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Two-color SRS image of human pancreatic organoids showing proteins (red) and lipids (green).
Two-color SRS image of human pancreatic organoids showing proteins (red) and lipids (green).
Webinar

Vibrational Spectroscopic Imaging in Cells, Tissues and Model Organisms

With the SP8 CARS SRS System

Here we present a webinar on the emerging applications of Stimulated Raman Scattering Microscopy, covering the following topics:

  • Quantitative spectroscopic imaging of label-free or chemically tagged specimens: From cell and tissue biology to medical research, materials science, and microfabrication
  • Probing 3D cellular model systems (organoids) and tissues for preclinical & translational research in cancer, neurodegenerative diseases, immunology, and digestive disorders
  • Multi-modal confocal nonlinear optical microscopy, combining CARS, SRS, Second-Harmonic Generation, and 2-Photon Fluorescence for advanced tissue-based diagnostics
  • Monitoring tissue penetration of pharmacological and cosmetic compounds

Image: Two-color SRS image of human pancreatic organoids showing proteins (red) and lipids (green). Sample provided by: Till Moreth, Lotta Hof, Francesco Pampaloni, Prof. Ernst Stelzer lab, Buchmann Institute for Molecular Life Sciences, Frankfurt

Two-color SRS image of human pancreatic organoids showing proteins (red) and lipids (green).

Authors

Topics & Tags

Speaker: Dr. Volker Schweikhard

Dr. Volker Schweikhard obtained a Ph.D in Physics at the University of Colorado at Boulder, developing optical manipulation techniques for ultra-cold quantum gases. He then shifted his focus toward research in the Life Sciences, investigating the process of gene transcription at the single-molecule level as a Postdoctoral Fellow at Stanford University, and researching the subcellular coordination of cytoskeletal networks and signaling cascades as a Research Assistant Professor in the Bioengineering Department at Rice University in Houston, TX.

With a background in optics, biophysics and cell biology, it is his passion to develop instruments and procedures to probe biological processes at the (sub-)cellular and tissue levels. At Leica Microsystems, he works with customers, collaboration partners and in-house R&D to drive innovation in nonlinear optical microscopy and multimodal imaging, including coherent Raman scattering microscopy (CARS, SRS), multi-photon fluorescence microscopy, and time-resolved imaging.

Watch the webinar