Laser Microdissection, also known as LMD or LCM (Laser Capture Microdissection), is a contact- and contamination-free method for isolating specific single cells or entire areas of tissue from a wide variety of tissue samples. The thickness, texture and preparation technique of the original tissue are relatively unimportant. The dissectate is then available for further molecular biological methods such as PCR, real-time PCR, proteomics and other analytical techniques. Laser microdissection is now used in a large number of research fields, e.g. neurology, cancer research, plant analysis, forensics or climate research. The method is meanwhile also applied for manipulation of cell cultures or for microengraving of coverslips.
Our LMD Systems are perfect tools to optimize DNA-Workflows (Genomics), RNA-Workflows (Transcriptomics) and Proteomic-Workflows as they allow to precisely define and collect pure starting material for analysis under visual control.
Leica Laser Capture Microdissection Products
Observing the effect on transcription factor signaling and chromatin landscapes in cells infected with the human coronavirus 229EViral infection triggers an inflammatory cellular response which is characterized by altered gene expression. In this study, the influence of coronaviruses on cellular NF-κB (transcription factor)…Read article
- Lectin microarray (LMA) is a highly sensitive technology used to obtain the global glycomic profiles of endogenous glycoproteins in biological samples including formalin-fixed paraffin-embedded tissue…Read article
Nanodroplet Processing Platform for Deep and Quantitative Proteome Proﬁling of 10–100 Mammalian CellsNanoscale or single-cell technologies are critical for biomedical applications. However, current mass spectrometry (MS)-based proteomic approaches require samples comprising a minimum of thousands of…Read article
MicroRNA Expression Profiling to Identify and Validate Reference Genes for the Relative Quantification of microRNA in Rectal CancerApplication example for laser microdissection (LMD) - MicroRNAs (miRNAs) play important roles in regulating biological processes at the post-transcriptional level. Deregulation of miRNAs has been…Read article
Total RNA Isolation after Laser-capture Microdissection of Human Cervical Squamous Epithelial Cells from Fresh Frozen TissueAs most tissue specimens contain a mixture of different cell types including epithelial cells, stromal cells and immune cells, selection of the cells of interest is of utmost importance for the…Read article
- Correlative light electron microscopy (CLEM) combines the advantages of light and electron microscopy, thus making it possible to follow dynamic events in living cells at nanometre resolution. Various…Read article
- Gliomas are mostly incurable secondary to their diffuse infiltrative nature. Thus, specific therapeutic targeting of invasive glioma cells is an attractive concept. As cells exit the tumor mass and…Read article
Glucose-Regulated Protein 78 (Grp78) Confers Chemoresistance to Tumor Endothelial Cells under Acidic StressThis study was designed to investigate the activation of the unfolded protein response (UPR) in tumor associated endothelial cells (TECs) and its association with chemoresistance during acidic pH…Read article
Sorbin and SH3 Domain‐Containing Protein 2 Is Released from Infarcted Heart in the Very Early Phase: Proteomic Analysis of Cardiac Tissues From PatientsMany animal experiments have been performed to date to elucidate the mechanism of cardiac ischemic injury, and these studies have enhanced our understanding of cardiac remodeling following hypoxic…Read article
Production of RNA for Transcriptomic Analysis from Mouse Spinal Cord Motor Neuron Cell Bodies by Laser Capture MicrodissectionHigh-quality total RNA has been prepared from cell bodies of mouse spinal cord motor neurons by laser capture microdissection after staining spinal cord sections with Azure B in 70% ethanol.…Read article
From Dynamic Live Cell Imaging to 3D Ultrastructure: Novel Integrated Methods for High Pressure Freezing and Correlative Light-Electron MicroscopyTo correlate dynamic events in adherent cells with both ultrastructural and 3D information, we developed a method for cultured cells that combines confocal time-lapse images of GFP-tagged proteins…Read article
K-ATP Channels in Dopamine Substantia Nigra Neurons Control Bursting and Novelty-induced ExplorationPhasic activation of the dopamine (DA) midbrain system in response to unexpected reward or novelty is critical for adaptive behavioral strategies. This activation of DA midbrain neurons occurs via a…Read article
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