Learn & Share: Widefield Microscopy?
Widefield microscopy refers to a basic sample illumination principle in microscopy. Since widefield microscopy permanently illuminates the whole sample, it can be distinguished from confocal microscopy where only one single focal spot is illuminated and recorded at a time. Typically, widefield microscopy utilizes light sources such as halogen, metal halide lamps or LED for sample illumination. Detection is performed through oculars or with the help of a digital camera. Several contrast methods increase widefield microscopy capabilities, starting from Phase Contrast through to Differential Interference Contrast (DIC) or fluorescence, to name but a few. Computer based deconvolution can be applied to increase widefield fluorescence image quality and enable 3D image reconstruction. Moreover, TIRF and GSDIM super-resolution microscopy can be assigned to widefield microscopy.
Image Gallery: THUNDER Imager
To help you answer important scientific questions, THUNDER Imagers eliminate the out-of-focus blur that clouds the view of thick samples when using camera-based fluorescence microscopes. They achieve…Read article
Webinar: Digital Classroom Options
As teachers, you know your big challenge is to catch and keep the students’ attention and the best chance for this is by making the environment interactive. In the case of the Microscopy Classroom, we…Read article
Alzheimer Plaques: fast Visualization in Thick Sections
More than 60% of all diagnosed cases of dementia are attributed to Alzheimer’s disease. Typical of this disease are histological alterations in the brain tissue. So far, there is no cure for this…Read article
How to Develop an Airway Epithelial Cell Model
The present study describes the optimal growth conditions for the proliferation and differentiation of bovine bronchial epithelial cells (BBECs). The cells are grown at an air-liquid interface (ALI)…Read article
THUNDER Technology Note
So far, widefield microscopy was not suitable to image larger volumes, since the contrast of the recorded fluorescence image is reduced by the background (BG) mainly originating from out of focus…Read article
Proline Metabolism is Essential for Trypanosoma brucei brucei Survival in the Tsetse Vector
Bloodsucking insects play a major role in the transmission of pathogens that cause major tropical diseases. Their capacity to transmit these diseases is directly associated with the availability and…Read article
mTORC1 Promotes Proliferation of Immature Schwann Cells and Myelin Growth of Differentiated Schwann Cells
The myelination of axons is essential for neuronal wiring and normal nervous system functions. In the peripheral nervous system, Schwann cells (SCs) form myelin sheaths around axons during nerve…Read article
Effect of curcumin analogs onα-synuclein aggregation and cytotoxicity
Alpha-synuclein (α-Syn) aggregation into oligomers and fibrils is associated with dopaminergic neuron loss occurring in Parkinson’s disease (PD) pathogenesis. Compounds that modulate α-Syn aggregation…Read article
Expression Analysis of Platelet‐derived Growth Factor Receptor Alpha and its Ligands in the Developing Mouse Lung
Activation of the platelet‐derived growth factor receptor‐α (PDGFR α) signaling pathway is critically important during lung alveogenesis, the process in lung development during which alveoli are…Read article
Acute Transcriptional Up-regulation Specific to Osteoblasts/Osteoclasts in Medaka Fish Immediately after Exposure to Microgravity
Bone loss is a serious problem in spaceflight; however, the initial action of microgravity has not been identified. To examine this action, we performed live-imaging of animals during a space mission…Read article
Adipose Stem Cells Display Higher Regenerative Capacities and More Adaptable Electro-Kinetic Properties Compared to Bone Marrow-Derived Mesenchymal Stromal Cells
Adipose stem cells (ASCs) have recently emerged as a more viable source for clinical applications, compared to bone-marrow mesenchymal stromal cells (BM-MSCs) because of their abundance and easy…Read article
Real Time Observation of Neutrophil White Blood Cell Recruitment to Bacterial Infection In Vivo
The zebrafish (Danio rerio) is an emerging vertebrate model organism to study infection. The transparent larva comprises a fully functional innate immune system and enables live imaging of fluorescent…Read article
Introduction to Widefield Microscopy
One of the most basic microscopy techniques is known as ‘Widefield Microscopy’. It is fundamentally any technique in which the entire specimen of interest is exposed to the light source with the…Read article
Chronic Inflammation Under the Microscope
In the course of chronic inflammation certain body areas are recurrently inflamed. This goes along with many human diseases. With the help of widefield light microscopy, the underlying processes can…Read articleImage Gallery: Application Options with Leica DM2500 LED Microscope
The number of applications in microscopy can be as manifold as life itself. Different specimens demand different contrast methods to get a decent result. Unstained cells normally do not show a very…Read article