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![[Translate to chinese:] Multicolor TauSTED Xtend 775 for Cell Biology applications that require nanoscopy resolution for multiple cellular components. Cells showing vimentin fibrils (AF 594), actin network (ATTO 647N), and nuclear pore basket (CF 680R). Sample courtesy of Brigitte Bergner, Mariano Gonzales Pisfil, Steffen Dietzel, Core Facility Bioimaging, Biomedical Center, Ludwig-Maximilians-University, Munich, Germany.](/fileadmin/_processed_/5/4/csm_Triple_color_fixed_sample_TauSTED_Xtend_2_3_5d4d9caa21.jpg "[Translate to chinese:] Multicolor TauSTED Xtend 775 for Cell Biology applications that require nanoscopy resolution for multiple cellular components. Cells showing vimentin fibrils (AF 594), actin network (ATTO 647N), and nuclear pore basket (CF 680R).")
STED样品制备指南
这份指南旨在帮助用户优化受激发射损耗（STED）纳米成像的样品制备，特别是在使用徕卡微系统的STED显微镜时。它提供了单色STED成像用荧光标记的概述，并对其性能进行了评级。
![[Translate to chinese:] Cell counts for each biomarker were divided by total number of cells to give a percentage of biomarker positive cells out of total cells for each biomarker.](/fileadmin/_processed_/5/3/csm_Cell_counts_for_biomarkers_with_Cell_DIVE_ee648b397a.jpg "[Translate to chinese:] Cell counts for each biomarker were divided by total number of cells to give a percentage of biomarker positive cells out of total cells for each biomarker.")
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![[Translate to chinese:] PDAC Multiplexed imaging of CST panels enables an examination of immune cell components in pancreatic ductal adenocarcinoma (IPDAC) tissue on a single slide.](/fileadmin/_processed_/a/d/csm_Pancreatic_ductal_adenocarcinoma_tissue_3d61c18a2d.jpg "[Translate to chinese:] PDAC Multiplexed imaging of CST panels enables an examination of immune cell components in pancreatic ductal adenocarcinoma (IPDAC) tissue on a single slide.")
表征肿瘤环境以揭示洞察和空间分辨率
肿瘤环境的表征可以为癌症进展和潜在治疗靶点提供更深入的见解。我们已经使用来自Cell Signaling Technology（CST）的各种IHC验证抗体，在胰腺癌的Cell DIVE研究中验证了30多种偶联抗体。
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![[Translate to chinese:] Pancreatic ductal adenocarcinoma tissue section imaged with Cell DIVE](/fileadmin/_processed_/f/8/csm_Pancreatic_ductal_adenocarcinoma_tissue_section_teaser_eae5765765.jpg "[Translate to chinese:] Pancreatic ductal adenocarcinoma tissue section imaged with Cell DIVE")
多重成像的类型、优势和应用
与传统显微镜相比，多重成像技术能观察到更多的生物标记物，是一种新兴的、令人兴奋的从人体组织样本中提取信息的方法。通过同时观察多种生物标记物，可以协同探索以前只能单独探索的生物通路，并识别和探测复杂的组织和细胞表型。目前已有许多不同的多重成像方法，每种方法都采用不同的方法来实现更高的复杂性。
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![[Translate to chinese:] Pancreatic ductal adenocarcinoma tissue section imaged with Cell DIVE showing 30 biomarkers](/fileadmin/_processed_/7/2/csm_Pancreatic_ductal_adenocarcinoma_Cell_DIVE_30_biomarkers_d1dc867094.jpg "[Translate to chinese:] Pancreatic ductal adenocarcinoma tissue section imaged with Cell DIVE showing 30 biomarkers")
多重成像术语表
人体组织中含有许多不同的生物标记物，通过对这些标记物的研究，可以揭示生物通路的工作原理，并对疾病状态进行分析。最近的技术进步使得利用超多标免疫荧光成像技术同时观察多种生物标记物成为可能。为了阐明这一新兴技术的细节，我们整理了一份重要术语表，并说明 Cell DIVE 如何帮助您将超多标免疫荧光技术应用到研究中。
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![[Translate to chinese:] Normal skin imaged with Cell DIVE, 11 biomarkers shown.](/fileadmin/_processed_/2/6/csm_Skin_imaged_with_Cell_DIVE_11_biomarkers_shown_8e5641fabb.jpg "[Translate to chinese:] Normal skin imaged with Cell DIVE, 11 biomarkers shown.")
黑色素瘤的肿瘤 MHC 表达和瘤内 IL2 反应
通过基因组学分析和Cell DIVE超多标免疫荧光成像技术，研究人员可以了解最能预测黑色素瘤转移患者对 IL2 免疫疗法反应的免疫细胞表型。
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![[Translate to chinese:] Colon adenocarcinoma with 13 biomarkers shown](/fileadmin/_processed_/4/9/csm_Colon_adenocarcinoma_with_13_biomarkers_shown_42edcfed0a.jpg "[Translate to chinese:] Colon adenocarcinoma with 13 biomarkers shown")
利用Cell DIVE 在单细胞水平上进行超复杂癌症组织分析
能够研究淋巴瘤细胞的异质性如何受到细胞对其微环境反应的影响，尤其是在突变、转录组和蛋白质水平上。蛋白质表达研究提供了有关细胞相互作用性质和蛋白质表达水平的最相关信息。超复合工作流程可用于研究同一癌症组织中的多种蛋白质。
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如何为免疫荧光显微镜制备样本
免疫荧光（IF）是一种用于可视化观察细胞内过程、状态和结构的强大工具。IF制剂可通过多种显微镜技术（如激光共聚焦、宽场荧光、全内反射成像等）来加以分析，具体取决于应用目的或研究人员的关注重点。与此同时，在很多使用至少一套简易荧光显微镜的研究工作组当中，IF早已成为不可缺少的一部分。