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Webinar: Super-Resolution

Light Microscopy for the 21st Century

In 1873, Ernst Abbe developed a theory that defined the limit of resolution of the light microscope. Following suit from astronomy, Abbe defined resolution as the ability to resolve, as separate, two point sources of light. The Abbe limit of 200–300 nm is based upon the ability of the light microscope to collect only a subset of spatial frequencies and the physiological properties of the human eye. Starting in the 1980’s with the advent of video, now digital microscopy, microscopists began to push these limits, based upon the linear response of video detectors. True super-resolution techniques, such as scanning nearfield microscopy, which collects the higher spatial frequencies resident in the evanescent wave were developed. While pushing the resolution limit an order of magnitude to ~ 20 nm these techniques had limited applicability to the demands of modern cell biology. Starting in the 1990’s, a whole new spectrum of super-resolution techniques have ushered in a renaissance in biological microscopy. These techniques employ single molecule detection approaches and the non-linear absorption properties of fluorescent materials to restructure the microscope’s point spread function. In this webinar, leaders in the field of super-resolution will explain the physical bases of these new approaches and describe how they will redefine our understanding of cell and molecular biology in the 21st century.

Who should attend:
This webinar is essential for all life-scientists who use or are considering applying super-resolution microscopy in their research.


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In association with


Dr. Stefan Hell

Director, Department of NanoBiophotonics, Director Analytical and Quantitative Light Microscopy Course, University of Göttingen

Dr. Hari Shroff

Chief and Investigator, Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA

Dr. Paul R. Selvin

Professor of Physics, University of Illinois at Urbana-Champaign, Urbana, IL, USA