Loading...
How Marine Microorganism Analysis can be Improved with High-pressure Freezing
In this application example we showcase the use of EM-Sample preparation with high pressure freezing, freeze substiturion and ultramicrotomy for marine biology focusing on ultrastructural analysis of…
Loading...
Advancing Cellular Ultrastructure Research
Freeze-fracture and freeze-etching are useful tools for studying flexible membrane-associated structures such as tight junctions or the enteric glycocalyx. Freeze-fracture and etching are two…
Loading...
超薄切片介绍
对样本开展研究时,为了以纳米级分辨率显示其精细结构,通常会使用到电子显微镜。电子显微镜有两种类型:扫描电子显微镜(SEM)用于对样本表面成像,以及需要使用极薄电子透明样本的透射电子显微镜(TEM)。因此,使用电子显微镜对样本内部的精细结构进行成像时,此类技术解决方案需要制作出非常薄的样本切片。被称为超显微技术的样本制备方法可以产生具有最小伪影的超薄切片(厚度20-150nm)。在切片过程中,样本的…
Loading...
Visualization of DNA Molecules
Precise low angle rotary shadowing with heavy metals (like platinum) can be used in transmission electron microscopy (TEM) to observe molecular details of objects previously absorbed on a thin, low…
Loading...
Ion Beam Polishing of Sample Surfaces - Sample Preparation for SEM
Application Note for Leica EM RES102 - Ion milling can be used to reduce the roughness of sample surfaces. Small angles less than 6° with respect to the sample surface are necessary. The high voltage…
Loading...
Imaging of Host Cell-bacteria Interactions using Correlative Microscopy under Cryo-conditions
Pathogenic bacteria have developed intriguing strategies to establish and promote infections in their respective hosts. Most bacterial pathogens initiate infectious diseases by adhering to host cells…
Loading...
冷冻断裂与冷冻蚀刻基础介绍
冷冻断裂是一种将冰冻样本劈裂以露出其内部结构的技术。冷冻蚀刻是指让样本表面的冰在真空中升华,以便露出原本无法观察到的断裂面细节。金属/碳复合镀膜能够实现样本在SEM(块面)或TEM(复型)中的成像,主要用于研究如细胞器、细胞膜,细胞层和乳胶。这项技术传统上用于生物学应用,但现在逐渐在物理学和材料科学中展现出重要意义。近年来,研究人员通过冷冻断裂电子显微镜,尤其是冷冻复型免疫标记(FRIL),对膜蛋…
Loading...
样本修块简介
使用超薄切片机制作样本超薄切片前需要预制样本。在预制过程中,必须特别留意样本尺寸(切片尺寸)、样本位置(目标定位)和块面边缘精准度。这个过程通常叫做修块,在此过程中样本大多被修块成金字塔截头锥体的形状。