Workflows & Protocols: How to Use a Leica Laser Microdissection System and Qiagen Kits for Successful RNA Analysis
Laser Microdissection (LMD) allows isolating individual cells or chromosomes and is a well established technique for sample preparation prior downstream analysis of the nucleic acid content via PCR or…Read article
Consumables for Laser Microdissection
There are many different types of consumables for laser microdissection (LMD) systems. They cover a wide range of applications from basic to highly specialized, enabling scientists to choose their own…Read article
Total RNA Isolation after Laser-capture Microdissection of Human Cervical Squamous Epithelial Cells from Fresh Frozen Tissue
As most tissue specimens contain a mixture of different cell types including epithelial cells, stromal cells and immune cells, selection of the cells of interest is of utmost importance for the…Read article
Customized Patterned Substrates for Highly Versatile Correlative Light-Scanning Electron Microscopy
Correlative light electron microscopy (CLEM) combines the advantages of light and electron microscopy, thus making it possible to follow dynamic events in living cells at nanometre resolution. Various…Read article
Identification of Molecular Pathways Facilitating Glioma Cell Invasion In Situ
Gliomas are mostly incurable secondary to their diffuse infiltrative nature. Thus, specific therapeutic targeting of invasive glioma cells is an attractive concept. As cells exit the tumor mass and…Read article
Glucose-Regulated Protein 78 (Grp78) Confers Chemoresistance to Tumor Endothelial Cells under Acidic Stress
This study was designed to investigate the activation of the unfolded protein response (UPR) in tumor associated endothelial cells (TECs) and its association with chemoresistance during acidic pH…Read article
Preparation of Laser Microdissected Plant Cell and Tissue Material for Microchemical Analysis
Secondary plant metabolites are involved in various plant physiological and biochemical processes, allowing plants to interact successfully with their biotic and abiotic environment. Distribution…Read article
The Morbus Parkinson Puzzle
A characteristic sign of M. Parkinson is the deterioration of dopaminergic neurons in the mid-brain, specifically in the substantia nigra (SN, black substance). Different causes and forms of this…Read article
Production of RNA for Transcriptomic Analysis from Mouse Spinal Cord Motor Neuron Cell Bodies by Laser Capture Microdissection
High-quality total RNA has been prepared from cell bodies of mouse spinal cord motor neurons by laser capture microdissection after staining spinal cord sections with Azure B in 70% ethanol.…Read article
Laser Microdissection (LMD) and Fancy Applications
New and far-reaching applications have recently been opened up in the field of laser microdissection. In addition to conventional dissection, the laser microdissection system (LMD) of Leica…Read article
Video: How to Achieve Fast Precise Cutting Lines in Laser Microdissection
This video explains the laser beam deflection principle of Leica Microsystems‘ laser microdissection systems (Leica LMD). The laser, the beam expander, motorized attenuator, motorized iris diaphragm…Read article
From Dynamic Live Cell Imaging to 3D Ultrastructure
To correlate dynamic events in adherent cells with both ultrastructural and 3D information, we developed a method for cultured cells that combines confocal time-lapse images of GFP-tagged proteins…Read article
Quantitative One-Step Protocol to Detect Transcripts in Laser Microdissected Samples
Arbuscular mycorrhizal (AM) fungi are an essential feature of the biology and ecology of most terrestrial plants and, as biofertilizers, AM fungi are an emerging issue in many projects focused on…Read article
Stem Cells in the Aging Process – the Signal Protein p53 as "Guardian of the Genome"
The physiological process of aging is an elementary part of life. The processes responsible for the aging process and the effect they have is one of the key focuses of biomedical research. Dr. Yvonne…Read article
K-ATP Channels in Dopamine Substantia Nigra Neurons Control Bursting and Novelty-induced Exploration
Phasic activation of the dopamine (DA) midbrain system in response to unexpected reward or novelty is critical for adaptive behavioral strategies. This activation of DA midbrain neurons occurs via a…Read article
Comparison of the RNA Quality of Native Tissue Samples with Tissue Samples after UV Laser Microdissection
Due to its instability, RNA is generally more difficult to work with than DNA. RNA does not have the stability of the DNA double helix. To obtain the best results when dealing with RNA it is essential…Read article
Region-Specific Gene Expression in Adult Mouse CNS Tissues
Different areas of the Central Nervous System (CNS) display a specific and selective gene expression profile. Here, we used the Laser Microdissection system Leica LMD6500 to study region-specific mRNA…Read article
TED Talk of Allan Jones: Mapping the Human Brain
How can we begin to understand the complexity of the human brain and the way it works? The same way we begin to understand a city: by making a map. In this talk, Allan Jones shows how his team is…Read article
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