Leica LMD6 & LMD7 레이저 미세절단 현미경 시스템

This curated list features recent scientific publications that have employed Leica’s LMD6 and LMD7 laser microdissection systems in key areas such as cancer research, neuroscience, molecular pathology, forensics, and spatial omics. It is updated regularly to reflect the latest scientific publications utilizing laser microdissection applications.

Laser microdissection (LMD) is an advanced, highly precise sample preparation technique that enables researchers to isolate pure, defined regions of interest, including single cells, from complex tissue samples. The method ensures reproducibility and accuracy for downstream analyses such as Next Generation Sequencing (NGS) including RNA-seq, and mass spectrometry for spatial genomics, spatial transcriptomics, spatial proteomics & spatial multiomics.

LMD allows non-contact dissection, isolation and gravity-assisted collection of targeted single cells, tissue compartments, cell clusters and even subcellular structures under visual control. These systems uniquely bridge microscopy and molecular biology to unlock true spatial omics insights. Combined with software-guided segmentation, Leica LMD systems enable high throughput workflows for biomarker discovery and identification of therapeutical interaction points. The study by Mund & Coscia et al., describing the Deep Visual Proteomics (DVP) workflow powered by the Leica LMD7 system, was featured on the cover of Nature Biotechnology in August 2022.  Follow-up research by Nordmann et al., published in Nature in 2024, demonstrated how DVP contributed to a life-saving therapy for toxic epidermal necrolysis (TEN), a deadly skin disease. These breakthroughs also contributed to the selection of “spatial proteomics” as Nature’s Method of the Year 2024.

Explore the full list below to see how Leica LMD systems are enabling scientific success across the globe.

Download the complete list of LMD publications in "Downloads"

Single Cell Applications

Clinical application of single-cell RNA sequencing in disease and therapy
Nadukkandy AS, Kalaiselvan S, Lin L, Luo Y
Clin Transl Med. 2025 Nov;15(11):e70512. doi: 10.1002/ctm2.70512.

Neuronal gene profiling of tau oligomer-bearing cholinergic nucleus basalis neurons during the onset of Alzheimer's disease
Kara B, Beck JS, Fu Z, Hickey SL, Kanaan NM, Mufson EJ, Ginsberg SD, Counts SE
Acta Neuropathol Commun. 2025 Oct 27;13(1):218. doi: 10.1186/s40478-025-02080-2.

Characterisation of Fibrinogen Deposition and its Associations with Neuronal Loss and Microglial Alterations in the Multiple Sclerosis Motor Cortex
Crane MG
Thesis, 2025

DNA Research

Somatic Cancer Driver Mutation Analysis in Endometriosis with Tumor-Like Presentations
Chen L, Severino E, Wu D-S, Segars J and Shih I-M
PrePrints.org, 9 Oct 2025

Genome-Wide Methylation Pro les of Primary and Matched Distant Metastasis: Insights from the Dutch Early-Stage Melanoma (D-Esmel) Study
Ouwerkerk J, Kerkour T, Mooyaart A, Boers R, Boers J, Gribnau J, Wakkee M, Li Y, Hollestein L
Reserach Square, Oct 8th, 2025

A subset of serous tubal intraepithelial carcinoma (STIC)-like lesions and concurrent high-grade endometrial carcinoma are genomically related entities
da Silva EM, Cruz Paula AD, Dessources K, Gordhandas S, Selenica P, Abu-Rustum NR, Weigelt B, Murali R
Mod Pathol. 2025 Sep 12:100890. doi: 10.1016/j.modpat.2025.100890.

RNA Research

Deletion of cardiac fibroblast growth factor-23 beneficially impacts myocardial energy metabolism in left ventricular hypertrophy
Latic N, Lari A, Sun N, Zupcic A, Oubounyt M, Falivene J, Buck A, Hofer M, Chang W, Kuebler WM, Baumbach J, Walch AK, Grabner A, Erben RG
NPJ Metab Health Dis. 2025 Oct 28;3(1):42. doi: 10.1038/s44324-025-00087-w.

