Multi-photon Excitation Microscopy
Advanced microscopical techique for life science: multiphoton microscopy. Multi-photon excitation (MPE) microscopy plays a growing role among microscopical techniques utilized for studying biological…Read article
FRET Sensitized Emission Wizard Confocal
Förster Resonance Energy Transfer (FRET) is a technique, which allows insight into the interactions between proteins or molecules in proximities beyond light microscopic resolution.Read article
4D Photoactivation of pa-GFP in Living Cells Using Two-Photon Excitation Laser Scanning Microscopy
We report about two-photon activation of a photoactivatable derivative of the Aequorea Victoria green fluorescent protein (pa-GFP). This special form of the molecule increases its fluorescence…Read article
Characterization of Photosynthetic Biofilms from Roman Catacombs via 3D Imaging and Subcellular Identification of Pigments
Artificial illumination can harm hypogean monuments by inducing the uncontrolled growth of photosynthetic biofilms (green sickness or maladie verte). With the aim of preventing biodeterioration or…Read article
In the Footsteps of Linnaeus – Microscopic Imaging in Marine Biodiversity Research
For the study of minute marine organisms such as free swimming larvae or bottom dwelling invertebrates it is a great advantage to have modern microscopic equipment in close proximity to the natural…Read article
Obese and Slim Yeast Cells
Lipids are on everyone’s lips nowadays, whether ω-3/6 fatty acids, good and bad cholesterol or just plain fat that has the annoying habit of accumulating on our hips. Serious diseases such as obesity,…Read article
From Molecules to Tissues
Cancer research using confocal and multiphoton microscopy. Sequencing of the human genome stimulated a radical change in the approach to biomedical research. The comprehension of the mechanisms…Read article
The First Supercontinuum Confocal that Adapts to the Sample
Until now, biological and medical research fluorescence imaging in multi-user facilities or institutes has been limited by the type or number of dyes that could be excited. The Leica TCS SP5 X…Read article
Apical Cargo Traverses Endosomal Compartments on the Passage to the Cell Surface
Epithelial polarity is based on intracellular sorting machinery that maintains the asymmetric distribution of lipids and proteins to the cell surface. Dependent on their lipid raft affinity, newly…Read article
Stem Cell Biology in Cancer Research
The comprehension of stem cell biology and its molecular basis is now acquiring paramount importance in cancer research. The need to look at a single, possibly living, cell makes fluorescence…Read article
CARS and Confocal
The most important drawback of single-photon and multiphoton confocal microscopy is the need to label the specimen. CARS (Coherent Anti-Stokes Raman Spectroscopy) addresses this issue because it is…Read article
Deep Tissue Imaging
Developmental biology using Multiphoton microscopy with OPO. To gain new insight into the fundamental control of cell response to physical changes and to study the dynamics and roles of biological…Read article
Exploring the Concert of Neuronal Activities
Brain research using Confocal and Multiphoton Microscopy. Using imaging techniques such as confocal and two-photon microscopy, neuronal dendritic arborization of neurons and their synaptic…Read article
Choose Your Excitation Wavelength
Although time correlated single photon counting (TCSPC) is the method of choice for fluorescence lifetime quantification, it requires dedicated instrumentation including a pulsed laser source, a…Read article
Confocal Microscopy - Optical Path
“Confocal Microscopy” refers to a particular optical microscope that allows recording optical sections. Optical sectioning is achieved in a confocal system by illuminating and observing a single…Read article
Organotypic Cerebellar Cultures: Apoptotic Challenges and Detection
Organotypic cultures of neuronal tissue were first introduced by Hogue in 1947 and have constituted a major breakthrough in the field of neuroscience. Since then, the technique was developed further…Read article
Confocal Optical Section Thickness
Confocal microscopes are employed to optically slice comparably thick samples.Read article
Pinhole Controls Optical Slicing
True confocal scanning microscopes (TCS) use a variable detection pinhole. Good optical sectioning tries to use just the inner core of the PSF.Read article
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