Leica Science Lab - Topic : Fluorescence Microscopy https://www.leica-microsystems.com//solutions/life-science/fluorescence/?tx_leicaacademy_pi2%5Baction%5D=listPagesForTopic&tx_leicaacademy_pi2%5Bcontroller%5D=Topic&tx_leicaacademy_pi2%5Btopic%5D=21&cHash=02519c6e89d6e0bb8fbe5e5b6db54e80 Article tagged with Fluorescence Microscopy en-US https://www.leica-microsystems.com/48987 THUNDER Imaging Fluorescence Microscopy Widefield Microscopy Studying Cell Division Cell division is a biological process during which all cellular components must be distributed among the daughter cells. The division process requires firm coordination for success. Microscopy is utilized to observe this procedure in living cells. https://www.leica-microsystems.com/science-lab/studying-cell-division/ Tue, 09 Mar 2021 13:25:00 +0000 Ph.D. Javier Encinar del Dedo, Ph.D. Elena Rebollo, Dr. Christoph Greb, PhD James DeRose https://www.leica-microsystems.com/71300 THUNDER Imaging Fluorescence Microscopy Widefield Microscopy Viral Infections – Studying Influenza-host Interactions in 3D Specimens Stefan Finke studies virus-host interactions. It turns out that the ways the virus and host interact are different when comparing results from classical laboratory monolayer cell lines and “close to in vivo” infection models. https://www.leica-microsystems.com/science-lab/viral-infections-studying-influenza-host-interactions-in-3d-specimens/ Thu, 04 Mar 2021 08:32:00 +0000 Dr. Christoph Greb https://www.leica-microsystems.com/48471 THUNDER Imaging Fluorescence Microscopy Widefield Microscopy Finding new Scaffolds for Tissue Engineering Tissue engineers use biomaterials for a variety of applications from drug delivery to supporting the regeneration of damaged or lost tissues to creating in vitro disease models. Scaffold architecture can be tailored to specific tissue engineering applications. Characterizing scaffold morphology and porosity through imaging is crucial to the fabrication of modular biomaterials. Widefield microscopy can reach its limits when used for tissue engineering due to the thickness and optical characteristics of the tissue. THUNDER Imagers equipped with the Computational Clearing technology offer the advantages of widefield microscopy, but overcome the out-of-focus blur or “haze” typical when imaging thick, 3D specimens. https://www.leica-microsystems.com/finding-new-scaffolds-for-tissue-engineering/ Wed, 17 Feb 2021 09:26:00 +0000 Mollie Smoak, Dr. Christoph Greb, PhD James DeRose https://www.leica-microsystems.com/48638 THUNDER Imaging Widefield Microscopy Fluorescence Microscopy The Power of Pairing Adaptive Deconvolution with Computational Clearing Deconvolution is a computational method used to restore the image of the object that is corrupted by the point spread function (PSF) along with sources of noise. In this technical brief, learn how the deconvolution algorithm offered by Leica Microsystems allows you to overcome losses in image resolution and contrast in widefield (WF) fluorescence microscopy due to the wave nature of light and the diffraction of light by optical elements. Explore the methods of user-controlled or automated deconvolution to see and resolve more structural detail. https://www.leica-microsystems.com/the-power-of-pairing-adaptive-deconvolution-with-computational-clearing/ Tue, 16 Feb 2021 11:22:00 +0000 PhD Vikram Kohli, PhD James M. Marr, Dr. Oliver Schlicker, Dr. Levi Felts https://www.leica-microsystems.com/48615 THUNDER Imaging Fluorescence Microscopy Widefield Microscopy Improvement of Imaging Techniques to Understand Organelle Membrane Cell Dynamics Understanding cell functions in normal and tumorous tissue is a key factor in advancing research of potential treatment strategies and understanding why some treatments might fail. Single-cell analysis is crucial in biomedical research to unravel which cellular and molecular pathways are altered in complex diseases such as cancer. https://www.leica-microsystems.com/improvement-of-imaging-techniques-to-understand-organelle-membrane-cell-dynamics/ Mon, 15 Feb 2021 12:11:00 +0000 Corporate Communications https://www.leica-microsystems.com/46588 Fluorescence Microscopy Widefield Microscopy Plant Cell Development and Morphogenesis The article discusses how tubulin molecules, which make up microtubules, in plant cells can be studied with total