Laser microdissection combined with RNAseq reveals glomerulus-specific transcriptome profiles to discriminate class IV from class V lupus nephritis
Zwiers PJ, Querin V, Kiers J, Mecking M, Hackl M, Molema G and Bajema I
Nephrology Dialysis Transplantation, Volume 40, Issue Supplement_3, October 2025, gfaf116.1127

MMPs and NETs are detrimental in CNS-tuberculosis with MMP Inhibition in CNS-tuberculosis mice improving survival
Poh XY, Loh FK, Bai C, Chong HT, Teo WK, Wong YH, Hong JM, Miow QH, Thong PM, Vilaysane B, Hu TH, Chhabra S, Wang Y, Tiong SC, Fong SM, Kamihigashi M, Rajarethinam R, Looi WD, Cheow ESH, Bonney GK, Pakkiri LS, Drum CL, Peng YF, Lee M, Tan CL, Ding CSL, Lim TCC, Yeo TW, Tay JK, Vallejo AF, Ong CWM
J Neuroinflammation. 2025 Oct 11;22(1):230. doi: 10.1186/s12974-025-03548-7.

Proteomics, Lipidomics, Metabolomics

Single-cell and spatial omics reveal progressive loss of xylem developmental complexity across seed plants
Shuai P, Hsieh JA, Kao CT, Hu CW, Wang R, Kuo SC, Yen MR, Liou PC, Ho YC, Chu CC, Huang S, Liu J, Zhang L, Wu CC, Luo YJ, Li Q, Hsu CC, Huang CL, Su JC, Tseng MC, Chen YL, Mai TL, Lin YJ
Plant Cell. 2025 Oct 19:koaf253. doi: 10.1093/plcell/koaf253.

Identifying the quantity profiles of amyloid signature proteins in different types of renal amyloidosis
Wang S, Li D, Zhang X, Zhou F, Wang S
BMC Nephrol. 2025 Oct 16;26(1):576. doi: 10.1186/s12882-025-04477-6.

Spatial multiomics of acute myocardial infarction reveals immune cell infiltration through the endocardium
Wünnemann F, Sicklinger F, Bestak K, Nimo J, Thiemann T, Amrute JM, Nordbeck M, Hartmann N, Ibarra-Arellano MA, Tanevski J, Chazotte M, Heine C, Frey N, Lavine KJ, Coscia F, Saez-Rodriguez J, Leuschner F, Schapiro D
Nat Cardiovasc Res. 2025 Oct 3. doi: 10.1038/s44161-025-00717-y.

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Highlight 2025: 

Rosenberger FA, et al.
Nature (2025 Apr 16)
https://www.nature.com/articles/s41586-025-08885-4

Making space for spatial biology in the clinic
Nat Biotechnol (6 Jan2025).
https://doi.org/10.1038/s41587-024-02543-2

Highlights 2024: 

Method of the Year 2024: spatial proteomics. 
Nat Methods 21, 2195–2196 (2024). 
https://doi.org/10.1038/s41592-024-02565-3
Nordmann et al.
https://www.nature.com/articles/s41586-024-08061-0

Highlights 2023: scDVP applied
Nordmann et al.
https://doi.org/10.1101/2023.11.11.23295492
Schweizer et al.
https://doi.org/10.1101/2023.11.13.23298409

Highlight 2022: Deep Visual Proteomics defines single-cell identity and heterogeneity
https://www.leica-microsystems.com/science-lab/life-science/precise-spatial-proteomic-information-in-tissues/
On single cell level:
Rosenberger FA et al. (scDVP):
https://doi.org/10.1038/s41592-023-02007-6
Original Paper:
Mund et al.
https://www.nature.com/articles/s41587-022-01302-5
Title page Nature Biotechnology, August 2022
https://www.nature.com/nbt/volumes/40/issues/8

Deep Visual Proteomics (DVP) related

TileDVP: Decoding the Tissue Proteome from H&E Images
Mathian É, Oldenburg L, Chelebian E, Schweizer L, Zonderland G, Egebjerg K, Ummat I, Mund A, Strauss 
In: Erdt, M., et al. Clinical Image-Based Procedures. CLIP 2025. Lecture Notes in Computer Science, vol 16126. Springer, Cham.

Ultrasensitive proteomics uncovers nociceptor diversity and novel pain targets
Chakrabarti S, Makhmut A, Mohammadi A, Luo W, Wang L, Lewin GR, Coscia F
bioRxiv, Sept 21, 2025

Deep visual proteomics reveals an in vivo-like phenotype of orthotopically transplanted human colon organoids
Post F, Hausmann A, Kabatnik S, Steigerwald S, Brand A, Clement DL, Skov J, Lõhmussaar K, Larsen HL, Maimets M, Boyce TL, Jez G, Sato T, Steenholdt C, Rosenberger F, Mund A, Nielsen OH, Jensen KB, Mann M
Cell Systems 16, 101396, September 17, 2025

Fluorescence Applications

Identifying the quantity profiles of amyloid signature proteins in different types of renal amyloidosis
Wang S, Li D, Zhang X, Zhou F, Wang S
BMC Nephrol. 2025 Oct 16;26(1):576. doi: 10.1186/s12882-025-04477-6.

