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Video Talk by Jeff Lichtman: Point Spread Function

An infinitesimally small point appears in the microscope as a spot with a certain size, blurred in the z-direction and with concentric rings around it. This "point spread function" reveals many of the optical properties of your microscope. This lecture explains why and how the microscope images a point as a point spread function.

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YouTube video by iBioEducation, November 2013

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iBiology (www.ibiology.org) is a collection of open-access online videos by scientists about research and discoveries that cover a wide variety of life science subjects. iBiology has produced over 80 videos by the world's leading scientists including seminars about research in fields from ecology to biophysics, a 65-video course on light microscopy, and over 90 short talks on topics such as professional development, careers, science and society, and education. iBiology is funded by NSF, NIGMS, and HHMI, and supported by the ASCB and UCSF.