Leica LMD6 & LMD7 레이저 미세절단 현미경 시스템
완벽한 해부
This list presents current publications in the major application fields for laser microdissection and is updated monthly.
Laser microdissection is a relatively new technique, having first been developed in the 1990s. It makes it possible to obtain homogenous, ultrapure samples from heterogenous starting material. A researcher can selectively and routinely analyze specific regions of interest, down to single cells, to obtain results that are reproducible. Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected via software, excised from the surrounding tissue by a laser, and collected by gravity into specialized devices for analysis.
The article by Mund et al. on Deep Visual Proteomics makes the cover of Nature Biotechnology for the month of August 2022.
Download the complete list of LMD publications in "Downloads"
Single Cell Applications
Deciphering functional tumor-immune crosstalk through highly multiplexed imaging and deep visual proteomics
Zheng X, Mund A, Mann M
Molecular Cell, 14 Jan 2025, ISSN 1097-2765, DOI: 10.1016/j.molcel.2024.12.023
Making space for spatial biology in the clinic
Nat Biotechnol (6 Jan2025).
https://doi.org/10.1038/s41587-024-02543-2
A new understanding of tissue biology from MS-based proteomics at single-cell resolution
Nordmann TM, Mund A, Mann M
Nat Methods 21, 2220–2222 (2024).
DNA Research
D-loop mutations in mitochondrial DNA are a risk factor for chemotherapy resistance in esophageal cancer
Harino T, Tanaka K, Motooka D, Masuike Y, Takahashi T, Yamashita K, Saito T, Yamamoto K, Makino T, Kurokawa Y, Nakajima K, Eguchi H, Doki Y
Sci Rep. 2024 Dec 30;14(1):31653. doi: 10.1038/s41598-024-80226-3.
High frequency of CTNNB1 variants associated with benign and malignant liver tumors in patients with Congenital Porto-Systemic Shunts
Tyraskis A, Zen Y, Strautnieks S, Cook R, Foskett P, De Vito C, Deheragoda M, Quaglia A, Heaton N, Davenport M, Thompson RJ
Liver Cancer 1–23. 28 Dec 2024, ISSN: 2235-1795 (Print), eISSN: 1664-5553, DOI: 10.1159/000543217
Cell of origin and expression profiles of pseudomyxoma peritonei derived from the appendix
Noguchi R, Yamaguchi K, Yano H, Gohda Y, Kiyomatsu T, Ota Y, Igari T, Takahashi N, Ohsugi T, Takane K, Ikenoue T, Niida A, Shimizu E, Yamaguchi R, Miyano S, Imoto S, Furukawa Y
Pathol Res Pract. 2024 Dec 21;266:155776. doi: 10.1016/j.prp.2024.155776.
RNA Research
GZMK-expressing CD8+ T cells promote recurrent airway inflammatory diseases
Lan F, Li J, Miao W, Sun F, Duan S, Song Y, Yao J, Wang X, Wang C, Liu X, Wang J, Zhang L, Qi H
Nature. 2025 Jan 15. doi: 10.1038/s41586-024-08395-9
A constitutive interferon-high immunophenotype defines response to immunotherapy in colorectal cancer
Acha-Sagredo A, Andrei P, Clayton K, Taggart E, Antoniotti C, Woodman CA, Afrache H, Fourny C, Armero M, Moinudeen HK, Green M, Bhardwaj N, Mikolajczak A, Rodriguez-Lopez M, Crawford M, Connick E, Lim S, Hobson P, Linares J, Ignatova E, Pelka D, Smyth EC, Diamantis N, Sosnowska D, Carullo M, Ciraci P, Bergamo F, Intini R, Nye E, Barral P, Mishto M, Arnold JN, Lonardi S, Cremolini C, Fontana E, Rodriguez-Justo M, Ciccarelli FD
Cancer Cell. 2025 Jan 13:S1535-6108(24)00483-5. doi: 10.1016/j.ccell.2024.12.008.
