Photomanipulation

The term photomanipulation encompasses a range of techniques that utilize the properties of fluorescent molecules to initiate events and observe how dynamic complexes behave over time in living cells.

Whether bleaching, activating, converting, ablating or combining techniques, researchers need to have a system fully capable of performing and capturing events in high resolution.

Simply get in touch!

Want to know more on our solutions for Photomanipulation?

Leica Photomanipulation Products

Filter by Area of Application
THUNDER Imager 3D Cell Culture

THUNDER Imager Live Cell & 3D Cell Culture & 3D Assay

THUNDER Imagers provide you with a solution for advanced 3D cell culture assays, whether you want to study stem cells, spheroids, or organoids.

Fluorescence Recovery After Photobleaching (FRAP)

Fluorescence Recovery After Photobleaching (FRAP) experiments require fast yet gentle and accurate bleaching of specific regions of interest (ROIs).

FRAP experiments typically are used in biological studies of cellular kinetics, such as membrane diffusion and protein binding.

Leica offers two solutions for FRAP experiments with the DMi8 inverted microscope

  • The WF FRAP module is a budget-friendly entry level FRAP all-in-one device.
  • For more advanced applications, use the Infinity Scanner module to perform multi-ROI high-speed photobleaching experiments.

Activating

Utilize the latest advances in photoswitchable fluorescent proteins.

By selectively activating regions of your cells, you can precisely follow how specific molecules behave in cells over time.

The Infinity Scanner gives you full flexibility to choose the wavelength, size, and shape of your activation regions.

Full integration into LAS X microscope software

With full integration into LAS X software, you can design and perform photoactivation experiments with the ease of a fully integrated system.

Cutting/Ablation

Perform laser ablation experiments by adding the Pulsed Laser Unit to the Infinity Scanner.

Ablation experiments require high power, precise cuts to specific structures, such as microtubules, or regions of cells in order to gain insights into structural and developmental processes.

Optogenetics

Using light to stimulate  conformational changes to proteins, optogenetic techniques offer researchers the ability to control specific changes in living cells and tissues. The versatile Infinity Scanner offers user the ability to perform optogenetics experiments, and to combine with additional photomanipulation techniques into one experiment. 

Photomanipulation with DMi8 S: Flexible live cell imaging

The Leica DMi8 S microscopy system lets you easily combine applications by adding 1 or more advanced fluorescence imaging modules, such as the Infinity Scanner and Infinity TIRF. Get unprecedented flexibility allowing you to expose your sample to multiple techniques simultaneously, giving you deeper insights into your cells.

The Infinity Scanner enables a host of photomanipulation techniques, like FRAP, FLIP, photo activation as well as photo switching, optogenetics and ablation. Advance your research by combining several photo manipulation modes in one experiment, e.g. apply photo damage to the nucleus to induce DNA damage. Then use photo switching to identify and track when repair proteins are released from the DNA. Setting up and running experiments is straightforward, allowing you to see the hidden to advance your discoveries.

The Leica DMi8 S offers near infinite possibilities to adapt and expand with your research and enable your discoveries today and tomorrow.

News

12. May 2021 Cytosolic Calcium Ions in Melanoma Cancer Cells

In this article, a ratiometric fluorometric method for cytosolic calcium ion (Ca2+) measurement in cultured melanoma cells using Fura 2-AM cell…

Leica Science Lab
Virally labeled neurons (red) and astrocytes (green) in a cortical spheroid derived from human induced pluripotent stem cells. THUNDER Model Organism Imager with a 2x 0.15 NA objective at 3.4x zoom was used to produce this 425 µm Z-stack (26 positions), which is presented here as an Extended Depth of Field (EDoF) projection.  Images courtesy of Dr. Fikri Birey  from the Dr. Sergiu Pasca laboratory at Stanford University, 3165 Porter Dr., Palo Alto, CA

05. May 2021 Download The Guide to Live Cell Imaging

In life science research, live cell imaging is an indispensable tool to visualize cells in a state as in vivo as possible. This E-book reviews a wide…

Leica Science Lab

09. March 2021 Studying Cell Division

Cell division is a biological process during which all cellular components must be distributed among the daughter cells. The division process requires…

Leica Science Lab
The THUNDER Imager 3D Cell Culture visualizes an influenza (red) infected primary porcine lung epithelial grown in a multilayer of about 60 µm. The top layer shows cilia (green), which are responsible for mucus transport. Nuclei are stained in blue. Raw image vs. Large Volume Computational Clearing. Courtesy of Dr. Stefan Finke, Friedrich-Loeffler-Institute, Riems (Germany).

04. March 2021 Viral Infections – Studying Influenza-host Interactions in 3D Specimens

Stefan Finke studies virus-host interactions. It turns out that the ways the virus and host interact are different when comparing results from…

Leica Science Lab
Images of the scaffold composed of fluorescent fibers: Left: raw widefield image. Right: THUNDER image with LVVC. Both images are maximal projections of a z stack of 55 images (total height of 130 µm). Images courtesy of Mollie Smoak, Department of Bioengineering, Rice University, Houston, TX, USA

17. February 2021 Finding new Scaffolds for Tissue Engineering

Tissue engineers use biomaterials for a variety of applications from drug delivery to supporting the regeneration of damaged or lost tissues to…

Leica Science Lab

16. February 2021 The Power of Pairing Adaptive Deconvolution with Computational Clearing

Deconvolution is a computational method used to restore the image of the object that is corrupted by the point spread function (PSF) along with…

Leica Science Lab

15. February 2021 Improvement of Imaging Techniques to Understand Organelle Membrane Cell Dynamics

Understanding cell functions in normal and tumorous tissue is a key factor in advancing research of potential treatment strategies and understanding…

Leica Science Lab

28. January 2021 Plant Cell Development and Morphogenesis

The article discusses how tubulin molecules, which make up microtubules, in plant cells can be studied with total internal reflection fluorescence…

Leica Science Lab

27. January 2021 Studying Autoimmune Disease

This article discusses how autoimmune diseases, like systemic lupus erythematosus (SLE), can be studied more efficiently using thick, 3D kidney tissue…

Leica Science Lab
Mouse lymphnode acquired with a THUNDER Imager 3D Cell Culture. Image courtesy of Dr. Selina Keppler, Munich, Germany.

20. January 2021 Image Gallery: THUNDER Imager

To help you answer important scientific questions, THUNDER Imagers eliminate the out-of-focus blur that clouds the view of thick samples when using…

Leica Science Lab

Interested to know more?

Talk to our experts. We are happy to answer all your questions and concerns.

Contact Us

Do you prefer personal consulting?

  • Leica Microsystems Inc.
    1700 Leider Lane
    Buffalo Grove, IL 60089 United States
    Office Phone : +1 800 248 0123
    Service Phone : 1 800 248 0223
    Fax : +1 847-236-3009

You will find a more detailed list of local contacts here.