Dual Amyloidosis: A Clinicopathologic and Proteomic Analysis of 111 Patients
Dalland JC, Dasari S, Theis JD, Howard MT, Vrana JA, Dao LN, Chiu A, Rech KL, Larson DP, Nasr SH, Grogan M, Dispenzieri A, McPhail ED
Hum Pathol. 2025 Oct 14:105954. doi: 10.1016/j.humpath.2025.105954.

Regionally mapped astrocytic responses to cortical and white matter stroke show differential roles in astrocyte-induced vascular remodeling
Gleichman AJ, Kawaguchi R, Rathbun EM, Sofroniew MV, Carmichael ST
Neuron. 2025 Oct 10:S0896-6273(25)00711-1. doi: 10.1016/j.neuron.2025.09.023.

Cancer Research

Somatic Cancer Driver Mutation Analysis in Endometriosis with Tumor-Like Presentations
Chen L, Severino E, Wu D-S, Segars J and Shih I-M
PrePrints.org, 9 Oct 2025

Genome-Wide Methylation Pro les of Primary and Matched Distant Metastasis: Insights from the Dutch Early-Stage Melanoma (D-Esmel) Study
Ouwerkerk J, Kerkour T, Mooyaart A, Boers R, Boers J, Gribnau J, Wakkee M, Li Y, Hollestein L
Reserach Square, Oct 8th, 2025

A histone-centric multi-omics study shows that increased H3K4 methylation sustains triple-negative breast cancer phenotypes
Noberini R, Robusti G, Vai A, Savoia EO, Jodice MG, Bertalot G, Çat B, Pallavicini I, Bonizzi G, Capra M, Sangalli CA, Zambelli F, Fusco N, Pece S, Pavesi G, Minucci S, Bonaldi T
Nat Commun. 2025 Sep 30;16(1):8716. doi: 10.1038/s41467-025-63745-z.

Neurosciences

Neuronal gene profiling of tau oligomer-bearing cholinergic nucleus basalis neurons during the onset of Alzheimer's disease
Kara B, Beck JS, Fu Z, Hickey SL, Kanaan NM, Mufson EJ, Ginsberg SD, Counts SE
Acta Neuropathol Commun. 2025 Oct 27;13(1):218. doi: 10.1186/s40478-025-02080-2.

Regionally mapped astrocytic responses to cortical and white matter stroke show differential roles in astrocyte-induced vascular remodeling
Gleichman AJ, Kawaguchi R, Rathbun EM, Sofroniew MV, Carmichael ST
Neuron. 2025 Oct 10:S0896-6273(25)00711-1. doi: 10.1016/j.neuron.2025.09.023.

A bottom-up septal inhibitory circuit mediates anticipatory control of drinking
Xu L, Sun Y, Huang C, Zheng Y, Chen J, Ma X, Shi Q, Liu M, Qiu X, Zhao Q, Gao C, Liao J, Wang Y, Chen Z
Nat Neurosci. 2025 Sep 22. doi: 10.1038/s41593-025-02056-4. Online ahead of print.

Clinical/Pathology/Immunology Research Applications

Clinical application of single-cell RNA sequencing in disease and therapy
Nadukkandy AS, Kalaiselvan S, Lin L, Luo Y
Clin Transl Med. 2025 Nov;15(11):e70512. doi: 10.1002/ctm2.70512.

USP15-dependent modulation of TGF-beta/Smad2/3 signaling: implications for chondrocyte matrix degradation and autophagy in osteoarthritis
Yuan Z, He J, Zhao Z, Chen G, Li Y, Li X 
Sci Rep. 2025 Oct 31;15(1):38142. doi: 10.1038/s41598-025-21847-0.

Deletion of cardiac fibroblast growth factor-23 beneficially impacts myocardial energy metabolism in left ventricular hypertrophy
Latic N, Lari A, Sun N, Zupcic A, Oubounyt M, Falivene J, Buck A, Hofer M, Chang W, Kuebler WM, Baumbach J, Walch AK, Grabner A, Erben RG
NPJ Metab Health Dis. 2025 Oct 28;3(1):42. doi: 10.1038/s44324-025-00087-w.
 

