How to Prepare Biological Samples for cryo-FIB Milling and cryo-EM
Tuesday 11th October: 10:00 London | 11:00 Berlin | 13:00 Dubai
Cryo-electron microscopy is becoming an important tool in biomedical research, allowing researchers to explore the cellular landscape in its native state and at high spatial resolution. A prerequisite to obtaining that goal is the preparation of electron transparent samples through cryo-FIB milling. This webinar highlights all steps in the workflow, from capturing the live sample to the perfect cryo-EM image.
In this webinar, experts from Human Technopole and Leica Microsystems will cover the different tools used to prepare cellular and biological samples, from initial vitrification and high pressure freezing, towards cryo-planing of bulk samples and confocal microscopy under cryo conditions.
A panel discussion will follow the presentations, during which our experts will take questions from the audience.
Also available on demand - register your interest above.
Key Learnings:
• How to effectively vitrify samples for on-grid lamella and lift-out procedures
• Cryo-planing methodology for bulk samples
• Cryo-CLEM acquisition routines and best practices
• Illustrations of samples prepared via FIB milling