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What is Light Sheet Microscopy?

Living specimens, particularly developing embryos, are often sensitive to light exposure. Light sheet microscopy – also referred to as single plane illumination microscopy or SPIM – is a gentle way of imaging sensitive samples or fast biological processes in vivo. The specimen is illuminated only in a single plane at a time and detected from the perpendicular direction. Since there is no out-of-focus excitation, phototoxic effects are reduced to the focal plane. Light sheet imaging has intrinsic optical sectioning. By moving the sample through the light sheet, 3D images of a specimen can be recorded.

Useful Links

Publications on Light Sheet Microscopy

Visualization and targeting of LGR5+ human colon cancer stem cellsShimokawa, M. et al., 2017

Super-Resolution Mapping of Neuronal Circuitry With an Index-Optimized Clearing AgentKe, M.-T. et al., 2016

Light Sheet Microscopy to Measure Protein Dynamics.Rieckher, M., 2016

Further reading on Light Sheet Microscopy, J. et al., 2004, A. H. et al., 1993

Light Sheet Fluorescence Microscopy International Conference 2017 2016 2015 2014

Communities and Web Sources network for scientists tools, video lectures on biology and microscopy

www.bitesizebio.comOnline magazine and community for molecular and cell biology researchers

www.somersault1824.comResource for high-end scientific illustrations, images and animations

Search Engines and Data Bases

www.cellimagelibrary.orgPublic resource database of images, videos, and animations of cells meta search engine for genes and proteins

www.gopubmed.comSearch interface for pubmed of academic databases and search engines of Google's search engine for scientific article abstracts

Journals of open access journals EMBO Journal

www.lifescied.orgCBE-Life Sciences Education – an ASCB online journal publication of exceptional research articles of Cell Science Journal of Experimental Biology Disease Models & Mechanisms
International Journal of Life Science Methods of Journals and Proceedings in Optics and Photonics - peer-reviewed journals on applied research in optics and photonics of Biophotonics, peer-reviewed, open-access, online publication B - the Royal Society's biological research journal Journal for microscopists

Organizations / Institutes Society of America Microscopy Society Microscopical Society

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