Leica LMD6 & LMD7 정립 현미경 광학 현미경 제품소개 Leica Microsystems

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레이저 미세절단 현미경 시스템 Leica LMD6 & LMD7

This list presents current publications in the major application fields for laser microdissection and is updated monthly.

Laser microdissection is a relatively new technique, having first been developed in the 1990s. It makes it possible to obtain homogenous, ultrapure samples from heterogenous starting material. A researcher can selectively and routinely analyze specific regions of interest, down to single cells, to obtain results that are reproducible. Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected via software, excised from the surrounding tissue by a laser, and collected by gravity into specialized devices for analysis.

Download the complete list of LMD publications in "Downloads"

DNA Research

Mutational landscape of normal epithelial cells in Lynch Syndrome patients
Lee BCH, Robinson PS, Coorens THH, Yan HHN, Olafsson S, Lee-Six H, Sanders MA, Siu HC, Hewinson J, Yue SSK, Tsui WY, Chan ASY, Chan AKW, Ho SL, Campbell PJ, Martincorena I, Buczacki SJA, Yuen ST, Leung SY, Stratton MR
Nat Commun. 2022 May 17;13(1):2710. doi: 10.1038/s41467-022-29920-2.

Low mitochondrial DNA copy number induces chemotherapy resistance via epithelialmesenchymal transition by DNA methylation in esophageal squamous cancer cells
Kubo Y, Tanaka K, Masuike Y, Yamashita K, Makino T, Saito T, Yamamoto K, Takahashi T, Kurokawa Y, Yamasaki M, Nakajima K, Eguchi H, Doki Y
Research Square, 6th May 2022, DOI: doi.org/10.21203/rs.3.rs-1602834/v1

Transcriptomic profiles of neoantigen-reactive T cells in human gastrointestinal cancers
Zheng C, Fass JN, Shih YP, Gunderson AJ, Sanjuan Silva N, Huang H, Bernard BM, Rajamanickam V, Slagel J, Bifulco CB, Piening B, Newell PHA, Hansen PD, Tran E
Cancer Cell. 2022 Apr 11;40(4):410-423.e7. doi: 10.1016/j.ccell.2022.03.005.

RNA Research

Circadian protection against bacterial skin infection by epidermal CXCL14-mediated innate immunity
Tsujihana K, Tanegashima K, Santo Y, Yamada H, Akazawa S, Nakao R, Tominaga K, Saito R, Nishito Y, Hata RI, Nakamura T, Murai I, Kono Y, Sugawa M, Tanioka M, Egawa G, Doi M, Isa T, Kabashima K, Hara T, Okamura H
Proc Natl Acad Sci U S A. 2022 Jun 21;119(25):e2116027119. doi: 10.1073/pnas.2116027119. Epub 2022 Jun 15.

Gene expression signatures of individual ductal carcinoma in situ lesions identify processes and biomarkers associated with progression towards invasive ductal carcinoma
Rebbeck CA, Xian J, Bornelöv S, Geradts J, Hobeika A, Geiger H, Alvarez JF, Rozhkova E, Nicholls A, Robine N, Lyerly HK, Hannon GJ
Nat Commun. 2022 Jun 13;13(1):3399. doi: 10.1038/s41467-022-30573-4.

Activation of CD44 signaling in leader cells induced by tumor-associated macrophages drives collective detachment in luminal breast carcinomas
Gao F, Zhang G, Liu Y, He Y, Sheng Y, Sun X, Du Y, Yang C
Cell Death Dis. 2022 Jun 9;13(6):540. doi: 10.1038/s41419-022-04986-4.

Proteomics, Lipidomics, Metabolomics

Unbiased spatial proteomics with single-cell resolution in tissues
Mund A, Brunner AD, Mann M
Mol Cell. 2022 Jun 16;82(12):2335-2349. doi: 10.1016/j.molcel.2022.05.022.

Industrialized, Artificial Intelligence-guided Laser Microdissection for Microscaled Proteomic Analysis of the Tumor Microenvironment
Mitchell, D., Hunt, A. L., Conrads, K. A., Hood, B. L., Makohon-Moore, S. C., Rojas, C., Maxwell, G. L., Bateman, N. W., Conrads, T. P.
J. Vis. Exp. (184), e64171, doi:10.3791/64171 (2022).

Deep Visual Proteomics defines single-cell identity and heterogeneity
Mund A, Coscia F, Kriston A, Hollandi R, Kovacs F, Brunner A-D, Migh E, Schweizer L, Santos A, Bzorek M, Naimy S, Rahbeck Gjerdum LM, Dyring-Andersen B, Bulkescher JM, Lukas C, Gnann C, Lundberg E, Horvath P, Mann M
Nat Biotechnol (2022).

