Video Talk on Super-Resolution: Overview and Stimulated Emission Depletion (STED) Microscopy
Historically, light microscopy has been limited in its ability to resolve closely spaced objects, with the best microscopes only able to resolve objects separated by 200 nm or more. This limit is known as the diffraction limit. In the last twenty years, a number of techniques have been developed that allow resolution beyond the diffraction limit. Here, iBiology presents Stefan Hell, who invented many of these techniques, gives an introduction to these super-resolution microscopy techniques, and a detailed discussion of two such techniques: STED (STimulated Emission Depletion) and RESOLFT (REversible Saturable OpticaLFluorescence Transitions).