Introduction
Species: Wall Cress (Arabidopsis thaliana)
Critical point drying of wall cress pod using the Leica EM CPD300 with subsequent gold coating and SEM analysis.
Procedure
Sample Holder:
Samples were inserted into the 22 mm cover slip holder.
Fixation and Dehydration
| 3% Glutaraldehyde in 0.1M Sodium Phosphate Buffer, pH 7.0 | overnight |
| 0.1M Sodium Phosphate Buffer, pH 7.0 | 3x10 min. |
| Ethanol series: 30%, 50%, 70%, 80%, 90%, 95%, 100% | 2x10 min. |
Leica EM CPD300 auto program
Coating
Gold: 15-20 nm
