

Super-Resolution Microscopy
Sub-diffraction colocalization analysis reveals interactions unprecedented detail. Enabling novel discoveries to be made in the fields of virology, immunology, neuroscience and cancer, super-resolution is on its way to becoming the new gold standard in light microscopy.
Explore life’s true nature in unprecedented detail.
Contact a local specialist for advice on the right super-resolution or nanoscopy system for your needs.
Super-resolution and nanoscopy products
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STELLARIS
With the STELLARIS confocal platform, we have re-imagined confocal microscopy to get you closer to the truth.

STELLARIS STED & STELLARIS 8 STED
STELLARIS brings the full power and potential of STED in a fully integrated system.
About Super-Resolution
It is about 20 years since super-resolution microscopy and nanoscopy arrived on the light microscopy scene, but it already plays an important role, particularly in life sciences – without superseding conventional confocal microscopy. The term super-resolution refers to methods that surpass the so-called diffraction limit. Applications are wide ranging – from dynamic vesicle movements to fluorescence images of sub-cellular structures, allowing researchers to see details in unprecedented detail. LIGHTNING Image Information Extraction is a super-resolution method that exploits the sub-diffraction lateral resolution capabilities of confocal microscopy. With LIGHTNING on a SP8 confocal microscope you can image multiple fluorophores simultaneously – without the need for sequential scanning. You can capture cellular details and observe dynamics with resolution down to 120 nm. As a truly infinitely super-resolving technology, STED nanoscopy offers resolution down to 30 nanometers. STED provides instant super-resolved imaging with multiple channels and approaching isotropic super-resolution in three dimensions. Underlining the impact of super-resolution microscopy, the 2014 Nobel Prize for Chemistry was awarded jointly to Eric Betzig, Stefan W. Hell and William E. Moerner "for the development of super-resolved fluorescence microscopy".
Related Articles
Sharp Live Images from the Mouse Brain
To explore the most intricate structures of the brain in order to decipher how it functions – Stefan Hell’s team of researchers at the Max Planck Institute for Biophysical Chemistry in Göttingen has…Read articleDual Channel STED Nanoscopy of Lytic Granules on Actin Filaments in Natural Killer Cells
Natural killer (NK) cells are innate immune effectors that eliminate diseased and tumorigenic targets through the directed secretion of specialized secretory lysosomes, termed lytic granules. This…Read articleVideo Tutorial: How to Optimize Sample Preparation for GSD Microscopy
This video tutorial presents a reliable way to produce samples for super-resolution GSD imaging with a special focus on the mounting step. Stable and flat mounting of the coverslip increases the…Read articleVideos on STED Microscopy and its Inventor Stefan Hell
Here we present an collection of videos telling the story of the invention of nanometer-scale microscopy by Stefan Hell and explaining the basic principle of STED microscopy.Read articleSubcellular Localization of AKT and Tubulin using Super-Resolution Microscopy
Stimulated Emission Depletion microscopy, or STED nanoscopy, is a technique that uses the non-linear de-excitation of fluorescent dyes to overcome the resolution limit imposed by diffraction…Read articleTwo-color STED Microscopy of Living Synapses using a Single Laser-beam Pair
The advent of superresolution microscopy has opened up new research opportunities into dynamic processes at the nanoscale inside living biological specimens. This is particularly true for synapses,…Read articleApplications, Labeling Strategies and Fluorophores for Super-Resolution
The free online webinar on super-resolution presented by Leica Microsystems in association with Microscopy & Analysis took place on Tuesday, 15 November 2011. Register and view the webinar on demand.Read articleSuper-Resolution GSDIM Microscopy
The nanoscopic technique