Chronic viral mimicry induction following p53 loss promotes immune evasion
Ishak CA, Marhon SA, Tchrakian N, Hodgson A, Loo Yau H, Gonzaga IM, Peralta M, Lungu IM, Gomez S, Liang SB, Shen SY, Chen R, Chen J, Chatterjee B, Wanniarachchi KN, Lee J, Zehrbach N, Hosseini A, Mehdipour P, Sun S, Solovyov A, Ettayebi I, Francis KE, He A, Wu T, Feng S, da Silva Medina T, Campos de Almeida F, Bayani J, Li J, MacDonald S, Wang Y, Garcia SS, Arthofer E, Diab N, Srivastava A, Austin PT, Sabatini PJB, Greenbaum BD, O'Brien CA, Shepherd TG, Tsao MS, Chiappinelli KB, Oza AM, Clarke BA, Rottapel R, Lheureux S, De Carvalho DD
Cancer Discov. 2025 Jan 7. doi: 10.1158/2159-8290.CD-24-0094.
Proteomics, Lipidomics, Metabolomics
Deciphering functional tumor-immune crosstalk through highly multiplexed imaging and deep visual proteomics
Zheng X, Mund A, Mann M
Molecular Cell, 14 Jan 2025, ISSN 1097-2765, DOI: 10.1016/j.molcel.2024.12.023
Multi-omics-based mapping of decidualization resistance in patients with a history of severe preeclampsia
Muñoz-Blat I, Pérez-Moraga R, Castillo-Marco N, Cordero T, Ochando A, Ortega-Sanchís S, Parras M, Monfort-Ortiz R, Satorres-Perez E, Novillo B, Perales A, Gormley M, Granados-Aparici S, Noguera R, Roson B, Fisher SJ, Simón C, Garrido-Gómez T
Nat Med. 2025 Jan 7. doi: 10.1038/s41591-024-03407-7.
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Highlight 2025:
Making space for spatial biology in the clinic
Nat Biotechnol (6 Jan2025).
https://doi.org/10.1038/s41587-024-02543-2
Highlights 2024:
Method of the Year 2024: spatial proteomics.
Nat Methods 21, 2195–2196 (2024).
https://doi.org/10.1038/s41592-024-02565-3
Nordmann et al.
https://www.nature.com/articles/s41586-024-08061-0
Highlights 2023: scDVP applied
Nordmann et al.
https://doi.org/10.1101/2023.11.11.23295492
Schweizer et al.
https://doi.org/10.1101/2023.11.13.23298409
Highlight 2022: Deep Visual Proteomics defines single-cell identity and heterogeneity
https://www.leica-microsystems.com/science-lab/life-science/precise-spatial-proteomic-information-in-tissues/
On single cell level:
Rosenberger FA et al. (scDVP):
https://doi.org/10.1038/s41592-023-02007-6
Original Paper:
Mund et al.
https://www.nature.com/articles/s41587-022-01302-5
Title page Nature Biotechnology, August 2022
https://www.nature.com/nbt/volumes/40/issues/8
Fluorescence Applications
Deciphering functional tumor-immune crosstalk through highly multiplexed imaging and deep visual proteomics
Zheng X, Mund A, Mann
Molecular Cell, 14 Jan 2025, ISSN 1097-2765, DOI: 10.1016/j.molcel.2024.12.023
The spatially informed mFISHseq assay resolves biomarker discordance and predicts treatment response in breast cancer
Paul ED, Huraiová B, Valková N, Matyasovska N, Gábrišová D, Gubová S, Ignačáková H, Ondris T, Gala M, Barroso L, Bendíková S, Bíla J, Buranovská K, Drobná D, Krchňáková Z, Kryvokhyzha M, Lovíšek D, Mamoilyk V, Mancikova V, Vojtaššáková N, Ristová M, Comino-Méndez I, Andrašina I, Morozov P, Tuschl T, Pareja F, Kather JN & Čekan P
Nat Commun 16, 226 (2025 Jan 2).
