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Webinar: See More Than Just Your Image

Resolve Structural Information Not Previously Visible

Despite the emergence of new imaging methods in recent years, true 3D resolution is still achieved by Confocal Laser Scanning Microscopy (CLSM). Through a combination of novel, extremely fast scanning methods with high sensitivity, low noise detectors and simultaneous multi-spectral data acquisition, Leica’s confocal imaging has now been extended to the point that previously inaccessible dynamic and spectral ranges became accessible.

They can push these limits further! The identification of interfering signals via intelligent models and the correlation of individual photons with their original location via the process of deconvolution. LIGHTNING, a completely new system integrated module, is a fully automated and highly reliable image extraction information system, completely independent of manual user input.


Dr. Juergen Reymann

Dr. Jayshan Carpen

Topics & Tags

Table of Content

Key Learnings

  • How to resolve structural information not previously accessible in your data
  • How LIGHTNING Image Information Extraction can push the limits in the spatial and temporal domain
  • Microscopy image formation and limitations due to diffraction, background and noise
  • How LIGHTNING delivers great results – fully automated and near real-time

This unique development reaches resolutions far below the theoretical diffraction limit, revealing image information which, although spatially and temporally structurally present in the image data, were not previously visible due to diffraction and noise.