Sub-diffraction colocalization analysis reveals interactions unprecedented detail. Enabling novel discoveries to be made in the fields of virology, immunology, neuroscience and cancer, super-resolution is on its way to becoming the new gold standard in light microscopy.
Explore life’s true nature in unprecedented detail.
Super-resolution and nanoscopy products
It is about 20 years since super-resolution microscopy and nanoscopy arrived on the light microscopy scene, but it already plays an important role, particularly in life sciences – without superseding conventional confocal microscopy. The term super-resolution refers to methods that surpass the so-called diffraction limit. Applications are wide ranging – from dynamic vesicle movements to fluorescence images of sub-cellular structures, allowing researchers to see details in unprecedented detail. LIGHTNING Image Information Extraction is a super-resolution method that exploits the sub-diffraction lateral resolution capabilities of confocal microscopy. With LIGHTNING on a SP8 confocal microscope you can image multiple fluorophores simultaneously – without the need for sequential scanning. You can capture cellular details and observe dynamics with resolution down to 120 nm. As a truly infinitely super-resolving technology, STED nanoscopy offers resolution down to 30 nanometers. STED provides instant super-resolved imaging with multiple channels and approaching isotropic super-resolution in three dimensions. Underlining the impact of super-resolution microscopy, the 2014 Nobel Prize for Chemistry was awarded jointly to Eric Betzig, Stefan W. Hell and William E. Moerner "for the development of super-resolved fluorescence microscopy".
- When it comes to STED Nanoscopy, keeping high signal-to-noise is key to achieve the best possible resolution in fixed and living cells. This can be challenging in the case of experiments in 3D and/or…Read article
- Confocal Laser Scanning Microscopy (CLSM) is the standard for true 3D resolved fluorescence imaging. Fast optical sectioning using flexible scanning strategies in combination with simultaneous…Read article
- Malaria is a life-threatening disease transmitted through the bites of mosquitoes infected with protozoan parasites. The most common and dangerous type of malaria is caused by the parasite Plasmodium…Read article
- Alison Dun is the postdoctoral facility manager for the Edinburgh Super-Resolution Imaging Consortium (ESRIC), Heriot-Watt University, Edinburgh, UK. She has used a large range of microscope…Read article
- A list of the published scientific articles which include work done in the ALMS/MSI Facilities.Read article
- The 7th Super-Resolution User Club Meeting was held in collaboration with Prof Pavel Hozák , at the Institute of Molecular Genetics of the ASCR in Prague. Keeping the event close to science is one of…Read article
- Do you know that most protists and bacteria lack in one feature that each of our body cell has? Our cells are touch and communicate with one another. They send and receive a variety of signals that…Read article
- The 6th European Super-Resolution User Club Meeting was held in collaboration with Dr. Timo Zimmermann, CRG, and Dr. Pablo Loza-Alvarez, ICFO, Barcelona. According to the founding principle of the…Read article
- A new type of fluorescent bead is presented by GATTAquant. These beads, called GATTA-Beads, are characterized by a small diameter (23 nm), high intensity and size uniformity. In combination with…Read article
Quantifying the Resolution of a Leica SR GSD 3D Localization Microscopy System with 2D and 3D NanorulersDNA origami based nanorulers produced by GATTAquant are common standards to test the achievable spatial resolution of super-resolution microscopes. Recently the nanorulers were used to test the…Read article
- Since the invention of the microscope, there has been continual discussion about the possibility of showing more detailed features of specimens as compared to just magnifying them. In this article we…Read article
- Bram van den Broek is a postdoctoral fellow at the Netherlands cancer institute in Amsterdam where he supports the advanced microscopy techniques in the laboratory of Kees Jalink. Working with Leica…Read article
- Here we show staining of HDAC1 in cancer tissue and epidermoid carcinoma cells. These results clearly show that the use of appropriate validated antibodies and STED microscopy are important tools to…Read article
- The 5th Super-Resolution User Club Meeting was held in collaboration with Professor Kees Jalink and The Netherlands Cancer Institute (NKI) in Amsterdam. Having the meeting at a location where…Read article
- Only a few days to go before the start of Focus on Microscopy 2015 in Göttingen, Germany. This year’s FOM is being organized by Dr. Gertrude Bunt and Prof. Dr. Fred S. Wouters from the University…Read article
- For Professor Stefan Hell, who won the Nobel Prize for Chemistry in 2014 for the development of super-resolved fluorescence microscopy and the development of STED microscopy, the Focus on Microscopy…Read article
- Super-resolution microscopy techniques have revolutionized biology for the last ten years. With their help cellular components can now be visualized at the size of a protein. Nevertheless, imaging…Read article