Video Talk on Super-Resolution: Overview and Stimulated Emission Depletion (STED) Microscopy

Historically, light microscopy has been limited in its ability to resolve closely spaced objects, with the best microscopes only able to resolve objects separated by 200 nm or more. This limit is known as the diffraction limit. In the last twenty years, a number of techniques have been developed that allow resolution beyond the diffraction limit. Here, iBiology presents Stefan Hell, who invented many of these techniques, gives an introduction to these super-resolution microscopy techniques, and a detailed discussion of two such techniques: STED (STimulated Emission Depletion) and RESOLFT (REversible Saturable OpticaLFluorescence Transitions).



YouTube video by iBioEducation, November 2013

About iBiology

iBiology ( is a collection of open-access online videos by scientists about research and discoveries that cover a wide variety of life science subjects. iBiology has produced over 80 videos by the world's leading scientists including seminars about research in fields from ecology to biophysics, a 65-video course on light microscopy, and over 90 short talks on topics such as professional development, careers, science and society, and education. iBiology is funded by NSF, NIGMS, and HHMI, and supported by the ASCB and UCSF.

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