Wall Cress Pod Protocol - Critical Point Drying of Arabidopsis thaliana for SEM

LiYu, Chen

October 25, 2016 Article

Wall Cress Pod Protocol - Critical Point Drying of Arabidopsis thaliana for SEM

Application Note for Leica EM CPD300 - Life Science Research

Introduction
Species: Wall Cress (Arabidopsis thaliana)
Critical point drying of wall cress pod using the Leica EM CPD300 with subsequent gold coating and SEM analysis.

Procedure
Sample Holder:
Samples were inserted into the 22 mm cover slip holder.

Authors

Chen LiYu, Dr.

Chinese Academy of Sciences, Institute of Genetics and Developmental Biology, Beijing, China

Topics & Tags

EM Sample Preparation

Table of Content

Fixation and Dehydration

3% Glutaraldehyde in 0.1M Sodium Phosphate Buffer, pH 7.0	overnight
0.1M Sodium Phosphate Buffer, pH 7.0	3x10 min.
Ethanol series: 30%, 50%, 70%, 80%, 90%, 95%, 100%	2x10 min.

Leica EM CPD300 auto program