internal reflection fluorescence (TIRF) microscopy to better understand tubulin dynamics and cell development and morphogenesis. To visualize tubulin molecules in microtubules, an imaging solution is needed that allows them to be easily resolved near the plant cell surface. Conventional widefield microscopy detects too much out-of-focus fluorescence signal leading to reduced resolution. TIRF microscopy minimizes out-of-focus fluorescence and enables the tubulin to be clearly resolved, making TIRF a useful tool for studying molecular dynamics. https://www.leica-microsystems.com/plant-cell-development-and-morphogenesis/ Thu, 28 Jan 2021 10:59:00 +0000 PhD Olga Davydenko, PhD James DeRose https://www.leica-microsystems.com/45747 THUNDER Imaging Fluorescence Microscopy Live-Cell Imaging Widefield Microscopy Studying Autoimmune Disease This article discusses how autoimmune diseases, like systemic lupus erythematosus (SLE), can be studied more efficiently using thick, 3D kidney tissue specimens visualized with a THUNDER Imager . SLE can lead to lupus nephritis (LN) when blood vessels in the kidney become inflamed. If left untreated, eventually kidney failure could be the result. A better understanding of the initiation of LN is important for the developing treatments. This goal necessitates the study of 3D kidney specimens. https://www.leica-microsystems.com/studying-autoimmune-disease/ Wed, 27 Jan 2021 09:37:00 +0000 PhD Vikram Kohli, PhD Ameet Chimote, PhD James DeRose https://www.leica-microsystems.com/24935 THUNDER Imaging Fluorescence Microscopy Live-Cell Imaging Widefield Microscopy Stereo Microscopy Image Gallery: THUNDER Imager To help you answer important scientific questions, THUNDER Imagers eliminate the out-of-focus blur that clouds the view of thick samples when using camera-based fluorescence microscopes. They achieve this using Computational Clearing our new opto-digital technology. The result is high-speed, high-quality imaging of a large diversity of three-dimensional samples, including model organisms, tissue sections, and 3D cell cultures. Take a look at these images to see how THUNDER Imagers are already helping researchers to reveal the finest structural details even deep within a sample. Once you have seen the haze-free image quality achieved with THUNDER Imagers, find out more on our product page. Learn how THUNDER Imagers combine Computational Clearing with the speed, fluorescence-signal sensitivity, and ease-of-use of widefield microscopes so you can decode 3D biology in real-time. https://www.leica-microsystems.com/science-lab/galleries/image-gallery-thunder-imager/ Wed, 20 Jan 2021 06:56:00 +0000 https://www.leica-microsystems.com/44724 Confocal Microscopy Fluorescence Microscopy A New World of Confocal Applications with the Next Generation White Light Lasers As biological questions get more complex, there is an increasing need to study multiple events simultaneously in the same specimen. When preparing the specimen for imaging experiments, this need is translated in having to use multiple fluorescent labels. Unfortunately, the visualization of multiple events with confocal microscopy is limited by the number of fluorescent probes that can be simultaneously imaged without incurring bleed-through artifacts due to significant spectral overlap. Using a confocal microscope with an integrated White Light Laser (WLL) enables tuning excitation and detection to perfectly match the spectral profile of the dyes in your sample, giving you the flexibility to perform multicolor experiments while obtaining clear, sharp images. https://www.leica-microsystems.com/science-lab/a-new-world-of-confocal-applications-with-the-next-generation-white-light-lasers/ Thu, 03 Dec 2020 14:20:00 +0000 https://www.leica-microsystems.com/44826 THUNDER Imaging Fluorescence Microscopy Widefield Microscopy From Organs to Tissues to Cells: Analyzing 3D Specimens with Widefield Microscopy Obtaining high-quality data and images from thick 3D samples is challenging using traditional widefield microscopy because of the contribution of out-of-focus light. In this webinar, Falco Krüger shows how THUNDER Imagers with computational clearing makes this possible. https://www.leica-microsystems.com/science-lab/from-organs-to-tissues-to-cells-analyzing-3d-specimens-with-widefield-microscopy/ Thu, 26 Nov 2020 12:12:00 +0000