Fluorescence Lifetime Imaging

Fluorescence lifetime imaging microscopy (FLIM) is an imaging technique that makes use of the inherent properties of fluorescent dyes. Aside from having characteristic emission spectra, each fluorescent molecule has a characteristic lifetime that reflects how long the fluorophore spends in the excited state before emitting a photon. Lifetime analysis provides you with information in addition to your standard fluorescence intensity measurements.

Historically, lifetime imaging has been a slow, complex, and specialized technique. Its use was limited to experienced microscopists or physicists. Leica Microsystems is at the cutting edge of today’s fluorescence lifetime imaging advances. Our systems make lifetime imaging faster and easier to use than ever before, binging its advantages to every day confocal imaging experiments.

Simply get in touch!

Our experts on solutions for FLIM are happy to help you with their advice.

Boost confocal imaging with fluorescence lifetime information

Fluorescence lifetime imaging provides additional information that can help you improve the quality of your confocal imaging. It can be particularly useful to discriminate fluorescence probes that have overlapping fluorescent emission spectra, or to eliminate unwanted background fluorescent signals.

FLIM is a crucial application for measuring and quantifying imaging data. As lifetime information is independent of fluorophore concentration, it is extremely well suited for functional imaging. Functional imaging goes beyond the traditional recording of the location and concentration of molecular species and enables further investigation of molecular function, interactions, and their environment. 

Additionally, FLIM enables novel imaging applications, such as the use of biosensors to study the cell’s microenvironment or imaging based on the intrinsic fluorescent properties of your sample.

Learn more about FLIM and its imaging applications with the SP8 FALCON system by referring to this application note.

The lifetime contrast of sepal of silverberry allows to separate different structure within the sample. The intensity image (yellow image) doesn't show any specific structure while the FastFLIM (rainbow scale image) and the lifetime fitted images (RGB, three component image) give insigth into the different part of the sample. The end of the video shows the three structures determined by the lifetime fit split into three channels (RGB).

Setting the standard for lifetime imaging

With the release of the fast and fully integrated lifetime imaging technology – the SP8 FALCON – Leica Microsystems set the benchmark for the future of lifetime imaging. Leica Microsystems received the prestigious R&D 100 Award for the SP8 FALCON system. This award recognizes the solution’s innovative approach to lifetime imaging.

Leica Microsystems solutions for FLIM enable you to harness the power of fluorescence lifetime to extract a new dimension of information from every sample with your confocal experiment. Investigate cellular physiology and explore cellular dynamics with FALCON. Get immediate access to functional information such as metabolic status, pH and ion concentration with the potential of Tausense in STELLARIS.

With our simplified, integrated platforms, you can gain new perspectives on your experiments, allowing you to harness new answers to your experimental questions.

FLIM Products

Filter by Area of Application
STELLARIS - Confocal Microscope Platform

STELLARIS

With the STELLARIS confocal platform, we have re-imagined confocal microscopy to get you closer to the truth.

Related Articles

Read our latest articles about Fluorescence Lifetime Imaging

The knowledge portal of Leica Microsystems offers scientific research and teaching material on the subjects of microscopy. The content is designed to support beginners, experienced practitioners and scientists alike in their everyday work and experiments.

More Articles

Fluorescence Lifetime-based Imaging Gallery

Confocal microscopy relies on the effective excitation of fluorescence probes and the efficient collection of photons emitted from the fluorescence process. One aspect of fluorescence is the emission…
Read article

Phasor FLIM Detects Metabolic Differences

The maintenance of normal blood glucose levels is defective in diabetes caused by dysfunction of the alpha and beta cells in islets. To understand these regularities, we used multiphoton phasor-FLIM
Read article

Accessing the Metabolic Information of Stem Cells

Cancer often comes with changes in the metabolism of cells. Monitoring the metabolic state using fluorescence lifetime imaging microscopy (FLIM) is a good way to assess these changes. In some cases,…
Read article

How to Quantify Changes in the Metabolic Status of Single Cells

Metabolic imaging based on fluorescence lifetime provides insights into the metabolic dynamics of cells, but its use has been limited as expertise in advanced microscopy techniques was needed. Now,…
Read article

