Empowering scientists to reach their discovery goals faster.


U2OS cell, human osteosarcoma Mitochondria - low light exposure

THUNDER - low light exposure

U2OS cell, human osteosarcoma Mitochondria labelled with MitoView green; analysis result from THUNDER and Aivia.

THUNDER and Aivia

Save time and effort with AI-assisted fluorescence image analysis.

  • Clarity and accuracy for segmentation
  • More time, more data, more images
  • AI and application expertise, all in one place

Discover the synergy of THUNDER and Aivia.

U2OS cell, human osteosarcoma Mitochondria labelled with MitoView green; analysis result from THUNDER and Aivia.

Reach discovery goals with greater accuracy

Crisp THUNDERed images remove the blur and haze you get through conventional widefield imagers, allowing clear marking of signal and background.

The powerful synergy of THUNDER and Aivia analyze fluorescence images with greater accuracy, even when using low light excitation.

Time lapse showing mitochondria. Analysis of THUNDER images. Compared to the analysis of raw data, a larger number of objects can be seen, indicating a better (more accurate) segmentation. Consequently, the tracking is more precise and any information extracted (displacement, speed, form factor, size, shape) is less prone to error.

Increase scientific capabilities

The unique workflow is especially powerful when using thick samples, working with living specimens when low light dosages for excitation are crucial, performing time-lapse experiments, or capturing large image stacks, making it easier for you to more precisely analyze objects of interest.

Your dedicated Leica expert has the know-how to get the detailed image acquisition and analysis results you want and need, giving you the freedom to focus on your discoveries.

Analysis of raw data.

This is the input dataset for the analyzed timeseries.

The signal is not very strong and the background and noise levels are quite unfavorable.

More analysis possibilities with THUNDER and Aivia

Learn how the synergy of THUNDER and Aivia help accelerate your research. Request a call back from your dedicated Leica account manager.

Discover more today

By clicking on the SUBMIT button, I confirm that I have reviewed and agree with Leica Microsystems GmbH Terms of Use and their Privacy Policy. I also understand my privacy choices as they pertain to my personal data, as detailed in the aforementioned Privacy Policy under ‘’Your Privacy Choices’’.

Read our latest articles about THUNDER and Aivia

The knowledge portal of Leica Microsystems offers scientific research and teaching material on the subjects of microscopy. The content is designed to support beginners, experienced practitioners and scientists alike in their everyday work and experiments.

More Articles

3D reconstruction of an isolated human islet

Create New Options for Live Cell Imaging

The use of state-of-the-art AI systems is pushing image analysis into a new generation. Challenges like the conflict between imaging power and sample integrity are being overcome with THUNDER’s…
Read article
Raw widefield and THUNDER image of a mouse dorsal root ganglion with tdTomato (red) expressed in the sensory neurons.

Fast, High-contrast 3D Imaging of Sensory Neurons

This article discusses how fast, high-contrast 3D imaging of dorsal root ganglion (DRG) tissue with a THUNDER Imager Tissue using large volume computational clearing (LVCC) allows sensory neurons to…
Read article
Raw widefield and THUNDER image of a mouse aorta

Molecular Mechanisms of Vascular Disease

This article discusses how the lamina, vascular cells, and nuclei of mouse aorta are more clearly resolved with a THUNDER Imager Tissue using Computational Clearing (CC) compared to conventional…
Read article
Mouse brain showing D2-dopamine receptors

Expression of D2-Dopamine Receptors in Neurons

This article discusses how D2-dopamine receptors (D2R) in the substantia-nigra of mouse brain can be more clearly revealed with a THUNDER Imager 3D Assay using Instant Computational Clearing (ICC)…
Read article
Images of mouse lung: (left) raw widefield and (right) after the THUNDER technology instant computational clearing (ICC). Courtesy of Dr. Ross Metzger, CA, USA.

Investigating Pulmonary Vascular Diseases

This article describes how the cellular and molecular programs governing the formation and maintenance of lung blood vessels, as well as what goes wrong with them when pulmonary vascular diseases…
Read article
THUNDER images of chick embryo brain with labelled neuronal progenitors: left) Raw widefield data and right) after computational clearing. Courtesy T. Sanders, University of Chicago, USA.

Deciphering Signaling Pathways in Developmental Biology

This article shows how sharp, rapid imaging of neuronal progenitors in chick embryos with a THUNDER Imager Model Organism and small volume computational clearing (SVCC) could help with the study of…
Read article
Mouse retina_widefield and LVCC

Visualizing retinal interactions to study eye diseases

This article shows how interactions between endothelial cells, blood vessels, microglia, and astrocytes in mouse retina can be studied efficiently with a THUNDER Imager 3D Cell Culture and Large…
Read article
Whole mouse embryo widefield data and LVCC

Cranial Nerve Development

This article demonstrates how fast, high-contrast imaging of mouse embryos with a THUNDER Imager 3D Cell Culture and Large Volume Computational Clearing (LVCC) enable the investigation of axonal…
Read article
Macroscopic extended depth of field images of C. elegans

Autophagy and Age-Related Pathologies

High-contrast imaging of the model organism C. elegans, a nematode, with the THUNDER Imager Model Organism using Small Volume Computational Clearing (SVCC) helps to better understand how autophagy and…
Read article
Raw widefield (left) and THUNDER (right) image of Ewing Sarcoma cells (SK-ES-1)

Visualizing the Mitotic Spindle in Cancer Cells

This article demonstrates how this research is aided by visualizing more details of mitotic spindles in Ewing Sarcoma cells using the THUNDER Imager Tissue and Large Volume Computational Clearing…
Read article
Fluorescently stained mouse tibia, imaged with a THUNDER Imager 3D Tissue. Courtesy of Dr. Anjali Kusumbe, University of Oxford, UK.

High-resolution 3D Imaging to Investigate Tissue Ageing

Award-winning researcher Dr. Anjali Kusumbe demonstrates age-related changes in vascular microenvironments through single-cell resolution 3D imaging of young and aged organs.
Read article

Role of Mucins and Glycosylation in Dry Eye Disease

This article shows how fast, high-contrast, and sharp imaging of stratified human corneal epithelial cells with THUNDER imaging technology for dry eye disease (DED) research allows membrane ridges to…
Read article
Images of C2C12 cells

Skeletal Muscle Adaptation and Fibrotic Diseases

The mechanisms of how skeletal muscle adapts to fibrotic pathologies can be investigated more efficiently with fast, high-contrast imaging of C2C12 mouse myoblast cells which is described in this…
Read article

Cortical Contributions to Complex Learning

This article describes the use of sharp, high contrast imaging to facilitate assessment of viral-construct placement in rat cortical brain tissue. Complex learning is often investigated through use of…
Read article

Optimizing THUNDER Platform for High-Content Slide Scanning

With rising demand for full-tissue imaging and the need for FL signal quantitation in diverse biological specimens, the limits on HC imaging technology are tested, while user trainability and…
Read article
C. elegans Gonades

Physiology Image Gallery

Physiology is about the processes and functions within a living organism. Research in physiology focuses on the activities and functions of an organism’s organs, tissues, or cells, including the…
Read article