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U2OS cell, human osteosarcoma Mitochondria - low light exposure

THUNDER - low light exposure

U2OS cell, human osteosarcoma Mitochondria labelled with MitoView green; analysis result from THUNDER and Aivia.

THUNDER and Aivia

Save time and effort with AI-assisted fluorescence image analysis.

  • Clarity and accuracy for segmentation
  • More time, more data, more images
  • AI and application expertise, all in one place

Discover the synergy of THUNDER and Aivia.

U2OS cell, human osteosarcoma Mitochondria labelled with MitoView green; analysis result from THUNDER and Aivia.

Reach discovery goals with greater accuracy

Crisp THUNDERed images remove the blur and haze you get through conventional widefield imagers, allowing clear marking of signal and background.

The powerful synergy of THUNDER and Aivia analyze fluorescence images with greater accuracy, even when using low light excitation.

Time lapse showing mitochondria. Analysis of THUNDER images. Compared to the analysis of raw data, a larger number of objects can be seen, indicating a better (more accurate) segmentation. Consequently, the tracking is more precise and any information extracted (displacement, speed, form factor, size, shape) is less prone to error.

Increase scientific capabilities

The unique workflow is especially powerful when using thick samples, working with living specimens when low light dosages for excitation are crucial, performing time-lapse experiments, or capturing large image stacks, making it easier for you to more precisely analyze objects of interest.

Your dedicated Leica expert has the know-how to get the detailed image acquisition and analysis results you want and need, giving you the freedom to focus on your discoveries.

Analysis of raw data.

This is the input dataset for the analyzed timeseries.

The signal is not very strong and the background and noise levels are quite unfavorable.

More analysis possibilities with THUNDER and Aivia

Learn how the synergy of THUNDER and Aivia help accelerate your research. Request a call back from your dedicated Leica account manager.

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The knowledge portal of Leica Microsystems offers scientific research and teaching material on the subjects of microscopy. The content is designed to support beginners, experienced practitioners and scientists alike in their everyday work and experiments.

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