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通过光遗传和电刺激技术研究纳米桥接结构和动力学
纳米级超微结构信息通常是由经固定和处理样品的静态图像获得的。但是,这些静态图像只是不断变化的动态结构中的一个瞬间。因此,如何探索动态过程中的特定时间点,是纳米级超微结构研究的一个重大挑战。通过光遗传或电刺激技术,并结合毫秒级样品玻璃化技术探索纳米级超微结构,是一种解决上述问题具有前景的技术。在本应用白皮书的第一部分中,我们将从实际应用角度讨论光刺激辅助的样品玻璃化工作流程。
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Expanding the Limits of Electron Microscopy Sample Preparation
Capturing the intricate changes in fine structure or in cell dynamics with conventional cryo solutions can be challenging sometimes. Leica Microsystems has developed a new cryo platform, the Leica EM…
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Macroscale to Nanoscale Pore Analysis of Shale and Carbonate Rocks
Physical porosity in rocks, like shale and carbonate, has a large effect on the their storage capacity. The pore geometries also affect their permeability. Imaging the visible pore space provides…
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用显微镜观察结构——借助激光光谱了解结构成分
本报告介绍了使用光学显微镜和激光诱导击穿光谱(LIBS) 二合一材料分析解决方案进行同步视觉和化学检测的优势。报告还解释了二合一解决方案的基本工作原理,并将它与其它常用材料分析方法进行了对比,例如扫描电子显微镜…
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Porous Ceramics - Sample Preparation for SEM
Application Note for Leica EM RES102 - Ceramic membrane filters with pore sizes down to a few nanometres must be investigated in cross-section with regard to the structure of the pores. The smallest…
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Paper Samples - Sample Preparation for SEM
Application Note for Leica EM RES102 - A coated paper sample has been prepared with ion beam slope cutting in order to test the procedure with regard to its applicability. With the use of ion beam…
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细菌实验方案 - 对大肠杆菌样本实施临界点干燥以进行SEM分析
徕卡EM CPD300的应用文档 - 生命科学研究,对大肠杆菌样本实施临界点干燥后,进行铂/钯金属镀膜,后用SEM拍摄微观形貌,将样本放入一个滤盘中(孔径:16-40μm),置入滤盘和多孔样品架内。在含有培养基的琼脂上培养真菌和细菌,为期3天。选取部分细菌菌落。
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Ion Beam Polishing of Sample Surfaces - Sample Preparation for SEM
Application Note for Leica EM RES102 - Ion milling can be used to reduce the roughness of sample surfaces. Small angles less than 6° with respect to the sample surface are necessary. The high voltage…