Publisher Correction: Novel laser model of optic nerve transection provides valuable insights about the dynamics of optic nerve regeneration
Moulin C, Dvoriantchikova G, Bineshfar N, Swingle B, Martinez G, Groso D, Zhang M, Ivanov D, Pelaez D
Sci Rep. 2024 Nov 29;14(1):29691. doi: 10.1038/s41598-024-80215-6.
Cancer Research
Epigenetic regulation of HOXA2 expression affects tumor progression and predicts breast cancer patient survival
De Palma FDE, Pol JG, Carbonnier V, Scuderi SA, Mannino D, Montégut L, Sauvat A, Perez-Lanzon M, Uribe-Carretero E, Guarracino M, Granata I, Calogero R, Del Monaco V, Montanaro D, Stoll G, Botti G, D'Aiuto M, Baldi A, D'Argenio V, Guigó R, Rezsohazy R, Kroemer G, Maiuri MC, Salvatore F
Cell Death Differ. 2025 Jan 20. doi: 10.1038/s41418-024-01430-2.
A constitutive interferon-high immunophenotype defines response to immunotherapy in colorectal cancer
Acha-Sagredo A, Andrei P, Clayton K, Taggart E, Antoniotti C, Woodman CA, Afrache H, Fourny C, Armero M, Moinudeen HK, Green M, Bhardwaj N, Mikolajczak A, Rodriguez-Lopez M, Crawford M, Connick E, Lim S, Hobson P, Linares J, Ignatova E, Pelka D, Smyth EC, Diamantis N, Sosnowska D, Carullo M, Ciraci P, Bergamo F, Intini R, Nye E, Barral P, Mishto M, Arnold JN, Lonardi S, Cremolini C, Fontana E, Rodriguez-Justo M, Ciccarelli FD
Cancer Cell. 2025 Jan 13:S1535-6108(24)00483-5. doi: 10.1016/j.ccell.2024.12.008.
Chronic viral mimicry induction following p53 loss promotes immune evasion
Ishak CA, Marhon SA, Tchrakian N, Hodgson A, Loo Yau H, Gonzaga IM, Peralta M, Lungu IM, Gomez S, Liang SB, Shen SY, Chen R, Chen J, Chatterjee B, Wanniarachchi KN, Lee J, Zehrbach N, Hosseini A, Mehdipour P, Sun S, Solovyov A, Ettayebi I, Francis KE, He A, Wu T, Feng S, da Silva Medina T, Campos de Almeida F, Bayani J, Li J, MacDonald S, Wang Y, Garcia SS, Arthofer E, Diab N, Srivastava A, Austin PT, Sabatini PJB, Greenbaum BD, O'Brien CA, Shepherd TG, Tsao MS, Chiappinelli KB, Oza AM, Clarke BA, Rottapel R, Lheureux S, De Carvalho DD
Cancer Discov. 2025 Jan 7. doi: 10.1158/2159-8290.CD-24-0094.
Neurosciences
Persistence of spike protein at the skull-meninges-brain axis may contribute to the neurological sequelae of COVID-19
Rong Z, Mai H, Ebert G, Kapoor S, Puelles VG, Czogalla J, Hu S, Su J, Prtvar D, Singh I, Schädler J, Delbridge C, Steinke H, Frenzel H, Schmidt K, Braun C, Bruch G, Ruf V, Ali M, Sühs KW, Nemati M, Hopfner F, Ulukaya S, Jeridi D, Mistretta D, Caliskan ÖS, Wettengel JM, Cherif F, Kolabas ZI, Molbay M, Horvath I, Zhao S, Krahmer N, Yildirim AÖ, Ussar S, Herms J, Huber TB, Tahirovic S, Schwarzmaier SM, Plesnila N, Höglinger G, Ondruschka B, Bechmann I, Protzer U, Elsner M, Bhatia HS, Hellal F, Ertürk A
Cell Host Microbe. 2024 Nov 26:S1931-3128(24)00438-4. doi: 10.1016/j.chom.2024.11.007.
