What is CRISPR-Cas9?
Working with DNA is a common base for almost all life science branches. The manipulation of genes is often the method of choice to get insight into cellular processes. Thus DNA-editing proteins which are able to bind and open DNA strands site-specifically are of great importance. There are several to choose from, and all have their advantages and disadvantages. The Cas9 endonuclease of the bacterial derived CRISPR-Cas9 immune system is one of them. In contrast to its alternatives – meganucleases, ZFNs, and TALENs – the Cas9 DNA-binding determinant is an RNA molecule, which is easy to design. Thus Cas9 is very flexible concerning target sequence choice. Therefore it is frequently used to mutate DNA, and to produce knock-out and knock-in mutants.
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Role of circadian gene Clock during differentiation of mouse pluripotent stem cells
Biological rhythms controlled by the circadian clock are absent in embryonic stem cells (ESCs). However, they start to develop during the differentiation of pluripotent ESCs to downstream cells.…Read article -
Video: Genome Engineering with CRISPR-Cas9
Jennifer Doudna tells the story of how studying the way bacteria fight viral infection turned into a genomic engineering technology that has transformed molecular biology research. In 2013, Doudna and…Read article -
Video: Genome Editing with CRISPR-Cas9
Jennifer Doudna tells the story of how studying the way bacteria fight viral infection turned into a genomic engineering technology that has transformed molecular biology research. In 2013, Doudna and…Read article -
Five Big Mysteries about CRISPR’s Origins
Francisco Mojica was not the first to see CRISPR, but he was probably the first to be smitten by it. He remembers the day in 1992 when he got his first glimpse of the microbial immune system that…Read article -
Gene Editing with CRISPR/Cas9 - Breakthrough in Genome Engineering
The CRISPR/Cas9 system is one of several different bacterial systems for defense against viral attacks. It consists of two main components. One is a small piece of RNA which binds to the viral target…Read article -
Video Talk: CRISPR-Cas – From a Bacterial Adaptive Immune System to a Genome Engineering Tool
The CRISPR-Cas system was originally discovered as an adaptive immune system of bacteria and archaea to protect against viral attack. During this talk, given at Leica Microsystems in Wetzlar, Dr.…Read article -
CRISPR/Cas9-mediated Endogenous Protein Tagging for RESOLFT Super-Resolution Microscopy of Living Human Cells
Overexpression is a notorious concern in conventional and especially in super-resolution fluorescence light microscopy studies because it may cause numerous artifacts including ectopic sub-cellular…Read article