Super-Resolution Microscopy
Sub-diffraction colocalization analysis reveals interactions unprecedented detail. Enabling novel discoveries to be made in the fields of virology, immunology, neuroscience and cancer, super-resolution is on its way to becoming the new gold standard in light microscopy.
Explore life’s true nature in unprecedented detail.
Contact a local specialist for advice on the right super-resolution or nanoscopy system for your needs.
Super-resolution and nanoscopy products
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STELLARIS
With the STELLARIS confocal platform, we have re-imagined confocal microscopy to get you closer to the truth.
STELLARIS STED & STELLARIS 8 STED
STELLARIS brings the full power and potential of STED in a fully integrated system.
About Super-Resolution
It is about 20 years since super-resolution microscopy and nanoscopy arrived on the light microscopy scene, but it already plays an important role, particularly in life sciences – without superseding conventional confocal microscopy. The term super-resolution refers to methods that surpass the so-called diffraction limit. Applications are wide ranging – from dynamic vesicle movements to fluorescence images of sub-cellular structures, allowing researchers to see details in unprecedented detail. LIGHTNING Image Information Extraction is a super-resolution method that exploits the sub-diffraction lateral resolution capabilities of confocal microscopy. With LIGHTNING on a SP8 confocal microscope you can image multiple fluorophores simultaneously – without the need for sequential scanning. You can capture cellular details and observe dynamics with resolution down to 120 nm. As a truly infinitely super-resolving technology, STED nanoscopy offers resolution down to 30 nanometers. STED provides instant super-resolved imaging with multiple channels and approaching isotropic super-resolution in three dimensions. Underlining the impact of super-resolution microscopy, the 2014 Nobel Prize for Chemistry was awarded jointly to Eric Betzig, Stefan W. Hell and William E. Moerner "for the development of super-resolved fluorescence microscopy".
Related Articles
Sharp Live Images from the Mouse Brain
To explore the most intricate structures of the brain in order to decipher how it functions – Stefan Hell’s team of researchers at the Max Planck Institute for Biophysical Chemistry in Göttingen has…Read article
Dual Channel STED Nanoscopy of Lytic Granules on Actin Filaments in Natural Killer Cells
Natural killer (NK) cells are innate immune effectors that eliminate diseased and tumorigenic targets through the directed secretion of specialized secretory lysosomes, termed lytic granules. This…Read article
Video Tutorial: How to Optimize Sample Preparation for GSD Microscopy
This video tutorial presents a reliable way to produce samples for super-resolution GSD imaging with a special focus on the mounting step. Stable and flat mounting of the coverslip increases the…Read article
Videos on STED Microscopy and its Inventor Stefan Hell
Here we present an collection of videos telling the story of the invention of nanometer-scale microscopy by Stefan Hell and explaining the basic principle of STED microscopy.Read article
Subcellular Localization of AKT and Tubulin using Super-Resolution Microscopy
Stimulated Emission Depletion microscopy, or STED nanoscopy, is a technique that uses the non-linear de-excitation of fluorescent dyes to overcome the resolution limit imposed by diffraction…Read article
Two-color STED Microscopy of Living Synapses using a Single Laser-beam Pair
The advent of superresolution microscopy has opened up new research opportunities into dynamic processes at the nanoscale inside living biological specimens. This is particularly true for synapses,…Read article
Applications, Labeling Strategies and Fluorophores for Super-Resolution
The free online webinar on super-resolution presented by Leica Microsystems in association with Microscopy & Analysis took place on Tuesday, 15 November 2011. Register and view the webinar on demand.Read article
Super-Resolution GSDIM Microscopy
The nanoscopic technique GSDIM (ground state depletion microscopy followed by individual molecule return) provides a detailed image of the spatial arrangement of proteins and other biomolecules within…Read article
Natural Killer Cell Lytic Granule Secretion Occurs through a Pervasive Actin Network at the Immune Synapse
Accumulation of filamentous actin (F-actin) at the immunological synapse (IS) is a prerequisite for the cytotoxic function of natural killer (NK) cells. Subsequent to reorganization of the actin…Read article
