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Plant Cell Development and Morphogenesis

Authors: <a rel="author" href="/science-lab/authors/detail/?tx_leicaacademy_pi3%5Baction%5D=show&amp;tx_leicaacademy_pi3%5Bauthor%5D=2922&amp;tx_leicaacademy_pi3%5Bcontroller%5D=author&amp;cHash=a6b664a12740a4cc69460aa0f5746849"> Olga Davydenko , PhD </a> , <a rel="author" href="/science-lab/authors/detail/?tx_leicaacademy_pi3%5Baction%5D=show&amp;tx_leicaacademy_pi3%5Bauthor%5D=1779&amp;tx_leicaacademy_pi3%5Bcontroller%5D=author&amp;cHash=8aeec44ebbbb442b1f1efd9fe4c0eb5f"> James DeRose , PhD </a>
Jan 21, 2021
The article discusses how tubulin molecules, which make up microtubules, in plant cells can be studied with total internal reflection fluorescence (TIRF) microscopy to better understand tubulin dynamics and cell development and morphogenesis. To visualize tubulin molecules in microtubules, an imaging solution is needed that allows them to be easily resolved near the plant cell surface. Conventional widefield microscopy detects too much out-of-focus fluorescence signal leading to reduced resolution. TIRF microscopy minimizes out-of-focus fluorescence and enables the tubulin to be clearly resolved, making TIRF a useful tool for studying molecular dynamics. Read article