A Quality Metric for the Systematic Evaluation of Clearing Protocols
3D multicellular spheroids are of interest for studying tumor behavior and evaluating the response of pharmacologically active agents, because they mimic the in vivo tumor environment better than…Read article
How FLIM Microscopy helps to detect microplastic pollution
The use of autofluorescence in biological samples is a widely used method to gain detailed knowledge about systems or organisms. This property is not only found in biological systems, but also…Read article
A New World of Confocal Applications with the Next Generation White Light Lasers
As biological questions get more complex, there is an increasing need to study multiple events simultaneously in the same specimen. When preparing the specimen for imaging experiments, this need is…Read article
Advanced Quantitative Fluorescence Microscopy to Probe the Molecular Dynamics of Viral Entry
Viral entry into the host cell requires the coordination of many cellular and viral proteins in a precise order. Modern microscopy techniques are now allowing researchers to investigate these…Read article
How to Quantify Label-free Blood Flow
The application note “Label-free Blood Flow Quantification” is a guide for data acquisition and analysis of blood flow using label-free third harmonic generation (THG) imaging.Read article
How Can Immunofluorescence Aid Virology Research?
Modern virology research has become as crucial now as ever before due to the global COVID-19 pandemic. There are many powerful technologies and assays that virologists can apply to their research into…Read article
Obtain maximum information from your specimen with LIGHTNING
LIGHTNING is an adaptive process for extraction of information that reveals fine structures and details, otherwise simply not visible, fully automatically. Unlike traditional technologies, that use a…Read article
Infection of the novel coronavirus SARS-CoV-2 can be blocked by a human monoclonal antibody
Vaccines and targeted therapeutics for treatment of the respiratory disease COVID-19, caused by the coronavirus SARS-CoV-2, are currently lacking. Based on the results of this study, it was reported…Read article
Expanding the frontiers of confocal live cell imaging
To address the specific challenges of live cell confocal imaging and help researchers get closer to the truth, Leica Microsystems has re-imagined confocal microscopy, designing its entirely new…Read article
Microscopy in Virology
The coronavirus SARS-CoV-2, causing the Covid-19 disease effects our world in all aspects. Research to find immunization and treatment methods, in other words to fight this virus, gained highest…Read article
Nanoscopy meets Lifetime: Introducing τ-STED
τ-STED combines the optical signals from STED and the photophysical information from the fluorescence lifetime at unprecedented speeds. This approach to STED uses phasor analysis in a novel way.…Read article
Explore Innovative Techniques to Separate Fluorophores with Overlapping Spectra
In this article we explore several strategies you can take to improve the separation of fluorophores and increase the number of fluorescent probes you can distinguish in your sample.Read article
Add an Extra Dimension to Confocal Results with TauSense
Leica Microsystems’ TauSense technology is a set of imaging modes based on fluorescence lifetime. Found at the core of the STELLARIS confocal platform, it will revolutionize your imaging experiments.…Read article
How to Uncover Hidden Dimensions in Research with Lifetime Imaging
Learn how fluorescence lifetime imaging adds information depth to your confocal experiments and reveals novel insights that are difficult or impossible to discover using conventional intensity-based…Read article
Learn how to Remove Autofluorescence from your Confocal Images
Autofluorescence can significantly reduce what you can see in a confocal experiment. This article explores causes of autofluorescence as well as different ways to remove it, from simple media fixes to…Read article
Multiphoton Microscopy Publication List
Multiphoton Microscopy is an advanced technique for imaging thick samples. Applications range from the visualization of the complex architecture of the whole brain to the study of tumor development…Read article
How to prepare samples for Stimulated Raman Scattering
Stimulated Raman Scattering (SRS) is an emerging microscopy technique that images the vibrations of chemical bonds within a specimen. It allows researchers to probe the endogenous chemistry or…Read article
Pore Scale Visualization of Drainage in 3D Porous Media by Confocal Microscopy
This work reports new interesting findings concerning porous media infiltration which have direct implications for the fields of energy (oil recovery) and environment (CO2 sequestration and aquifer…Read article
Multiphoton imaging bolsters findings on in vitro differentiation of pluripotent stem cell-derived podocyte
The renal corpuscle of the kidney comprises a glomerular vasculature embraced by podocytes and supported by mesangial myofibroblasts. Their combined actions are essential for the formation of an…Read article
Cellular Motility: Microtubules, Motor Proteins and Tau-Proteins
Cellular motility is based on motor-proteins that can bind to filamentous scaffold proteins and – under consumption of ATP – can “crawl” on these filaments. This note is about proteins connected to…Read article
Visualize developmental patterns in Drosophila
The marvelous complexity of life is perhaps the greatest source of fascination within the biological sciences. Among the most complex subjects within biology is the development of multicellular life.…Read article
Visualize mechanical interactions of Cancer Cells
Cells interact with their environment. Not only on the base of chemical signals, but also by sensing and modifying mechanical properties of the extracellular matrix. The research goal of Dr. Bo Sun’s…Read article
Zebrafish Brain - Whole Organ Imaging at High Resolution
Structural information is key when one seeks to understand complex biological systems, and one of the most complex biological structures is the vertebrate central nervous system. To image a complete…Read article
What is a Resonant Scanner?
A resonant scanner is a type of galvanometric mirror scanner that allows fast image acquisition with single-point scanning microscopes (true confocal and multiphoton laser scanning). High acquisition…Read article
What is a Spectral Detector (SP Detector)?
The SP detector from Leica Microsystems denotes a compound detection unit for point scanning microscopes, in particular confocal microscopes. The SP detector splits light into up to 5 spectral bands.…Read article
Improve 3D Cell Biology Workflow with Digital Light Sheet Microscopy
Understanding the sub-cellular mechanisms in carcinogenesis is of crucial importance for cancer treatment. Popular cellular models comprise cancer cells grown as monolayers. But this approach…Read article
Improve Workflow in Kidney Research with 3D STED Deep Nanoscopy
Visualize the ultrastructure of the glomerular filtration barrier. The combination of optical clearing and nanoscopy allows to study the glomerular filtration barrier at the nanoscale. The Leica TCS…Read article
Resolved Field Number (RFN)
The field number (FN) for optical microscopes indicates the field of view (FOV). It corresponds to the area in the intermediate image that is observable through the eyepieces. Although, we cannot…Read article
What is a Hybrid Detector (HyD)?
A HyD is a sensor for detecting light (photons). It is a compound (hybrid) of two technologies: vacuum tubes, like those used in a photomultiplier tube (PMT), and semiconductor microelectronics, like…Read article
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