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Cutting of Samples

Leica Laser Microdissection Systems (LMD6000, LMD6500 and LMD7000) enable the user to easily mark regions of interest for dissection. This video presents the easy-to-use Leica LMD Software (Version 7.3) for marking single cells with the free hand tool, predefined ellipses, circles or rectangles and for collecting the marked cells by dissection with the laser of a Leica LMD7000. 
(objective: 40x; contrast: BF; specimen: rat embryo)

Cutting and Recovering of Samples

Leica Laser Microdissection Systems offer the unique and patented laser movement by optics. The laser movement by optics allows direct laser application to a sample simply by a mouse click or directly on a touch screen with a pen. This video presents the possibility to use the laser live in real time to recover a not completely dissected single cell.
(objective: 40x; contrast: BF; specimen: rat embryo)

Inspecting the Collection Device

Leica Laser Microdissection Systems are based on an upright microscope. Dissectates fall simply by gravity into a collection device. This video presents the cap inspection, which allows to visualize the dissectate in the collection device – in this case: a 0.5 ml PCR tube.
(objective: 40x and 20x; contrast: BF; specimen: rat embryo)

Creating a Fast Specimen Overview

Leica Laser Microdissection Systems offer within the core software a smart and fast specimen overview for better orientation and navigation through the specimen.  This video presents the creation of a fast specimen overview of a whole slide. The specimen overview is displayed in a separate window as well as in the Slide Holder area of the Leica LMD Software main window.
(objective: 1.25x; contrast: BF; specimen: rat embryo)

Creating a Defined Region Overview

Leica Laser Microdissection Systems offer within the core software the opportunity to create an overview of a region of interest.  This video presents the creation of such an overview and how to store the overview as an image file.
(objective: 10x; contrast: BF; specimen: rat embryo)

Laser Calibration

Leica Laser Microdissection Systems offer the unique and patented laser movement by optics. The laser can be (re-)aligned easily if needed. This video presents the comfortable and time saving procedure for a LMD laser calibration.
(objective: 10x; contrast: BF)

Laser Adjustment

Leica Laser Microdissection Systems are equipped with a high power and very flexible solid-state UV-laser. This video shows the adjustment of an LMD7000 laser to a specimen: First the laser is reset to the factory settings, afterwards the sliders for laser Power, the patented laser Aperture, Speed, Specimen Balance, Head Current and Pulse Frequency are adjusted to the sample to achieve a thin and fine cutting line suitable for laser microdissection.
(objective: 40x; contrast: BF; specimen: rat embryo)