STED Nanoscopy of Actin Dynamics in Synapses deep inside Living Brain Slices
It is difficult to investigate the mechanisms that mediate long-term changes in synapse function because synapses are small and deeply embedded inside brain tissue. Although recent fluorescence…Read article
Nanoscopy in a Living Multicellular Organism Expressing GFP
We report superresolution fluorescence microscopy in an intact living organism, namely Caenorhabditis elegans nematodes expressing green fluorescent protein (GFP)-fusion proteins. We also…Read article
The Missing Link to the Nanocosm of Life
Fully understanding the functionality and complexity of the human central nervous system remains one of the major open questions in modern science. Stimulated emission depletion microscopy (STED) can…Read article
Restless Receptors
Synapses are the switch-points in our brain for information transmission, learning and memory. News studies and developments of imaging techniques have provided new insights into the dynamics of…Read article
The Fate of Synaptic Vesicle Components upon Fusion
Neurotransmitter release relies on the fusion of synaptic vesicles with the plasma membrane of synaptic boutons, which is followed by the recycling of vesicle components and formation of new vesicles.…Read article
A Guide to Super-Resolution Fluorescence Microscopy
For centuries, cell biology has been based on light microscopy and at the same time been limited by its optical resolution. However, several new technologies have been developed recently that bypass…Read article
Confocal Nanoscopy Goes Multicolor
Scientists strive to understand the architecture of life. They want to learn how biological structures are arranged in respect to one another. Multicolor superresolution imaging allows fundamental…Read article
In Vivo Labeling Method Using a Genetic Construct for Nanoscale Resolution Microscopy
We demonstrate beam scanning-stimulated emission depletion microscopy with in vivo labeled cells. A red emitting fluorescent dye is introduced into membrane protein fused to a multifunctional reporter…Read article
Interview with Stefan Hell – For me, Pioneering is …
Our interview partner on the topic of Pioneering is Prf. Stefan Hell, a scientific member of the Max Planck Society and director at the Max Planck Institute for Biophysical Chemistry in Göttingen,…Read article
Useful Links
Super-Resolution & Nanoscopy Publications
Communities and Web Sources
www.researchgate.net/Social network for scientists
www.lsoft.com/scripts/wl.exe?SL1=CONFOCALMICROSCOPY&H=LISTS.UMN.EDUConfocal Microscopy Mailing List, University of Minnesota
www.ibiology.org/Teaching tools, video lectures on biology and microscopy
bitesizebio.comOnline magazine and community for molecular and cell biology researchers
Search Engines and Data Bases
www.cellimagelibrary.orgPublic resource database of images, videos, and animations of cells
harvester.fzk.de/harvesterBioinformatic meta search engine for genes and proteins
www.gopubmed.comSearch interface for pubmed
en.wikipedia.org/wiki/List_of_academic_databases_and_search_enginesList of academic databases and search engines
scholar.google.comBeta of Google's search engine for scientific article abstracts
Journals
www.optnano.com/Optical Nanoscopy
www.doaj.org/Directory of open access journals
emboj.embopress.org/The EMBO Journal
www.lifescied.orgCBE-Life Sciences Education – an ASCB online journal
www.sciencemag.org/Science
www.nature.com/Nature
www.cell.com/Biweekly publication of exceptional research articles
jcs.biologists.org/Journal of Cell Science
dev.biologists.org/Development
jeb.biologists.org/The Journal of Experimental Biology
dmm.biologists.org/DMM Disease Models & Mechanisms
www.biotechniques.com/International Journal of Life Science Methods
www.opticsinfobase.org/Collection of Journals and Proceedings in Optics and Photonics
spie.org/x576.xmlSPIE - peer-reviewed journals on applied research in optics and photonics
onlinelibrary.wiley.com/journal/10.1002/(ISSN)1864-0648Journal of Biophotonics
www.plosone.org/International, peer-reviewed, open-access, online publication
rspb.royalsocietypublishing.org/Proceedings B - the Royal Society's biological research journal
www.microscopy-analysis.com/International Journal for microscopists
Organizations
www.microscopy.org/Microscopy Society of America
www.eurmicsoc.org/European Microscopy Society
www.rms.org.uk/Royal Microscopical Society
www.ascb.org/ASCB American Society of Cell Biology
www.biologists.com/cob_activities.htmlthe company of biologists
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