Vibrational Spectroscopic Imaging in Cells, Tissues and Model Organisms

With the SP8 CARS SRS System

Two-color SRS image of human pancreatic organoids showing proteins (red) and lipids (green). Human_Pancreatic_Organoid_3D_CRS.png

Here we present a webinar on the emerging applications of Stimulated Raman Scattering Microscopy, covering the following topics:

  • Quantitative spectroscopic imaging of label-free or chemically tagged specimens: From cell and tissue biology to medical research, materials science, and microfabrication
  • Probing 3D cellular model systems (organoids) and tissues for preclinical & translational research in cancer, neurodegenerative diseases, immunology, and digestive disorders
  • Multi-modal confocal nonlinear optical microscopy, combining CARS, SRS, Second-Harmonic Generation, and 2-Photon Fluorescence for advanced tissue-based diagnostics
  • Monitoring tissue penetration of pharmacological and cosmetic compounds

Image: Two-color SRS image of human pancreatic organoids showing proteins (red) and lipids (green). Sample provided by: Till Moreth, Lotta Hof, Francesco Pampaloni, Prof. Ernst Stelzer lab, Buchmann Institute for Molecular Life Sciences, Frankfurt

Speaker: Dr. Volker Schweikhard

Dr. Volker Schweikhard obtained a Ph.D in Physics at the University of Colorado at Boulder, developing optical manipulation techniques for ultra-cold quantum gases. He then shifted his focus toward research in the Life Sciences, investigating the process of gene transcription at the single-molecule level as a Postdoctoral Fellow at Stanford University, and researching the subcellular coordination of cytoskeletal networks and signaling cascades as a Research Assistant Professor in the Bioengineering Department at Rice University in Houston, TX.

With a background in optics, biophysics and cell biology, it is his passion to develop instruments and procedures to probe biological processes at the (sub-)cellular and tissue levels. At Leica Microsystems, he works with customers, collaboration partners and in-house R&D to drive innovation in nonlinear optical microscopy and multimodal imaging, including coherent Raman scattering microscopy (CARS, SRS), multi-photon fluorescence microscopy, and time-resolved imaging.

Watch the webinar

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