Label-free Differentiation of Microplastics

Phasor analysis for fluorescence lifetime imaging microscopy (FLIM) is a powerful and fitting-free tool that provides a 2D graphical assessment of pixel-accurate lifetime distributions in a sample…
Read article

Adding Dimensions to Multiplex Molecular Imaging

Molecular imaging of living specimens offers a means to draw upon the growing body of high-throughput molecular data to better understand the underlying cellular and molecular mechanisms of complex…
Read article

How FLIM Microscopy helps to detect microplastic pollution

The use of autofluorescence in biological samples is a widely used method to gain detailed knowledge about systems or organisms. This property is not only found in biological systems, but also…
Read article

Advanced Quantitative Fluorescence Microscopy to Probe the Molecular Dynamics of Viral Entry

Viral entry into the host cell requires the coordination of many cellular and viral proteins in a precise order. Modern microscopy techniques are now allowing researchers to investigate these…
Read article

Time-resolved STED microscopy

Introduced more than 30 years ago, stimulated emission depletion (STED) microscopy has raised to a standard and widely used method for imaging in the life sciences. Thanks to continuous technological…
Read article

Imaging Intracellular Temperature using Fluorescence Lifetime Imaging Microscopy (FLIM)

This video shows the presentation of Dr. Kohki Okabe given at the 9th Congress of the Federation of Asian and Oceanian Physiological Society (FAOPS) held in Kobe, Japan on 30 March 2019. Dr. Okabe…
Read article

SP8 FALCON: a novel concept in fluorescence lifetime imaging enabling video-rate confocal FLIM

SP8 FALCON (FAst Lifetime CONtrast) is a fast and completely integrated fluorescence lifetime imaging microscopy (FLIM) confocal platform. SP8 FALCON delivers video-rate FLIM with pixel-by-pixel…
Read article

Development of fluorescence lifetime imaging microscopy (FLIM) and its relevance for functional imaging

Prof. Ammasi Periasamy, Director, Keck Center for Cellular Imaging, University of Virginia, was interviewed by Dr. Giulia Ossato, Product Manager functional imaging, during Leica Microsystems Meets…
Read article

How FRET/FLIM Biosensors and Lifetime Imaging Helped Identify the Importance of Zinc for Human B-Cell Development

In her latest studies, Dr. Mukta Deobagkar-Lele investigated the role of ZIP7, a zinc transporter protein, in modulating B cell activity and its effects on human immunity. During an interview with Dr.…
Read article

Single-residue bioorthogonal labeling of G protein-coupled receptors (GPCRs) in live cells for quantitative analysis

To perform quantitative studies of membrane bound G protein-coupled receptors (GPCRs) with high-performance microscopy, the proteins must first be labeled with bright and photostable dyes. The…
Read article

Phasor Analysis for FLIM (Fluorescence Lifetime Imaging Microscopy)

The Phasor analysis approach to analyze fluorescence lifetime does not require any fitting. Phasor FLIM (fluorescence lifetime imaging microscopy) provides a 2D graphical view of lifetime…
Read article
primary murine pre B cells expressing the eCALWY-6 Zn2+

Researchers use Functional Imaging to Elucidate the Role of Cellular Zn²⁺ in Human Immunity

An international consortium of researchers has found a new mutation in a gene harboring a reticulum-to-cytoplasm zinc transporter. The gene is called ZIP7 and it is responsible for the early onset of…
Read article

SP8 DIVE Multiphoton Microscope Image Gallery

Today’s life science research focusses on complex biological processes, such as the causes of cancer and other human diseases. A deep look into tissues and living specimens is vital to understanding…
Read article

Precise and lightning-fast spectral Fluorescence Lifetime Imaging at video rate integrated in a high-end confocal microscope

Förster (Fluorescence) Resonance Energy Transfer (FRET) has become a powerful tool to study protein-protein interactions and signal transduction in living cells. FRET is commonly read out either by…
Read article

Interested to know more?

Talk to our experts. We are happy to answer all your questions and concerns.

Contact Us

Do you prefer personal consulting?

  • Leica Microsystems Inc.
    1700 Leider Lane
    Buffalo Grove, IL 60089 United States
    Office Phone : +1 800 248 0123
    Service Phone : 1 800 248 0223
    Fax : +1 847-236-3009

You will find a more detailed list of local contacts here.