Leica Science Lab - Topic : CLEM https://www.leica-microsystems.com//science-lab/topics/clem/?tx_leicaacademy_pi2%5Baction%5D=listPagesForTopic&tx_leicaacademy_pi2%5Bcontroller%5D=Topic&tx_leicaacademy_pi2%5Btopic%5D=143&cHash=8ceb529bba5131f23e175301eb709c24 Article tagged with CLEM en-US https://www.leica-microsystems.com/45807 CLEM EM Sample Preparation Advancing Cell Biology with Cryo-Correlative Microscopy Correlative light and electron microscopy (CLEM) advances biological discoveries by merging different microscopes and imaging modalities to study systems in 4D. Combining fluorescence microscopy with cryo-electron tomography allows investigators to bridge knowledge gaps between cell and structural biology. CLEM technologies are rapidly advancing in order to improve biological understanding, experimental reproducibility, and automation. https://www.leica-microsystems.com/advancing-cell-biology-with-cryo-correlative-microscopy/ Thu, 28 Jan 2021 08:54:00 +0000 Corporate Communications https://www.leica-microsystems.com/30159 CLEM EM Sample Preparation Widefield Microscopy Crystal clear cryo light-microscopy images This article describes how computational clearing of cryo light microscopy images improves the identification of cellular targets for cryo electron-microscopy. https://www.leica-microsystems.com/science-lab/crystal-clear-cryo-light-microscopy-images/ Mon, 20 Jul 2020 13:21:00 +0000 Dr. Jan De Bock, Dr. Jan Schumacher, Louise Bertrand https://www.leica-microsystems.com/30136 Confocal Microscopy Fluorescence Microscopy Super-Resolution CLEM TIRF Microscopy Widefield Microscopy Live-Cell Imaging Laser Microdissection Microscopy in Virology The coronavirus SARS-CoV-2, causing the Covid-19 disease effects our world in all aspects. Research to find immunization and treatment methods, in other words to fight this virus, gained highest priority in 2020. Microscopes play a significant role in this kind of research. To understand the underlaying principles of receptor binding, genome release, replication, assembly, and virus budding, as well as the response of our immune system, different methodologies and microscopes can be used. This article summarizes why microscopy is an important tool in virology and infection biology and gives examples for different microscopy technologies and their applications in these research fields. https://www.leica-microsystems.com/science-lab/microscopy-in-virology/ Mon, 13 Jul 2020 12:13:00 +0000 Dr. Christoph Greb https://www.leica-microsystems.com/29634 EM Sample Preparation CLEM Introduction to Ion Beam Etching with the EM TIC 3X In this article you can learn how to optimize the preparation quality of your samples by using the ion beam etching method with the EM TIC 3X ion beam milling machine. A short introduction of the instrument explains how the flexible setup of the EM TIC 3X gives you the opportunity to prepare samples for various investigation areas. https://www.leica-microsystems.com/science-lab/introduction-to-ion-beam-etching-with-the-em-tic-3x/ Mon, 11 May 2020 13:15:00 +0000 https://www.leica-microsystems.com/27911 CLEM Improve Cryo Electron Tomography Workflow Leica Microsystems and Thermo Fisher Scientific have collaborated to create a fully integrated cryo-tomography workflow that responds to these research needs: Reveal cellular mechanisms at subnanometer resolution in 3D applying the fully integrated cryo electron tomography workflow https://www.leica-microsystems.com/science-lab/improve-cryo-electron-tomography-workflow/ Tue, 03 Sep 2019 09:50:00 +0000 https://www.leica-microsystems.com/18008 CLEM Fluorescence Microscopy EM Sample Preparation Correlative Cryo-Fluorescence and Cryo-Scanning Electron Microscopy as a Straightforward Tool to Study Host-Pathogen Interactions Correlative light and electron microscopy is an imaging technique that enables identification and targeting of fluorescently tagged structures with subsequent imaging at near-to-nanometer resolution. We established a novel correlative cryo-fluorescence microscopy and cryo-scanning electron microscopy workflow, which enables imaging of the studied object of interest very close to its natural state, devoid of artifacts caused for instance by slow chemical fixation. This system was tested by investigating the interaction of the zoonotic bacterium Borrelia burgdorferi with two mammalian cell lines of neural origin in order to broaden our knowledge about the cell-association mechanisms that precedes the entry of the bacteria into the cell. https://www.leica-microsystems.com/science-lab/correlative-cryo-fluorescence-and-cryo-scanning-electron-microscopy-as-a-straightforward-tool-to-study-host-pathogen-interactions/ Tue, 13 Dec 2016 07:58:00 +0000 Martin Strnad https://www.leica-microsystems.com/17382 CLEM Imaging of Host Cell-bacteria Interactions using Correlative Microscopy under Cryo-conditions Pathogenic bacteria have developed intriguing strategies to establish and promote infections in their respective hosts. Most bacterial pathogens initiate infectious diseases by adhering to host cells surface. Knowledge of the interplay between the pathogenic organism and the host cells can provide fundamental new insight into the underlying mechanisms of the infectious process and therefore of the disease. Various microscopy techniques have proven to be critical tools to study these events. https://www.leica-microsystems.com/science-lab/imaging-of-host-cell-bacteria-interactions-using-correlative-microscopy-under-cryo-conditions/ Tue, 01 Mar 2016 11:34:00 +0000 Martin Strnad https://www.leica-microsystems.com/15509 CLEM Correlative In-Resin Super-Resolution and Electron Microscopy Using Standard Fluorescent Proteins We introduce a method for correlative in-resin super-resolution fluorescence and electron microscopy (EM) of biological structures in mammalian culture cells. Cryo-fixed resin embedded samples offer superior structural preservation, performing in-resin super-resolution, however, remains a challenge. https://www.leica-microsystems.com/science-lab/correlative-in-resin-super-resolution-and-electron-microscopy-using-standard-fluorescent-proteins/ Thu, 04 Jun 2015 10:28:00 +0000 https://www.leica-microsystems.com/13642 CLEM EM Sample Preparation Correlative Light and Electron Microscopy – Get Your Free CLEM e-Book for Download The urge to go deeper into the microscopic world has led researchers to combine the versatility of the light microscopy (LM) with the resolution power of the electron microscope (EM) to produce Correlative Light and Electron Microscopy (CLEM). CLEM’s most significant and powerful characteristic for cell biology research is an ability to study the same cell using two different microscopy platforms. The Essential Knowledge Briefing describes the basics of CLEM and also reviews potential pitfalls and problems as well as tricks for solving them. https://www.leica-microsystems.com/science-lab/correlative-light-and-electron-microscopy-get-your-free-clem-e-book-for-download/ Thu, 28 May 2015 16:34:00 +0000 Professor Paul Verkade https://www.leica-microsystems.com/14920 Multiphoton Microscopy CLEM Live-Cell Imaging Correlating Intravital Multi-Photon Microscopy to 3D Electron Microscopy of Invading Tumor Cells Using Anatomical Reference Points Cancer research unsing multiphoton microscopy and 3D electron microscopy. Correlative microscopy combines the advantages of both light and electron microscopy to enable imaging of rare and transient events at high resolution. Performing correlative microscopy in complex and bulky samples such as an entire living organism is a time-consuming and error-prone task. https://www.leica-microsystems.com/science-lab/correlating-intravital-multi-photon-microscopy-to-3d-electron-microscopy-of-invading-tumor-cells-using-anatomical-reference-points/ Fri, 24 Apr 2015 13:06:00 +0000