Fixation and Dehydration
3% Glutaraldehyde in 0.1M Sodium Phosphate Buffer, pH 7.0 | overnight |
0.1M Sodium Phosphate Buffer, pH 7.0 | 3x10 min. |
Ethanol series: 30%, 50%, 70%, 80%, 90%, 95%, 100% | 2x10 min. |
Leica EM CPD300 auto program


Coating
Gold: 15-20 nm