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Video Talk by Ernst Stelzer: Light Sheet Sectioning

This talk discusses the technique of light sheet microscopy, also known as selective plane illumination (SPIM). This uses two objectives, one to illuminate the sample and a second to image it and allows long-term 3D imaging of thick specimens like developing embryos with minimal photobleaching and phototoxicity.


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YouTube video by iBioEducation, November 2013

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iBiology ( is a collection of open-access online videos by scientists about research and discoveries that cover a wide variety of life science subjects. iBiology has produced over 80 videos by the world's leading scientists including seminars about research in fields from ecology to biophysics, a 65-video course on light microscopy, and over 90 short talks on topics such as professional development, careers, science and society, and education. iBiology is funded by NSF, NIGMS, and HHMI, and supported by the ASCB and UCSF.