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Learn & Share: Quantitative Fluorescence

The phenomenon of fluorescence has revolutionized research in biology and medicine. More than 100 years ago, Paul Ehrlich began to develop specific chemical staining methods and the latest development of light-controlled proteins (optogenetics) will surely not mark the end of this progression. A historically continuous trend is the quest for measuring and quantifying data, with or without generation of images as data source. Many techniques have been introduced that are based on fluorescence, but not necessarily require image formation. The laboratory jargon refers to this methods as “F-techniques”: FLIM, FCS , FCCS, FRET, FRAP… to mention just the most important. Read the articles in this topic, to find out more details on these modern methods.

Useful Links

Communities and Web Sources

www.researchgate.net/Social network for scientists

www.lsoft.com/scripts/wl.exe?SL1=CONFOCALMICROSCOPY&H=LISTS.UMN.EDUConfocal Microscopy Mailing List, University of Minnesota

www.ibiology.org/Teaching tools, video lectures on biology and microscopy

bitesizebio.comOnline magazine and community for molecular and cell biology researchers

www.somersault1824.comResource for high-end scientific illustrations, images and animations

Search Engines and Data Bases

www.cellimagelibrary.orgPublic resource database of images, videos, and animations of cells

harvester.fzk.de/harvesterBioinformatic meta search engine for genes and proteins

www.gopubmed.comSearch interface for pubmed

en.wikipedia.org/wiki/List_of_academic_databases_and_search_enginesList of academic databases and search engines

scholar.google.comBeta of Google's search engine for scientific article abstracts

Journals

www.doaj.org/Directory of open access journals

emboj.embopress.org/The EMBO Journal

www.lifescied.orgCBE-Life Sciences Education – an ASCB online journal

www.sciencemag.org/Science

www.nature.com/Nature

www.cell.com/Biweekly publication of exceptional research articles

jcs.biologists.org/Journal of Cell Science

dev.biologists.org/Development

jeb.biologists.org/The Journal of Experimental Biology

dmm.biologists.org/DMM Disease Models & Mechanisms

www.biotechniques.com/International Journal of Life Science Methods

www.opticsinfobase.org/Collection of Journals and Proceedings in Optics and Photonics

spie.org/x576.xmlSPIE - peer-reviewed journals on applied research in optics and photonics

onlinelibrary.wiley.com/journal/10.1002/(ISSN)1864-0648Journal of Biophotonics

www.plosone.org/home.actionInternational, peer-reviewed, open-access, online publication

rspb.royalsocietypublishing.org/Proceedings B - the Royal Society's biological research journal

www.microscopy-analysis.com/International Journal for microscopists

Organizations

www.microscopy.org/Microscopy Society of America

www.eurmicsoc.org/European Microscopy Society

www.rms.org.uk/Royal Microscopical Society

www.ascb.org/ASCB American Society of Cell Biology

www.biologists.com/cob_activities.htmlthe company of biologists

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