Leica LMD6 & LMD7 레이저 미세절단 현미경 시스템

This list presents current publications in the major application fields for laser microdissection and is updated monthly.

Laser microdissection is a relatively new technique, having first been developed in the 1990s. It makes it possible to obtain homogenous, ultrapure samples from heterogenous starting material. A researcher can selectively and routinely analyze specific regions of interest, down to single cells, to obtain results that are reproducible. Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected via software, excised from the surrounding tissue by a laser, and collected by gravity into specialized devices for analysis.

The article by Mund et al. on Deep Visual Proteomics makes the cover of Nature Biotechnology for the month of August 2022.

Download the complete list of LMD publications in "Downloads"

Single Cell Applications

Multiplexed Deep Visual Proteomics Unveils Spatial Heterogeneity and Rare Endocrine States in Human Adult Pancreatic Islets
Mardamshina M, Dorka N, Thielert M, Bjoerklund F, Navarro FB, Casals AM, Kovacs F, Fox JEM, Horvath P, Macdonald PE, Mann M, Lundberg
bioRxiv 27 April 2025

Mass Spectrometry-Based Proteomics for Next-Generation Precision Oncology
Lin KT, Muneer G, Huang PR, Chen CS, Chen YJ
Mass Spectrom Rev. 2025 Apr 23. doi: 10.1002/mas.21932.

Deep Visual Proteomics maps proteotoxicity in a genetic liver disease
Rosenberger FA, Mädler SC, Thorhauge KH, Steigerwald S, Fromme M, Lebedev M, Weiss CAM, Oeller M, Wahle M, Metousis A, Zwiebel M, Schmacke NA, Detlefsen S, Boor P, Fabián O, Fraňková S, Krag A, Strnad P, Mann M
Nature. 2025 Apr 16. doi: 10.1038/s41586-025-08885-4.

DNA Research

Clinicopathological and molecular characterization of KRAS wild-type pancreatic ductal adenocarcinomas reveals precursor lesions with oncogenic mutations and fusions in RAS pathway genes
Toriyama K, Masago K, Shibata N, Haneda M, Kuwahara T, Natsume S, Kobayashi S, Fujita Y, Sasaki E, Yamao K, Kawashima H, Shimizu Y, Hara K, Yatabe Y, Hosoda W
J Pathol. 2025 May 2. doi: 10.1002/path.6432.

Two-hit events occurred independently in bilateral breast cancer of a heterozygous double germline pathogenic variants carrier of BRCA1 and BRCA2
Semba R, Eguchi H, Takatsu M, Hashizume T, Moteki H, Maeno K, Murakami F, Watanabe J, Kutomi G, Arai M
Research Square, 1 Apr 2025

Selection for somatic escape variants in SERPINA1 in the liver of patients with alpha-1 antitrypsin deficiency
Brzozowska N, Wu LYD, Khodzhaeva V, Griffiths WJ, Duckworth A, Jung H, Coorens THH, Hooks Y, Chambers JE, Campbell PJ, Marciniak SJ, Hoare M
Nat Genet. 2025 Mar 10. doi: 10.1038/s41588-025-02125-1.

RNA Research

Single-cell genotyping and transcriptomic profiling of mosaic focal cortical dysplasia
Baldassari S, Klingler E, Teijeiro LG, Doladilhe M, Raoux C, Roig-Puiggros S, Bizzotto S, Couturier J, Gilbert A, Sami L, Ribierre T, Aronica E, Adle-Biassette H, Chipaux M, Jabaudon D, Baulac S
Nat Neurosci. 2025 Apr 30. doi: 10.1038/s41593-025-01936-z.

Interactions of CFTR and Arylsulfatase B (ARSB;Nacetylgalactosamine-4-sulfatase) in Prostate Carcinoma
Bhattacharyya S, Tobacman J
preprint, 21. Apr 2025, doi: 10.20944/preprints202504.1708.v1

Transcriptome Meta-Analysis Uncovers Cell-Specific Regulatory Relationships in Embryonic, Juvenile, Adult, and Aged Mouse Lens Epithelium and Fibers
Duot M, Coomson SY, Shrestha SK, Nagulla MVMK, Audic Y, Barve RA, Huang H, Gautier-Courteille C, Paillard L, Lachke SA
Invest Ophthalmol Vis Sci. 2025 Apr 1;66(4):42. doi: 10.1167/iovs.66.4.42.

