Learn & Share: Widefield Microscopy?
Widefield microscopy refers to a basic sample illumination principle in microscopy. Since widefield microscopy permanently illuminates the whole sample, it can be distinguished from confocal microscopy where only one single focal spot is illuminated and recorded at a time. Typically, widefield microscopy utilizes light sources such as halogen, metal halide lamps or LED for sample illumination. Detection is performed through oculars or with the help of a digital camera. Several contrast methods increase widefield microscopy capabilities, starting from Phase Contrast through to Differential Interference Contrast (DIC) or fluorescence, to name but a few. Computer based deconvolution can be applied to increase widefield fluorescence image quality and enable 3D image reconstruction. Moreover, TIRF and GSDIM super-resolution microscopy can be assigned to widefield microscopy.
Finding new Scaffolds for Tissue Engineering
Tissue engineers use biomaterials for a variety of applications from drug delivery to supporting the regeneration of damaged or lost tissues to creating in vitro disease models. Scaffold architecture…Read articleThe Power of Pairing Adaptive Deconvolution with Computational Clearing
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Understanding cell functions in normal and tumorous tissue is a key factor in advancing research of potential treatment strategies and understanding why some treatments might fail. Single-cell…Read articlePlant Cell Development and Morphogenesis
The article discusses how tubulin molecules, which make up microtubules, in plant cells can be studied with total internal reflection fluorescence (TIRF) microscopy to better understand tubulin…Read articleStudying Pulmonary Fibrosis
The results shown in this article demonstrate that fibrotic and non-fibrotic regions of collagen present in mouse lung tissue can be distinguished better with polarized light compared to brightfield.…Read articleStudying Autoimmune Disease
This article discusses how autoimmune diseases, like systemic lupus erythematosus (SLE), can be studied more efficiently using thick, 3D kidney tissue specimens visualized with a THUNDER Imager . SLE…Read articleImage Gallery: THUNDER Imager
To help you answer important scientific questions, THUNDER Imagers eliminate the out-of-focus blur that clouds the view of thick samples when using camera-based fluorescence microscopes. They achieve…Read articleFrom Organs to Tissues to Cells: Analyzing 3D Specimens with Widefield Microscopy
Obtaining high-quality data and images from thick 3D samples is challenging using traditional widefield microscopy because of the contribution of out-of-focus light. In this webinar, Falco Krüger…Read articleStudying Human Brain Development and Disease
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Widefield fluorescence microscopy is often used to visualize structures in life science specimens and obtain useful information. With the use of fluorescent proteins or dyes, discrete specimen…Read articleCrystal clear cryo light-microscopy images
This article describes how computational clearing of cryo light microscopy images improves the identification of cellular targets for cryo electron-microscopy.Read articleMicroscopy in Virology
The coronavirus SARS-CoV-2, causing the Covid-19 disease effects our world in all aspects. Research to find immunization and treatment methods, in other words to fight this virus, gained highest…Read articleIL-18 cytokine derived from the enteric nervous system is important for intestinal immunity
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The present study describes the optimal growth conditions for the proliferation and differentiation of bovine bronchial epithelial cells (BBECs). The cells are grown at an air-liquid interface (ALI)…Read articleTHUNDER Technology Note
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Useful Links
Communities and Web Sources
www.researchgate.net/Social network for scientists
www.lsoft.com/scripts/wl.exe?SL1=CONFOCALMICROSCOPY&H=LISTS.UMN.EDUConfocal Microscopy Mailing List, University of Minnesota
www.ibiology.org/Teaching tools, video lectures on biology and microscopy
www.ibiology.org/Teaching tools, video lectures on biology and microscopy
bitesizebio.comOnline magazine and community for molecular and cell biology researchers
www.somersault1824.comResource for high-end scientific illustrations, images and animations
Search Engines and Data Bases
www.cellimagelibrary.orgPublic resource database of images, videos, and animations of cells
harvester.fzk.de/harvesterBioinformatic meta search engine for genes and proteins
www.gopubmed.comSearch interface for pubmed
en.wikipedia.org/wiki/List_of_academic_databases_and_search_enginesList of academic databases and search engines
scholar.google.comBeta of Google's search engine for scientific article abstracts
Journals
www.doaj.org/Directory of open access journals
emboj.embopress.org/The EMBO Journal
www.lifescied.orgCBE-Life Sciences Education – an ASCB online journal
www.sciencemag.org/Science
www.nature.com/Nature
www.cell.com/Biweekly publication of exceptional research articles
jcs.biologists.org/Journal of Cell Science
dev.biologists.org/Development
jeb.biologists.org/The Journal of Experimental Biology
dmm.biologists.org/DMM Disease Models & Mechanisms
www.biotechniques.com/International Journal of Life Science Methods
www.opticsinfobase.org/Collection of Journals and Proceedings in Optics and Photonics
spie.org/x576.xmlSPIE - peer-reviewed journals on applied research in optics and photonics
onlinelibrary.wiley.com/journal/10.1002/(ISSN)1864-0648Journal of Biophotonics
www.plosone.org/home.actionInternational, peer-reviewed, open-access, online publication
rspb.royalsocietypublishing.org/Proceedings B - the Royal Society's biological research journal
www.microscopy-analysis.com/International Journal for microscopists
Organizations
www.microscopy.org/Microscopy Society of America
www.eurmicsoc.org/European Microscopy Society
www.rms.org.uk/Royal Microscopical Society
www.ascb.org/ASCB American Society of Cell Biology
www.biologists.com/cob_activities.htmlthe company of biologists
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