Gaining Insights into Colorectal Cancer Metastasis
To follow cancer metastasis at the point of occurrence intravital multiphoton microscopy is the tool of choice. Intravital microscopy allows researchers to obtain image data from a living animal, even…Read article
Accessing the Metabolic Information of Stem Cells
Cancer often comes with changes in the metabolism of cells. Monitoring the metabolic state using fluorescence lifetime imaging microscopy (FLIM) is a good way to assess these changes. In some cases,…Read article
How to Quantify Label-free Blood Flow
The application note “Label-free Blood Flow Quantification” is a guide for data acquisition and analysis of blood flow using label-free third harmonic generation (THG) imaging.Read article
Multiphoton Microscopy Publication List
Multiphoton Microscopy is an advanced technique for imaging thick samples. Applications range from the visualization of the complex architecture of the whole brain to the study of tumor development…Read article
Principles of Multiphoton Microscopy for Deep Tissue Imaging
Basics of multiphoton microscopy. This tutorial explains the principles of multiphoton microscopy for deep tissue imaging. Multiphoton microscopy uses excitation wavelengths in the infrared taking…Read article
Chat with Dr. Thomas Mathivet about In Vivo Cardiovascular Imaging of the Brain
Dr. Thomas Mathivet, working at the Cardiovascular Institute in Paris, France, was interviewed by Dr. Luis Alvarez, Product Application Manager, during Leica Microsystems Meets Science 2019 in…Read article
Multiphoton imaging bolsters findings on in vitro differentiation of pluripotent stem cell-derived podocyte
The renal corpuscle of the kidney comprises a glomerular vasculature embraced by podocytes and supported by mesangial myofibroblasts. Their combined actions are essential for the formation of an…Read article
SP8 DIVE Multiphoton Microscope Image Gallery
Today’s life science research focusses on complex biological processes, such as the causes of cancer and other human diseases. A deep look into tissues and living specimens is vital to understanding…Read article
High-Resolution 3D Imaging of Whole Organ after Clearing
Zebrafish testis has become a powerful model for reproductive biology of teleostean fishes and other vertebrates and encompasses multiple applications in applied and basic research. Many studies have…Read article
Multiphoton Microscopy – a Satisfied Wish List
The colorful picture shows colon tumor cells, fluorescently labelled and lineage traced from a multicolor tracer. The gray color codes for the second harmonic generation (SHG) signal from Collagen 1.…Read article
Mission Impossible Accomplished: Tunable Colors for Non-descanning Detection
Leica Microsystems’ 4Tune detector, the key component of the SP8 DIVE Deep In Vivo Explorer, provides spectrally tunable image recording with non-descanning detection. An innovative solution for…Read article
Laser Beam Shaping for Multicolor Multiphoton Microscopy
Multiphoton Microscopy is one of the current hot topics in life science research. The new Leica TCS SP8 DIVE from Leica Microsystems presents a series of beneficial new innovations, including a freely…Read article
Five Questions Asked: Prof. Dr. Jacco van Rheenen speaks about the most important considerations when imaging deep into mouse tissue
When operating a confocal microscope, or when discussing features and parameters of such a device, we inescapably mention the pinhole and its diameter. This short introductory document is meant to…Read article
BABB Clearing and Imaging for High Resolution Confocal Microscopy
Multipohoton microscopy experiment using Leica TCS SP8 MP and Leica 20x/0.95 NA BABB immersion objective. Understanding kidney microanatomy is key to detecting and identifying early events in kidney…Read article
Clearing of Fixed Tissue: A Review from a Microscopist’s Perspective
Chemical clearing of fixed tissues is becoming a key instrument for the three-dimensional reconstruction of macroscopic tissue portions, including entire organs. Indeed, the growing interest in this…Read article
4Pi-RESOLFT Nanoscopy
Here we apply the 4Pi scheme to RESOLFT nanoscopy using two-photon absorption for the on-switching of fluorescent proteins. We show that in this combination, the lobes are so low that low-light level,…Read article
From Light to Mind: Sensors and Measuring Techniques in Confocal Microscopy
This article outlines the most important sensors used in confocal microscopy. By confocal microscopy, we mean "True Confocal Scanning", i.e. the technique that illuminates and measures one single…Read article
Correlating Intravital Multi-Photon Microscopy to 3D Electron Microscopy of Invading Tumor Cells Using Anatomical Reference Points
Cancer research unsing multiphoton microscopy and 3D electron microscopy. Correlative microscopy combines the advantages of both light and electron microscopy to enable imaging of rare and transient…Read article
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