Wrinkled Giant Hyssop Leaf Protocol

JianSheng, Guo

August 11, 2016 Story

Wrinkled Giant Hyssop Leaf Protocol

Application Note for Leica EM CPD300 - Life Science Research

Introduction
Species: Wrinkled Giant Hyssop (Agastache rugosa)
Critical point drying of wrinkled giant hyssop leaf with subsequent gold coating and SEM analysis

Procedure
Sample Holder: Samples were inserted into the 22 mm cover slip holder

Fixation and Dehydration

2.5% Glutaraldehyde in 0.1M Sodium Phosphate Buffer, pH 7.2	14 h
0.1M Sodium Phosphate Buffer, pH 7.2	3x10 min.
Acetone series: 30%, 50%, 70%, 80%, 90%, 95%, 100%	2x10 min.