Deep RNA sequencing of human dorsal root ganglion neurons reveals somatosensory mechanisms
Yu H, Luo W
Nat Neurosci (6 Nov 2024).
Leveraging deep single-soma RNA sequencing to explore the neural basis of human somatosensation
Yu H, Nagi SS, Usoskin D, Hu Y, Kupari J, Bouchatta O, Yan H, Cranfill SL, Gautam M, Su Y, Lu Y, Wymer J, Glanz M, Albrecht P, Song H, Ming GL, Prouty S, Seykora J, Wu H, Ma M, Marshall A, Rice FL, Li M, Olausson H, Ernfors P, Luo W
Nat Neurosci. 2024 Nov 4. doi: 10.1038/s41593-024-01794-1.
Clinical/Pathology/Immunology Research Applications
GZMK-expressing CD8+ T cells promote recurrent airway inflammatory diseases
Lan F, Li J, Miao W, Sun F, Duan S, Song Y, Yao J, Wang X, Wang C, Liu X, Wang J, Zhang L, Qi H
Nature. 2025 Jan 15. doi: 10.1038/s41586-024-08395-9
Multi-omics-based mapping of decidualization resistance in patients with a history of severe preeclampsia
Muñoz-Blat I, Pérez-Moraga R, Castillo-Marco N, Cordero T, Ochando A, Ortega-Sanchís S, Parras M, Monfort-Ortiz R, Satorres-Perez E, Novillo B, Perales A, Gormley M, Granados-Aparici S, Noguera R, Roson B, Fisher SJ, Simón C, Garrido-Gómez T
Nat Med. 2025 Jan 7. doi: 10.1038/s41591-024-03407-7.
Complement classical and alternative pathway activation contributes to diabetic kidney disease progression: a glomerular proteomics on kidney biopsies
Yang Y, Zhang Y, Li Y, Zhou X, Honda K, Kang D, Wang M, Yang JH, Xia Z, Wei Y, Liu L, Hu R, Takaki T, Xing G
Sci Rep. 2025 Jan 2;15(1):495. doi: 10.1038/s41598-024-84900-4.
Developmental Research, Epigenetics & Aging
Multi-omics-based mapping of decidualization resistance in patients with a history of severe preeclampsia
Muñoz-Blat I, Pérez-Moraga R, Castillo-Marco N, Cordero T, Ochando A, Ortega-Sanchís S, Parras M, Monfort-Ortiz R, Satorres-Perez E, Novillo B, Perales A, Gormley M, Granados-Aparici S, Noguera R, Roson B, Fisher SJ, Simón C, Garrido-Gómez T
Nat Med. 2025 Jan 7. doi: 10.1038/s41591-024-03407-7.
Age-related TFEB downregulation in proximal tubules causes systemic metabolic disorders and occasional apolipoprotein A4-related amyloidosis
Nakamura J, Yamamoto T, Takabatake Y, Namba-Hamano T, Takahashi A, Matsuda J, Minami S, Sakai S, Yonishi H, Maeda S, Matsui S, Kawai H, Matsui I, Yamamuro T, Edahiro R, Takashima S, Takasawa A, Okada Y, Yoshimori T, Ballabio A, Isaka
JCI Insight. 2024 Dec 19:e184451. doi: 10.1172/jci.insight.184451.
Spatiotemporal functions of leukemia inhibitory factor in embryo attachment and implantation chamber formation
Aikawa S, Hiraoka T, Matsuo M, Fukui Y, Fujita H, Saito-Fujita T, Shimizu-Hirota R, Takeda N, Hiratsuka D, He X, Ishizawa C, Iida R, Akaeda S, Harada M, Wada-Hiraike O, Ikawa M, Osuga Y, Hirota Y
Cell Death Discov. 2024 Nov 25;10(1):481. doi: 10.1038/s41420-024-02228-4.
