Leica EM CPD300 auto program
Coating
Gold: 15-20 nm
Wrinkled Giant Hyssop Leaf Protocol
Application Note for Leica EM CPD300 - Life Science Research
Introduction
Species: Wrinkled Giant Hyssop (Agastache rugosa)
Critical point drying of wrinkled giant hyssop leaf with subsequent gold coating and SEM analysis
Procedure
Sample Holder: Samples were inserted into the 22 mm cover slip holder
Fixation and Dehydration
| 2.5% Glutaraldehyde in 0.1M Sodium Phosphate Buffer, pH 7.2 | 14 h |
| 0.1M Sodium Phosphate Buffer, pH 7.2 | 3x10 min. |
| Acetone series: 30%, 50%, 70%, 80%, 90%, 95%, 100% | 2x10 min. |
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