Proteomics, Lipidomics, Metabolomics

Multiplexed Deep Visual Proteomics Unveils Spatial Heterogeneity and Rare Endocrine States in Human Adult Pancreatic Islets
Mardamshina M, Dorka N, Thielert M, Bjoerklund F, Navarro FB, Casals AM, Kovacs F, Fox JEM, Horvath P, Macdonald PE, Mann M, Lundberg
bioRxiv 27 April 2025

Spatial proteomics in translational and clinical research
Horvath P, Coscia F
Mol Syst Biol. 2025 Apr 14. doi: 10.1038/s44320-025-00101-9

Deep Visual Proteomics maps proteotoxicity in a genetic liver disease
Rosenberger FA, Mädler SC, Thorhauge KH, Steigerwald S, Fromme M, Lebedev M, Weiss CAM, Oeller M, Wahle M, Metousis A, Zwiebel M, Schmacke NA, Detlefsen S, Boor P, Fabián O, Fraňková S, Krag A, Strnad P, Mann M
Nature. 2025 Apr 16. doi: 10.1038/s41586-025-08885-4.

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Highlight 2025: 

Rosenberger FA, et al.
Nature (2025 Apr 16)
https://www.nature.com/articles/s41586-025-08885-4

Making space for spatial biology in the clinic
Nat Biotechnol (6 Jan2025).
https://doi.org/10.1038/s41587-024-02543-2

Highlights 2024: 

Method of the Year 2024: spatial proteomics. 
Nat Methods 21, 2195–2196 (2024). 
https://doi.org/10.1038/s41592-024-02565-3
Nordmann et al.
https://www.nature.com/articles/s41586-024-08061-0

Highlights 2023: scDVP applied
Nordmann et al.
https://doi.org/10.1101/2023.11.11.23295492
Schweizer et al.
https://doi.org/10.1101/2023.11.13.23298409

Highlight 2022: Deep Visual Proteomics defines single-cell identity and heterogeneity
https://www.leica-microsystems.com/science-lab/life-science/precise-spatial-proteomic-information-in-tissues/
On single cell level:
Rosenberger FA et al. (scDVP):
https://doi.org/10.1038/s41592-023-02007-6
Original Paper:
Mund et al.
https://www.nature.com/articles/s41587-022-01302-5
Title page Nature Biotechnology, August 2022
https://www.nature.com/nbt/volumes/40/issues/8

Fluorescence Applications

Multiplexed Deep Visual Proteomics Unveils Spatial Heterogeneity and Rare Endocrine States in Human Adult Pancreatic Islets
Mardamshina M, Dorka N, Thielert M, Bjoerklund F, Navarro FB, Casals AM, Kovacs F, Fox JEM, Horvath P, Macdonald PE, Mann M, Lundberg
bioRxiv 27 April 2025

Deep Visual Proteomics maps proteotoxicity in a genetic liver disease
Rosenberger FA, Mädler SC, Thorhauge KH, Steigerwald S, Fromme M, Lebedev M, Weiss CAM, Oeller M, Wahle M, Metousis A, Zwiebel M, Schmacke NA, Detlefsen S, Boor P, Fabián O, Fraňková S, Krag A, Strnad P, Mann M
Nature. 2025 Apr 16. doi: 10.1038/s41586-025-08885-4.

Deep spatial proteomics of ovarian cancer precursor lesions delineates early disease changes and cell-of-origin signatures
Makhmut A, Dragomir MP, Fritzsche S, Moebs M, Schmitt WD, Taube ET, and Coscia F
bioRxiv, 19 Mar 2025

Cancer Research

Clinicopathological and molecular characterization of KRAS wild-type pancreatic ductal adenocarcinomas reveals precursor lesions with oncogenic mutations and fusions in RAS pathway genes
Toriyama K, Masago K, Shibata N, Haneda M, Kuwahara T, Natsume S, Kobayashi S, Fujita Y, Sasaki E, Yamao K, Kawashima H, Shimizu Y, Hara K, Yatabe Y, Hosoda W
J Pathol. 2025 May 2. doi: 10.1002/path.6432.

AI-powered deep visual proteomics (DVP) for early pancreatic cancer insights 
Min J, Schweizer L, Zonderland G, Selvanesan BC, Oldenburg L, Hollingsworth MA, Grandgenett PM, Ummat I, Strauss MT, Mund A, Maitra A
Proceedings of the American Association for Cancer Research Annual Meeting 2025; Part 1 (Regular Abstracts); 2025 Apr 25-30; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2025;85(8_Suppl_1):Abstract nr 753.

