – which was associated with the term “magic bullet”, the essential idea of chemotherapy. His group discovered Salvarsan, a tailored drug against syphilis – though not specific enough not to cause substantial side effects. Screening many fluorescent dyes led to a long list of stainings which are used in histology, including dyes like DAPI or hematoxylin and eosin. https://www.leica-microsystems.com/science-lab/unmask-the-hidden-without-probes-cars/ Thu, 21 Mar 2013 23:00:00 +0000 Dr. Rolf T. Borlinghaus, Dr. Stefanie Degenhartt https://www.leica-microsystems.com/5901 Coherent Raman Scattering (CRS) CARS Microscopy: Imaging Characteristic Vibrational Contrast of Molecules Coherent anti-Stokes Raman scattering (CARS) microscopy is a technique that generates images based on the vibrational signatures of molecules. This imaging methods does not require labeling, yet molecular specific information can be obtained from a range of important bio-molecular compounds. https://www.leica-microsystems.com/science-lab/cars-microscopy-imaging-characteristic-vibrational-contrast-of-molecules/ Mon, 16 Jul 2012 22:00:00 +0000 Ph.D. Eric Olaf Potma