Plant Research
RNA Extraction from Rice Immature Embryo Using Laser Capture Microdissection
Tezuka T, Nosaka-Takahashi M, Yoshikawa T, Sato Y
Methods in Molecular Biology, vol 2869. (2025) in: Kawakatsu, T. (eds) Rice.
Dissecting the cellular architecture and genetic circuitry of the soybean seed
Pelletier JM, Chen M, Lin J-Y, Le B, Kirkbride RC, Hur J, Wang T, Chang S-H, Olsona A, Nikolov L, Goldberg RB, and Harada JJ
PNAS, 30 dec 2024, Vol. 122, No. 1, e2416987121
XYLAN O-ACETYLTRANSFERASE 6 promotes xylan synthesis by forming a complex with IRX10 and governs wall formation in rice
Wen Z, Xu Z, Zhang L, Xue Y, Wang H, Jian L, Ma J, Liu Z, Yang H, Huang S, Kang X, Zhou Y, Zhang B
Plant Cell. 2024 Dec 23;37(1):koae322. doi: 10.1093/plcell/koae322.
Forensics
Advancements in differentiation between sperm cells and epithelial cells for efficient forensic DNA analysis in sexual assault cases
Dash HR
Int J Legal Med. 2024 Jul 12. doi: 10.1007/s00414-024-03285-1.
Development of a Simple Cell Harvesting Method to Maximise DNA Recovery from Historic Microscope Slides for Sexual Assault Investigations
Hymus CM, Egan SE and Tay JW
Forensic Sci. 2022, 2(4), 795-807
Population Genetic Issues for Forensic DNA Profiles
Rhodes C
NCJRS, Document Number: 304612, Date Received: April 2022, Award Number: 2017-DN-BX-0136
General Information:
http://projects.nfstc.org/tech_transition/lmd/examiners/
Miscellaneous
Aβ40 Fibril Assembly on Human Cerebral Smooth Muscle Cells Impairs Cell Viability
Irizarry B, Davis J, Rajpoot J, Zhu X, Xu F, Smith SO, Van Nostrand WE
Biochemistry. 2025 Jan 6. doi: 10.1021/acs.biochem.4c00478.
Methods for Selective Gene Expression Profiling in Single Tertiary Lymphoid Structure Using Laser Capture Microdissection
Gutierrez-Chavez, C., Knockaert, S., Dieu-Nosjean, MC., Goc, J.
Dieu-Nosjean, MC. (eds) Tertiary Lymphoid Structures. Methods in Molecular Biology, vol 2864. Humana, New York, NY (2025).
Laser Capture Microdissection of Tertiary Lymphoid Structures from Formalin-Fixed Paraffin-Embedded Sections of Canine Cutaneous and Subcutaneous Sarcomas for NanoString Direct RNA Counting
Olver, C.S.
Dieu-Nosjean, MC. (eds) Tertiary Lymphoid Structures. Methods in Molecular Biology, vol 2864. Humana, New York, NY (2025).
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Highlight:
Multiple: Laser Microdissection Protocols for Tissue and Cell Isolation
Bio-protocol Selections | 2024
https://en.bio-protocol.org/ebookinfo.aspx?id=23
Live Cell Culture
A 3‑Dimensional Scaffolding System Recapitulates the Hierarchical Osteon Structure
Li X, Sun Y, Wang S, Si C, Li H, and Chang B
ACS Omega 2024, XXXX, XXX, XXX-XXX
A novel method for the isolation of single cells mimicking circulating tumour cells adhered on Smart Bio Surface slides by Laser Capture Microdissection
Visci G, Tolomeo D, Lonoce A, Arshadi A, Bascetta L, Trotta G, van Riel M, Vermeesch JR, Carbone R, Storlazzi CT
Protocols.io, 26 Oct 2023
SPARCS, a platform for genome-scale CRISPR screening for spatial cellular phenotypes
Schmacke NA, Mädler SC, Wallmann G, Metousis A, Bérouti M, Harz H, Leonhardt H, Mann M, Horning V
bioRxiv, 1 June 2023, DOI: 10.1101/2023.06.01.542416