Histology-resolved proteomics reveals distinct tumor and stromal profiles in low- and high-grade prostate cancer
Hunt AL, Barakat W, Makohon-Moore SC, Hood BL, Conrads KA, Wilson KN, Abulez T, Ogata J, Pienta KJ, Lotan TL, Mani H, Trump DL, Bateman NW, Conrads TP
Clin Proteomics. 2025 Apr 20;22(1):14. doi: 10.1186/s12014-025-09534-8.

Neurosciences

Single-cell genotyping and transcriptomic profiling of mosaic focal cortical dysplasia
Baldassari S, Klingler E, Teijeiro LG, Doladilhe M, Raoux C, Roig-Puiggros S, Bizzotto S, Couturier J, Gilbert A, Sami L, Ribierre T, Aronica E, Adle-Biassette H, Chipaux M, Jabaudon D, Baulac S
Nat Neurosci. 2025 Apr 30. doi: 10.1038/s41593-025-01936-z.

Neural tube defects induce abnormal astrocyte development by activation and epigenetic permissiveness of STAT3
Janik K, Jin LQ, Kyzy KZ, Kaminski R, Smith GM, Krynska B
Exp Neurol. 2025 Mar 30;389:115231. doi: 10.1016/j.expneurol.2025.115231.

In-depth and high-throughput spatial proteomics for whole-tissue slice profiling by deep learning-facilitated sparse sampling strategy
Qin R, Ma J, He F, Qin W
Cell Discov 11, 21 (2025). 

Clinical/Pathology/Immunology Research Applications

Proteomic Insights into IgA Nephropathy: A Comprehensive Analysis of Differentially Expressed Proteins in the Kidney
Huang J, Zhu H, Li S, Qiu J, Yang H, Zheng F, Luo Z, Yan Q, Liu F, Yin L, Tang D, and Dai Y
ACS Omega 2025 Apr 24, XXXX, XXX, XXX-XXX

Spatial proteomics in translational and clinical research
Horvath P, Coscia F
Mol Syst Biol. 2025 Apr 14. doi: 10.1038/s44320-025-00101-9

Deep Visual Proteomics maps proteotoxicity in a genetic liver disease
Rosenberger FA, Mädler SC, Thorhauge KH, Steigerwald S, Fromme M, Lebedev M, Weiss CAM, Oeller M, Wahle M, Metousis A, Zwiebel M, Schmacke NA, Detlefsen S, Boor P, Fabián O, Fraňková S, Krag A, Strnad P, Mann 
Nature. 2025 Apr 16. doi: 10.1038/s41586-025-08885-4.

Developmental Research, Epigenetics & Aging

Transcriptome Meta-Analysis Uncovers Cell-Specific Regulatory Relationships in Embryonic, Juvenile, Adult, and Aged Mouse Lens Epithelium and Fibers
Duot M, Coomson SY, Shrestha SK, Nagulla MVMK, Audic Y, Barve RA, Huang H, Gautier-Courteille C, Paillard L, Lachke SA
Invest Ophthalmol Vis Sci. 2025 Apr 1;66(4):42. doi: 10.1167/iovs.66.4.42.

Integrin α11β1 as a Key Collagen Receptor in Human Skin Dermis: Insight into Fibroblast Function and Skin Dermal Aging
Quan T, Qin Z, He T, Fisher GJ
J Invest Dermatol. 2025 Mar 24:S0022-202X(25)00364-1. doi: 10.1016/j.jid.2025.03.006

Cancer-independent somatic mutation of the wild-type NF1 allele in normal tissues in neurofibromatosis type 1
Oliver TRW, Lawson ARJ, Lee-Six H, Tollit A, Jung H, Hooks Y, Sanghvi R, Young MD, Butler TM, Nicola PA, Treger TD, Lensing SV, Burke GAA, Aquilina K, Löbel U, Cortes-Ciriano I, Hargrave D, Jorgensen M, Jessop FA, Coorens THH, Flanagan AM, Allinson K, Martincorena I, Jacques TS, Behjati S
Nat Genet. 2025 Feb 25. doi: 10.1038/s41588-025-02097-2.

