Laser Microdissection LMD
Laser Microdissection, also known as LMD or LCM (Laser Capture Microdissection), is a contact- and contamination-free method for isolating specific single cells or entire areas of tissue from a wide variety of tissue samples. The thickness, texture and preparation technique of the original tissue are relatively unimportant. The dissectate is then available for further molecular biological methods such as PCR, real-time PCR, proteomics and other analytical techniques. Laser microdissection is now used in a large number of research fields, e.g. neurology, cancer research, plant analysis, forensics or climate research. The method is meanwhile also applied for manipulation of cell cultures or for microengraving of coverslips.
Our LMD Systems are perfect tools to optimize DNA-Workflows (Genomics), RNA-Workflows (Transcriptomics) and Proteomic-Workflows as they allow to precisely define and collect pure starting material for analysis under visual control.
Please contact us if you would like to have personal expert advice on our microscopy solutions for laser microdissection.
Leica Laser Capture Microdissection Products 2
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Leica LMD6 & LMD7
Laser Microdissection enables users to isolate specific single cells or entire areas of tissue.
Leica LMD Software
Laser Microdissection
Quantitative One-Step Protocol to Detect Transcripts in Laser Microdissected Samples
Arbuscular mycorrhizal (AM) fungi are an essential feature of the biology and ecology of most terrestrial plants and, as biofertilizers, AM fungi are an emerging issue in many projects focused on…Read article
Stem Cells in the Aging Process – the Signal Protein p53 as "Guardian of the Genome"
The physiological process of aging is an elementary part of life. The processes responsible for the aging process and the effect they have is one of the key focuses of biomedical research. Dr. Yvonne…Read article
K-ATP Channels in Dopamine Substantia Nigra Neurons Control Bursting and Novelty-induced Exploration
Phasic activation of the dopamine (DA) midbrain system in response to unexpected reward or novelty is critical for adaptive behavioral strategies. This activation of DA midbrain neurons occurs via a…Read article
Comparison of the RNA Quality of Native Tissue Samples with Tissue Samples after UV Laser Microdissection
Due to its instability, RNA is generally more difficult to work with than DNA. RNA does not have the stability of the DNA double helix. To obtain the best results when dealing with RNA it is essential…Read article
Region-Specific Gene Expression in Adult Mouse CNS Tissues
Different areas of the Central Nervous System (CNS) display a specific and selective gene expression profile. Here, we used the Laser Microdissection system Leica LMD6500 to study region-specific mRNA…Read article
TED Talk of Allan Jones: Mapping the Human Brain
How can we begin to understand the complexity of the human brain and the way it works? The same way we begin to understand a city: by making a map. In this talk, Allan Jones shows how his team is…Read article
Cell Cultures and Laser Microdissection
Many of the discoveries that are now being made in cell division and differentiation, the relationships between single cells and cell organelles, treatments of cells with pharmaceutic substances, etc.…Read article
An Introduction to Laser Microdissection
Laser microdissection is a highly selective process for preparing samples for DNA, RNA and protein analysis. It is a microscope-controlled manipulation technique for the precise separation of samples…Read article
Applications of Laser Microdissection
Laser microdissection and laser micromanipulation are suitable for gaining a differentiated insight into the function of genes and proteins, and are used for a wide range of applications in…Read article
Users Report on the Relevance of Laser Microdissection for Their Research Results
Laser dissection is used in a large number of research fields, e.g. neurology, cancer research, plant analysis. Here, user report on the research results they have attained by using laser…Read article
The Mitochondrial Hypothesis of Ageing
Why do we grow old? Research scientists have been looking for an answer to this question for many years – particularly against the background of the increase in neurodegenerative diseases among older…Read article
Useful Links
LMD Software
Video Tutorials on Leica LMD Software
Publication List
LMD reference list for major application fields in life science research
Communities and Web Sources
www.researchgate.net/ Social network for scientists
bitesizebio.com Online magazine and community for molecular and cell biology researchers
www.somersault1824.com Resource for high-end scientific illustrations, images and animations
Search Engines and Data Bases
www.cellimagelibrary.org Public resource database of images, videos, and animations of cells
harvester.fzk.de/harvester Bioinformatic meta search engine for genes and proteins
www.gopubmed.com Search interface for pubmed
en.wikipedia.org/wiki/List_of_academic_databases_and_search_engines List of academic databases and search engines
scholar.google.com Beta of Google's search engine for scientific article abstracts
Journals
www.doaj.org/ Directory of open access journals
emboj.embopress.org/ The EMBO Journal
www.lifescied.org CBE-Life Sciences Education – an ASCB online journal
www.sciencemag.org/ Science
www.nature.com/ Nature
www.cell.com/ Biweekly publication of exceptional research articles
jcs.biologists.org/ Journal of Cell Science
dev.biologists.org/ Development
jeb.biologists.org/ The Journal of Experimental Biology
dmm.biologists.org/ DMM Disease Models & Mechanisms
www.biotechniques.com/ International Journal of Life Science Methods
www.opticsinfobase.org/ Collection of Journals and Proceedings in Optics and Photonics
spie.org/x576.xml SPIE - peer-reviewed journals on applied research in optics and photonics
onlinelibrary.wiley.com/journal/10.1002/(ISSN)1864-0648 Journal of Biophotonics
www.plosone.org/home.action International, peer-reviewed, open-access, online publication
rspb.royalsocietypublishing.org/ Proceedings B - the Royal Society's biological research journal
www.microscopy-analysis.com/ International Journal for microscopists
Organizations
www.microscopy.org/ Microscopy Society of America
www.eurmicsoc.org/ European Microscopy Society
www.rms.org.uk/ Royal Microscopical Society
www.ascb.org/ ASCB American Society of Cell Biology
www.biologists.com/cob_activities.html The company of biologists
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