Fluorescence Applications

Mechanical forces couple bone matrix mineralization with inhibition of angiogenesis to limit adolescent bone growth
Dzamukova M, Brunner TM, Miotla-Zarebska J, Heinrich F, Brylka L, Mashreghi MF, Kusumbe A, Kühn R, Schinke T, Vincent TL, Löhning M
Mechanical forces couple bone matrix mineralization with inhibition of angiogenesis to limit adolescent bone growth
Nat Commun. 2022 Jun 1;13(1):3059. doi: 10.1038/s41467-022-30618-8.

Lower Levels of GABAergic Function Markers in Corticotropin-Releasing Hormone-Expressing Neurons in the sgACC of Human Subjects With Depression
Oh H, Newton D, Lewis D, Sibille E:
Front Psychiatry. 2022 Feb 25;13:827972. doi: 10.3389/fpsyt.2022.827972. eCollection 2022.

The Amyloid Plaque Proteome in Early Onset Alzheimer’s Disease and Down Syndrome
Drummond E, Kavanagh T, Pires G, Ariza MM, Kanshin E, Nayak S, Faustin A, Berdah V, Ueberheide B, Wisniewski T:
Resrearch Square, 8 Feb 2022, doi: 10.21203/rs.3.rs-1316035/v1

Cancer Research

Gene expression signatures of individual ductal carcinoma in situ lesions identify processes and biomarkers associated with progression towards invasive ductal carcinoma
Rebbeck CA, Xian J, Bornelöv S, Geradts J, Hobeika A, Geiger H, Alvarez JF, Rozhkova E, Nicholls A, Robine N, Lyerly HK, Hannon GJ
Nat Commun. 2022 Jun 13;13(1):3399. doi: 10.1038/s41467-022-30573-4.

Activation of CD44 signaling in leader cells induced by tumor-associated macrophages drives collective detachment in luminal breast carcinomas
Gao F, Zhang G, Liu Y, He Y, Sheng Y, Sun X, Du Y, Yang C
Cell Death Dis. 2022 Jun 9;13(6):540. doi: 10.1038/s41419-022-04986-4.

Mutational landscape of normal epithelial cells in Lynch Syndrome patients
Lee BCH, Robinson PS, Coorens THH, Yan HHN, Olafsson S, Lee-Six H, Sanders MA, Siu HC, Hewinson J, Yue SSK, Tsui WY, Chan ASY, Chan AKW, Ho SL, Campbell PJ, Martincorena I, Buczacki SJA, Yuen ST, Leung SY, Stratton MR
Nat Commun. 2022 May 17;13(1):2710. doi: 10.1038/s41467-022-29920-2.

Neurosciences

Distinct gene expression in demyelinated white and grey matter areas of patients with multiple sclerosis
van Wageningen TA, Gerrits E, Brouwer N, Brevé JJP, Geurts JJG, Eggen BJL, Boddeke HWGME, van Dam AM
Brain Commun. 2022 Jan 17;4(2):fcac005. doi: 10.1093/braincomms/fcac005. eCollection 2022.

Transcriptome Analysis of Schwann Cells at Various Stages of Myelination Implicates Chromatin Regulator Sin3A in Control of Myelination Identity
Zhang B, Su W, Hu J, Xu J, Askar P, Bao S, Zhou S, Chen G, Gu Y
Neurosci Bull. 2022 Apr 10. doi: 10.1007/s12264-022-00850-9.

Interplay between autophagy and amyloid beta metabolism in Alzheimer’s disease
Jiang R
Thesis, 2022

Clinical/Pathology/Immunology Research Applications

Circadian protection against bacterial skin infection by epidermal CXCL14-mediated innate immunity
Tsujihana K, Tanegashima K, Santo Y, Yamada H, Akazawa S, Nakao R, Tominaga K, Saito R, Nishito Y, Hata RI, Nakamura T, Murai I, Kono Y, Sugawa M, Tanioka M, Egawa G, Doi M, Isa T, Kabashima K, Hara T, Okamura H
Proc Natl Acad Sci U S A. 2022 Jun 21;119(25):e2116027119. doi: 10.1073/pnas.2116027119. Epub 2022 Jun 15.

Pattern of tamoxifen-induced Tie2 deletion in endothelial cells in mature blood vessels using endo SCL-Cre-ERT transgenic mice
Zwiers PJ, Jongman RM, Kuiper T, Moser J, Stan RV, Göthert JR, van Meurs M, Popa ER, Molema G
PLoS One. 2022 Jun 8;17(6):e0268986. doi: 10.1371/journal.pone.0268986. eCollection 2022.

Misaligned feeding schedule elicits divergent circadian reorganizations in endo- and exocrine pancreas clocks
Honzlová P, Novosadová Z, Houdek P, Sládek M, Sumová A
Cell Mol Life Sci. 2022 May 27;79(6):318. doi: 10.1007/s00018-022-04354-7.

