Photomanipulation

The term photomanipulation encompasses a range of techniques that utilize the properties of fluorescent molecules to initiate events and observe how dynamic complexes behave over time in living cells.

Whether bleaching, activating, converting, ablating or combining techniques, researchers need to have a system fully capable of performing and capturing events in high resolution.

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Leica Photomanipulation Products

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THUNDER Imager 3D Cell Culture

THUNDER Imager Live Cell & 3D Cell Culture & 3D Assay

THUNDER Imagers provide you with a solution for advanced 3D cell culture assays, whether you want to study stem cells, spheroids, or organoids.

Fluorescence Recovery After Photobleaching (FRAP)

Fluorescence Recovery After Photobleaching (FRAP) experiments require fast yet gentle and accurate bleaching of specific regions of interest (ROIs).

FRAP experiments typically are used in biological studies of cellular kinetics, such as membrane diffusion and protein binding.

Leica offers two solutions for FRAP experiments with the DMi8 inverted microscope

  • The WF FRAP module is a budget-friendly entry level FRAP all-in-one device.
  • For more advanced applications, use the Infinity Scanner module to perform multi-ROI high-speed photobleaching experiments.

Activating

Utilize the latest advances in photoswitchable fluorescent proteins.

By selectively activating regions of your cells, you can precisely follow how specific molecules behave in cells over time.

The Infinity Scanner gives you full flexibility to choose the wavelength, size, and shape of your activation regions.

Full integration into LAS X microscope software

With full integration into LAS X software, you can design and perform photoactivation experiments with the ease of a fully integrated system.

Cutting/Ablation

Perform laser ablation experiments by adding the Pulsed Laser Unit to the Infinity Scanner.

Ablation experiments require high power, precise cuts to specific structures, such as microtubules, or regions of cells in order to gain insights into structural and developmental processes.

Optogenetics

Using light to stimulate  conformational changes to proteins, optogenetic techniques offer researchers the ability to control specific changes in living cells and tissues. The versatile Infinity Scanner offers user the ability to perform optogenetics experiments, and to combine with additional photomanipulation techniques into one experiment. 

Photomanipulation with DMi8 S: Flexible live cell imaging

The Leica DMi8 S microscopy system lets you easily combine applications by adding 1 or more advanced fluorescence imaging modules, such as the Infinity Scanner and Infinity TIRF. Get unprecedented flexibility allowing you to expose your sample to multiple techniques simultaneously, giving you deeper insights into your cells.

The Infinity Scanner enables a host of photomanipulation techniques, like FRAP, FLIP, photo activation as well as photo switching, optogenetics and ablation. Advance your research by combining several photo manipulation modes in one experiment, e.g. apply photo damage to the nucleus to induce DNA damage. Then use photo switching to identify and track when repair proteins are released from the DNA. Setting up and running experiments is straightforward, allowing you to see the hidden to advance your discoveries.

The Leica DMi8 S offers near infinite possibilities to adapt and expand with your research and enable your discoveries today and tomorrow.

News

10. September 2021 Role of Mucins and Glycosylation in Dry Eye Disease

This article shows how fast, high-contrast, and sharp imaging of stratified human corneal epithelial cells with THUNDER imaging technology for dry eye…

Leica Science Lab
Images of C2C12 cells which were stained with lamin B (magenta), Hoechst (blue), and yH2AX (yellow). A) raw widefield fluorescence image and B) THUNDER image after LVCC. The yellow foci indicate damaged DNA.

02. September 2021 Skeletal Muscle Adaptation and Fibrotic Diseases

The mechanisms of how skeletal muscle adapts to fibrotic pathologies can be investigated more efficiently with fast, high-contrast imaging of C2C12…

Leica Science Lab

30. August 2021 Spectroscopic Evaluation of Red Blood Cells

Hemoglobinopathies are a major healthcare problem. This study presents a possible diagnostic tool for thalassemia which is based on confocal…

Leica Science Lab

17. August 2021 Cortical Contributions to Complex Learning

This article describes the use of sharp, high contrast imaging to facilitate assessment of viral-construct placement in rat cortical brain tissue.…

Leica Science Lab

16. August 2021 Visualization of Submitochondrial Protein Distributions

By allowing visualization of submitochondrial protein distributions, fluorescence nanoscopy offers significant advantages for understanding cell death…

Leica Science Lab

27. July 2021 DNA Replication in Cancer Cells

DNA synthesis can be impeded by collisions between the DNA replication machinery and co-transcriptional R-loops leading to a major source of genomic…

Leica Science Lab

09. July 2021 Optimizing THUNDER Platform for High-Content Slide Scanning

With rising demand for full-tissue imaging and the need for FL signal quantitation in diverse biological specimens, the limits on HC imaging…

Leica Science Lab
C. elegans Gonades - THUNDER Imager  Adult hermaphrodit, Staining: blue - DAPI (Nucleus), green - SP56 (sperms), red - RME-2 (oocyte), mangenta - PGL-1 (RNA + protein granules) Image courtesy of Prof. Dr. Christian Eckmann, Martin Luther University, Halle, Germany

29. June 2021 Physiology Image Gallery

Physiology is about the processes and functions within a living organism. Research in physiology focuses on the activities and functions of an…

Leica Science Lab
THUNDER image of a section of mouse lung where the mouse was inoculated with the Puerto Rico 8 strain of influenza virus. The lung tissue was immunofluorescently stained with Keratin-5 (green) and PDL-1 (red). Image courtesy of Andrew Beppu, Stripp Lab, Cedars Sinai Medical Center, Los Angeles, USA.

29. June 2021 Monitoring Immunosuppressive Mechanisms from Infection

This application note discusses the importance of fast, sensitive, and haze-free imaging for the monitoring of immunosuppression in mouse lung…

Leica Science Lab
Virally labeled neurons (red) and astrocytes (green) in a cortical spheroid derived from human induced pluripotent stem cells. THUNDER Model Organism Imagerwith a 2x 0.15 NA objective at 3.4x zoomwas used to produce this 425 μm Z-stack (26 positions), which is presented here as an Extended Depth of Field(EDoF)projection.

25. June 2021 Neuroscience Image Gallery

Neuroscience commonly uses microscopy to study the nervous system’s function and understand neurodegenerative diseases.

Leica Science Lab

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