The beauty of cells, magnified and resolved by light microscopy, has been fascinating investigators since the 19th century. Today, functional research in living cells is often a prerequisite for biological studies. And keeping the cells close to the necessary conditions whilst under microscopical observation is key.
In this webinar, Dr Christoph Bauer will share his experiences of live cell imaging and talk about how the microscopy challenges, such as optical aberrations at 37 °C to or focus drift in long-term experiments, can be addressed. This webinar will also talk about the challenge of overcoming the diffraction limit. Several approaches to overcome this limitation have been developed and super-resolution microscopy has proven an extremely valuable tool.
The gated STED technology realized on the confocal platforms Leica TCS SP5 and SP8 drives live cell super-resolution to the next level. Details smaller than 50 nm can be revealed and super-resolved dynamic studies performed with common fluorescence proteins. Resolution can be further increased by with localization microscopy techniques, such as