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Webinar: Challenges in Live Cell Imaging

Live cell imaging is one tool in the cell biologist's tool box. There are, however, a variety of technologies and methods to achieve this. In this webinar, three practicing researchers, Edward Hinchcliffe, PhD, Matthew Lord, PhD, and Dr. Andrew York, discuss:

  • Their use of live cell imaging
  • How it has helped them achieve publishable results.


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Matthew Lord, Ph.D.

Assistant Professor, Department of Molecular Physiology and Biophysics, University of Vermont:

Total internal reflection fluorescence (TIRF) microscopy provides a powerful approach with which to study the dynamic behavior of proteins in cells at high spatial resolution. In addition, TIRF microscopy lends itself to in vitro studies where the activity of single protein molecules can be monitored in real time. During this webinar, Matthew Lord, PhD, will demonstrate the utility of this technique by showing how it has advanced our understanding of intracellular transport by an actin filament-based motor protein called myosin-V.



Edward Hinchcliffe, Ph.D.

Associate Professor, Cellular Dynamics, The Hormel Institute, University of Minnesota

Edward Hinchcliffe, Ph.D. will discuss live cell imaging of GFP-expressing cells using spinning disk confocal microscopy. The Hinchcliffe lab uses cultured mammalian cells and cytoplasmic extracts generated from Xenopus frogs to examine the basic control mechanisms underlying centrosome duplication – using advanced imaging techniques to address fundamental questions in cell biology.



Dr. Andrew York

Research Fellow on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering:

Dr. Andrew York will discuss three-dimensional (3D) super-resolution in live multicellular organisms using structured illumination microscopy (SIM). Sparse multifocal illumination patterns generated by a digital micromirror device (DMD) allowed the researchers to physically reject out-of-focus light, enabling 3D subdiffractive imaging in samples eightfold thicker than had been previously imaged with SIM.




Robert Fee