Plant Research

Laser microdissection and fluorescence in situ hybridization reveal the tissue-specific gene expression in the ovules of P. tabulaeformis Carr
Zhang X, Chen B, Song X, Wang Y, Zheng C, Gong Z
J Plant Physiol. 2025 Apr 22:309:154500. doi: 10.1016/j.jplph.2025.154500.

Role of an endodermis-specificmiR858b-MYB1Lmodule inthe regulation of Taxol biosynthesis inTaxus mairei
Yu C, Zhang D, Zhang L, Fang Z, Zhang Y, Lin W, Ma R, Zheng M, Bai E, Shen
The plant journal, Volume122, Issue1, 05 April 2025, e70135

Microbial-type terpene synthases significantly contribute to the terpene profile of glandular trichomes of the fern Dryopteris fragrans (L.)
Chen L, Gao R, Wei G, Luo S, Köllner TG, Xu H, Jiang Y, Zhang C, Chen X, Dickschat JS, Gershenzon J, Li S, Chang Y, Chen F
Plant J. 2025 Mar;121(6):e70079. doi: 10.1111/tpj.70079.

Forensics

Advancements in differentiation between sperm cells and epithelial cells for efficient forensic DNA analysis in sexual assault cases
Dash HR
Int J Legal Med. 2024 Jul 12. doi: 10.1007/s00414-024-03285-1.

Development of a Simple Cell Harvesting Method to Maximise DNA Recovery from Historic Microscope Slides for Sexual Assault Investigations
Hymus CM, Egan SE and Tay JW
Forensic Sci. 2022, 2(4), 795-807

Population Genetic Issues for Forensic DNA Profiles
Rhodes C
NCJRS, Document Number: 304612, Date Received: April 2022, Award Number: 2017-DN-BX-0136

General Information:
http://projects.nfstc.org/tech_transition/lmd/examiners/

Miscellaneous

Comprehensive biobanking strategy with clinical impact at the European Cancer Moonshot Lund Center
Oskolas H, Nogueira FCN, Domont GB, Yu KH, Semenov YR, Sorger P, Steinfelder E, Corps L, Schulz L, Wieslander E, Fenyö D, Kárpáti S, Holló P, Kemény LV, Döme B, Megyesfalvi Z, Pawłowski K, Nishimura T, Kwon H, Encarnación-Guevara S, Szasz AM, Veréb Z, Gyulai R, Németh IB, Appelqvist R, Rezeli M, Baldetorp B, Horvatovich P, Malmström J, Pla I, Sanchez A, Knudsen B, Kiss A, Malm J, Marko-Varga G, Gil J
J Proteomics. 2025 Apr 15:105442. doi: 10.1016/j.jprot.2025.105442.

Topographic imaging with automatic z-axis correction of Brassica oleracea var. viridis leaves by IR-MALDESI mass spectrometry imaging
Mills Q, Ashbacher SM, Sohn AL, Muddiman DC
Anal Bioanal Chem. 2025 Mar 15. doi: 10.1007/s00216-025-05820-4.

Three-dimensional mass spectrometry imaging (3D MSI): incorporating top-hat IR-MALDESI and automatic z-axis correction
Sohn AL, Witherspoon JG, Smart RC, Muddiman DC
Anal Bioanal Chem. 2025 Mar;417(8):1649-1661. doi: 10.1007/s00216-025-05755-w. 

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Highlight:
Multiple: Laser Microdissection Protocols for Tissue and Cell Isolation
Bio-protocol Selections | 2024
https://en.bio-protocol.org/ebookinfo.aspx?id=23

Live Cell Culture

A 3‑Dimensional Scaffolding System Recapitulates the Hierarchical Osteon Structure
Li X, Sun Y, Wang S, Si C, Li H, and Chang B
ACS Omega 2024, XXXX, XXX, XXX-XXX

A novel method for the isolation of single cells mimicking circulating tumour cells adhered on Smart Bio Surface slides by Laser Capture Microdissection
Visci G, Tolomeo D, Lonoce A, Arshadi A, Bascetta L, Trotta G, van Riel M, Vermeesch JR, Carbone R, Storlazzi CT
Protocols.io, 26 Oct 2023

SPARCS, a platform for genome-scale CRISPR screening for spatial cellular phenotypes
Schmacke NA, Mädler SC, Wallmann G, Metousis A, Bérouti M, Harz H, Leonhardt H, Mann M, Horning V
bioRxiv, 1 June 2023, DOI: 10.1101/2023.06.01.542416