Leica EM CPD300 auto Program:
Coating:
Gold: 10-20 nm
Water flea
Water Flea Protocol
Application Note for Leica EM CPD300 - Life Science Research
Introduction:
Critical point drying of water flea with subsequent gold coating and SEM-Analysis to detect finde surface structures.
Procedure:
Sample Holder:
Sample was inserted into a filter disc
(Pore size: 16 - 40 μm).
Filter disc was places into the cover slip holder 18 mm.
Fixation and Dehydration:
| 2.5% Glutaraldehyde in 0.1M Sodium Cacodylate Buffer, 2% Sucrose, pH 7.3 | 1x 18 h |
| 0.1 M Sodium Cacodylate Buffer, 2 % Sucrose, pH 7.3 | 3x 10 min. |
| 0.1% OsO4, in 0.1M Sodium Cacodylate Buffer, 2% Sucrose, pH 7.3 | 1x 1 h |
| 0.1 M Sodium Cacodylate Buffer, 2 % Sucrose, pH 7.3 | 3x 10 min. |
| Ethanol 30%, 50%, 70%, 80%, 90%, 96%, 100% | 2x 10 min. |
| 100% Acetone, 1% Dimethoxypropane | 2x 30 min. |
