Zebrafish, 뇌(생체 내)
Transgenic embryo, Danio rerio; GFP; Epithalamus; Optic Tectum. The images illustrate the zoom flexibility of Leica HCS LSI: The optical zoom is used first, then a combination of optical and confocal zoom is applied. The image shows a maximum projection of a 3D stack using a Leica Z6 APO A optical zoom and 5x objective.
Courtesy of K. Palma, N. Guerrero, L. Armijo, ML. Concha, Laboratory of Experimental
Ontogeny (LEO), S. Härtel, Laboratory of Scientific Image Analysis (SCIANLAB). Anatomy and Developmental Biology Program, ICBM, Faculty of Medicine, University of Chile, Santiago, Chile.
Advanced Time Lapse: 4D Single Well Experiment Zebrafish Development
Tracking the development of life over time offers exciting insights for embryogenesis – all in the same specimen. From egg to embryo, obtain exciting views of organ development with Leica HCS LSI software and see the backbone formation during the growth of a zebrafish.
Zebrafish, novocord development, Danio Rerio. Red: Rhodamine-dextran. Green: GFP, labeling of the notochord.
Courtesy of Sophie Dal-Pra, Team B&C Thisse, Imaging Centre of IGBMC, IGBMC, llkirch, France.
High content screening in 4D
Brain research: automated 4D imaging discloses the spatio-temporal dynamics of fluorescent signals throughout development.
4D series of 2 day old zebrafish embryos, Danio rerio. Ventral views of embryonic brains of the ETvmat2:gfp stable transgenic line were automatically acquired every 38 min. GFP: neurons in the brain and expression in heart and blood vessels. The embryos were arrayed in 96-well plates. The regions of interest (brains) were manually selected using the Leica Mark & Find mode and imaged at high resolution.
Courtesy of J. Gehrig, KIT – Karlsruhe Institute of Technology, Institute of Toxicology and Genetics (ITG), Karlsruhe, Germany