Learn & Share: Correlative Light Electron Microscopy (CLEM)
CLEM (Correlative Light Electron Microscopy) combines the capabilities of two typically separate microscopy platforms: light (or fluorescent) microscopy (LM) and electron microscopy (EM). The advantage of LM is that it can provide wide field images of whole, often living, cells, but its resolution is limited. The advantage of EM is that it can provide much higher resolution images, up to molecular dimensions, but only over specific regions of a cell at a time and not in living cells. CLEM combines the advantages of both techniques, allowing scientists to spot cellular structures and processes of interest in whole cell images with LM and then zoom in for a closer look with EM. This dual examination provides valuable complementary and often unique information.
Crystal clear cryo light-microscopy images
This article describes how computational clearing of cryo light microscopy images improves the identification of cellular targets for cryo electron-microscopy.Read article
Microscopy in Virology
The coronavirus SARS-CoV-2, causing the Covid-19 disease effects our world in all aspects. Research to find immunization and treatment methods, in other words to fight this virus, gained highest…Read article
Improve Cryo Electron Tomography Workflow
Leica Microsystems and Thermo Fisher Scientific have collaborated to create a fully integrated cryo-tomography workflow that responds to these research needs: Reveal cellular mechanisms at…Read article
Imaging of Host Cell-bacteria Interactions using Correlative Microscopy under Cryo-conditions
Pathogenic bacteria have developed intriguing strategies to establish and promote infections in their respective hosts. Most bacterial pathogens initiate infectious diseases by adhering to host cells…Read article
New Insights into Cilia and Flagella by Cryo-EM
Cilia and flagella were the first organelles to be discovered and have been studied for centuries. However, their essential role in humans and how ciliary defects cause diseases are still not well…Read article
CLEM: Combining the Strengths of Light and Electron Microscopy
In recent years light microscopy studies have been dominated by live cell imaging while electron microscopy has been used for high-resolution studies. Latterly, there has been increasing interest in…Read article
Useful Links
Communities
www.researchgate.net/Social network for scientists
bitesizebio.comOnline magazine and community for molecular and cell biology researchers
Search Engines and Data Bases
www.cellimagelibrary.orgPublic resource database of images, videos, and animations of cells
harvester.fzk.de/harvesterBioinformatic meta search engine for genes and proteins
www.gopubmed.comSearch interface for pubmed
en.wikipedia.org/wiki/List_of_academic_databases_and_search_enginesList of academic databases and search engines
scholar.google.comBeta of Google's search engine for scientific article abstracts
www.somersault1824.comResource for high-end scientific illustrations, images and animations
Web Sources
https://www.elsevier.com/books/correlative-light-and-electron-microscopy/unknown/978-0-12-416026-2Methods in Cell Biology – Vol. 111: Correlative Light and Electron Microscopy (Mueller-Reichert T, Verkade P)
http://my.safaribooksonline.com/book/-/9781118271926/chapter-6-toward-a-3d-view-of-cellular-architecture-correlative-light-microscopy/c06_level1_5_xhtml Biomedical Imaging: Principles and Applications (Salzer R), Chapter 6.5: CLEM Applications
https://openaccess.leidenuniv.nl/handle/1887/17922 Leiden University: Correlative Light and Electron Microscopy: Strategies and Applications
http://teemcof.ibp.cnr.it/services/correlative-light-electron-microscopyThe TeleThon Electron Microscopy Core Facility (TeEMCoF): Correlative Light-Electron Microscopy
www.embl.de/research/units/cbb/schwab/Volume Correlative Light and Electron Microscopy
www.emez.ethz.ch/education/pastevents/pastev/Correl_LMEM_rw.pdf Correlative Microscopy in Focus: Zoom-in Beyond LM …
http://jcb.rupress.org/content/192/1/111.full Correlated Fluorescence and 3D Electron Microscopy with High Sensitivity and Spatial Precision
www.cellbiology-utrecht.nl/second-menu-cmc/techniques.html Cell Microscopy Center: Techniques
www.biocenter.helsinki.fi/bi/em/emu_methods_clem.html Methods: Correlative Light Electron Microscopy
www.i-bioimaging.it/images/Alberto%20Luini.pdf Correlative Microscopy
http://jcs.biologists.org/content/early/2012/06/05/jcs.090027.full.pdf Advances in High-resolution Imaging – Techniques for Three-dimensional Imaging of Cellular Structures
www.sartre.ac.uk/sites/default/files/Verkade.pdf A Single Probe to Combine 2 Imaging Modalities
www.imaging-git.com/science/light-microscopy/clem-ultrathin-sections CLEM on Ultrathin Sections – From Micro- to Nanoscale
www.formatex.info/microscopy4/277-285.pdf Correlative Light and Electron Microscopic Exploration of Endocytic Organelles
www.emc2012.org.uk//documents/Abstracts/Abstracts/EMC2012_1163.pdf In and Out of Mitosis – Correlative Light and Electron Microscopy for Structural Cell Biology
http://users.unimi.it/~cima/bibliocorrelative/jmicroscopy2007.pdf Cryo-fluorescence Microscopy Facilitates Correlations between Light and Cryo-electron Microscopy and Reduces the Rate of Photobleaching
www.cgl.ucsf.edu/Research/auer/ Correlative Light and Electron Microscopy: Visualization of Molecular Machines in Their Native Cell and Tissue Context
www.labmate-online.com/articles/microscopy-and-microtechniques/4/paul_verkade/the_use_of_high-pressure_freezing_in_correlative_light_electron_microscopy/71/ The Use of High-pressure Freezing in Correlative Light Electron Microscopy
http://de.scribd.com/doc/156152708/Photooxidation-Technology-for-Correlative-Light-and-Electron-Microscopy Photooxidation Technology for Correlative Light and Electron Microscopy
http://walsworth.physics.harvard.edu/publications/2012_Glenn_ArXiv.pdf Multi-color Correlative Light and Electron Microscopy Using Nanoparticle Cathodoluminescence
Organizations
www.microscopy.org/Microscopy Society of America
www.eurmicsoc.org/European Microscopy Society
www.rms.org.uk/Royal Microscopical Society
www.ascb.org/ASCB American Society of Cell Biology
www.biologists.com/cob_activities.htmlthe company of biologists
Your bookmarks are missing?
Please send us your useful links!