Developmental Research, Epigenetics  & Aging

Mechanical forces couple bone matrix mineralization with inhibition of angiogenesis to limit adolescent bone growth
Dzamukova M, Brunner TM, Miotla-Zarebska J, Heinrich F, Brylka L, Mashreghi MF, Kusumbe A, Kühn R, Schinke T, Vincent TL, Löhning M
Nat Commun. 2022 Jun 1;13(1):3059. doi: 10.1038/s41467-022-30618-8.

Symbiosis with Dinoflagellates Alters Cnidarian Cell-Cycle Gene Expression
Gorman LM, Konciute MK, Cui G, Oakley CA, Grossman AR, Weis VM, Aranda M, and Davy SK
Cellular Microbiology, (23rd May) Volume 2022, Article ID 3330160, 20 pages

Laser Microdissection for Species-agnostic Single-tissue Applications
Woronik A, Kiontke K, Jallad RS, Herrera RA, Fitch DHA
J Vis Exp. 2022 Mar 31;(181). doi: 10.3791/63666.

Plant Research

An amino acid transporter-like protein (OsATL15) facilitates the systematic distribution of thiamethoxam in rice for controlling the brown planthopper
Xiao Y, Zhang H, Li Z, Huang T, Akihiro T, Xu J, Xu H, Lin F
Plant Biotechnol J. 2022 Jun 9. doi: 10.1111/pbi.13869.

Narrow lpa1 Metaxylems Enhance Drought Tolerance and Optimize Water Use for Grain Filling in Dwarf Rice
Priatama RA, Heo J, Kim SH, Rajendran S, Yoon S, Jeong D-H, Choo Y-K, Bae JH, Kim CM, Lee YH, Demura T, Lee YK, Choi E-Y, Han C-d and Park SJ
Front. Plant Sci. 13:894545. (10 May 2022), doi: 10.3389/fpls.2022.894545

Morphogenesis, ultrastructure, and chemical profiling of trichomes in Artemisia argyi H. Lév. & Vaniot (Asteraceae)
Cui, Z., Li, M., Han, X., Liu, H., Li, C., Peng, H., Liu, D., Huang, X., and Zhang, Z.
Planta 255, 102 (2022).

Forensics

Population Genetic Issues for Forensic DNA Profiles
Rhodes C
NCJRS, Document Number: 304612, Date Received: April 2022, Award Number: 2017-DN-BX-0136

Sperm Cell Capture Based on ABH Antigen Differences to Separate Two Men in Mixed Seminal Stains
Sun M, Zheng J, Wnag B and Yao J:
BioMed Research International, Volume 2021 | Article ID 7269237

Application and utility of alternative methods in isolation of pure cells from forensic biological mixtures in modern-day: a review
Prajapati S, Rajmane P, Jayakrishna P, Nair MS, Kshirsagar P and Meshram M:
JFSR – Journal of Forensic Science and Research, Aug 2021, ISSN 2575-0186

General Information:
http://projects.nfstc.org/tech_transition/lmd/examiners/

Further information on Leica LMD impact at forensics: 
http://www.fox23.com/news/news/local/new-dna-lab-brings-hope-victims-families/nj97Z/

Miscellaneous

Unbiased spatial proteomics with single-cell resolution in tissues
Mund A, Brunner AD, Mann M
Mol Cell. 2022 Jun 16;82(12):2335-2349. doi: 10.1016/j.molcel.2022.05.022.

Industrialized, Artificial Intelligence-guided Laser Microdissection for Microscaled Proteomic Analysis of the Tumor Microenvironment
Mitchell, D., Hunt, A. L., Conrads, K. A., Hood, B. L., Makohon-Moore, S. C., Rojas, C., Maxwell, G. L., Bateman, N. W., Conrads, T. P.
J. Vis. Exp. (184), e64171, doi:10.3791/64171 (2022).

Electrical sensor system for in vitro bacteria biofilm diagnostics
Guliy OI, Evstigneeva SS, and Bunin VD
Biosensors and Bioelectronics: X (2022 May 23rd), PII: S2590-1370(22)00069-3

Live Cell Culture

Activation of CD44 signaling in leader cells induced by tumor-associated macrophages drives collective detachment in luminal breast carcinomas
Gao F, Zhang G, Liu Y, He Y, Sheng Y, Sun X, Du Y, Yang C
Cell Death Dis. 2022 Jun 9;13(6):540. doi: 10.1038/s41419-022-04986-4.

Transcriptome Analysis of Schwann Cells at Various Stages of Myelination Implicates Chromatin Regulator Sin3A in Control of Myelination Identity
Zhang B, Su W, Hu J, Xu J, Askar P, Bao S, Zhou S, Chen G, Gu Y
Neurosci Bull. 2022 Apr 10. doi: 10.1007/s12264-022-00850-9.

Insight into the Molecular Characteristics of Langhans Giant Cell by Combination of Laser Capture Microdissection and RNA Sequencing
Chen Y, Jiang H, Xiong J, Shang J, Chen Z, Wu A, Wang H:
Journal of Inflammation Research, 2 Feb 2022,  Vol. 15, doi: 10.2147/JIR.S337241