Developmental Research, Epigenetics & Aging

Single-cell and spatial omics reveal progressive loss of xylem developmental complexity across seed plants
Shuai P, Hsieh JA, Kao CT, Hu CW, Wang R, Kuo SC, Yen MR, Liou PC, Ho YC, Chu CC, Huang S, Liu J, Zhang L, Wu CC, Luo YJ, Li Q, Hsu CC, Huang CL, Su JC, Tseng MC, Chen YL, Mai TL, Lin
Plant Cell. 2025 Oct 19:koaf253. doi: 10.1093/plcell/koaf253.

Maternal food-derived signals oscillate in the fetal suprachiasmatic nucleus before its circadian clock develops
Sládek M, Houdek P, Čajka T, Sumová A
PLoS Biol. 2025 Sep 26;23(9):e3003404. doi: 10.1371/journal.pbio.3003404.

Single-cell and spatial multiomics identifies heterogeneous xylem development driven by mechanical stress in Populus
Hsieh JA, Liou PC, Lin CC, Dai X, Hu CW, Wang IF, Dang JF, Ho YC, Cheng KW, Xu W, Kuo SC, Kao CT, Yang DX, Wang R, Xiao K, Lin JS, Hsu CC, Hsu CC, Mai TL, Cheng Y, Tseng MC, Li W, Chen YL, Lin YJ
Dev Cell. 2025 Jul 21:S1534-5807(25)00436-8. doi: 10.1016/j.devcel.2025.06.038.

Plant Research

Single-cell and spatial omics reveal progressive loss of xylem developmental complexity across seed plants
Shuai P, Hsieh JA, Kao CT, Hu CW, Wang R, Kuo SC, Yen MR, Liou PC, Ho YC, Chu CC, Huang S, Liu J, Zhang L, Wu CC, Luo YJ, Li Q, Hsu CC, Huang CL, Su JC, Tseng MC, Chen YL, Mai TL, Lin YJ
Plant Cell. 2025 Oct 19:koaf253. doi: 10.1093/plcell/koaf253.

A cell isolation method from Ligusticum chuanxiong Hort. suitable for obtaining high-quality RNA for Smart-seq
Li R, Wu M, Chen S, Huang L, Wang C, Yu Z, Huang F, Liu X, Zhu N, Song C, Jiang G, Yin X
Plant Methods. 2025 Aug 10;21(1):109. doi: 10.1186/s13007-025-01425-1.

Plant defense-directed discovery of a natural anti-psoriasis agent targeting GAPDH
Zhou TT, Zheng Y, Zhang MW, Gong LH, Guo K, He XP, Liu YC, Gershenzon J, Liu Y, Li SH
Sci Adv. 2025 Jul 25;11(30):eadw2578. doi: 10.1126/sciadv.adw2578.

Forensics

Advancements in differentiation between sperm cells and epithelial cells for efficient forensic DNA analysis in sexual assault cases
Dash HR
Int J Legal Med. 2024 Jul 12. doi: 10.1007/s00414-024-03285-1.

Development of a Simple Cell Harvesting Method to Maximise DNA Recovery from Historic Microscope Slides for Sexual Assault Investigations
Hymus CM, Egan SE and Tay JW
Forensic Sci. 2022, 2(4), 795-807

Population Genetic Issues for Forensic DNA Profiles
Rhodes C
NCJRS, Document Number: 304612, Date Received: April 2022, Award Number: 2017-DN-BX-0136

General Information:
http://projects.nfstc.org/tech_transition/lmd/examiners/

Miscellaneous

Filter-aided expansion proteomics for the spatial analysis of single cells and organelles in FFPE tissue samples
Dong Z, Wu C, Chen J, Jiang W, Piatkevich KD, Zhu Y, Guo T
Nat Protoc. 2025 Oct 9. doi: 10.1038/s41596-025-01256-3.

TileDVP: Decoding the Tissue Proteome from H&E Images
Mathian É, Oldenburg L, Chelebian E, Schweizer L, Zonderland G, Egebjerg K, Ummat I, Mund A, Strauss MT
In: Erdt, M., et al. Clinical Image-Based Procedures. CLIP 2025. Lecture Notes in Computer Science, vol 16126. Springer, Cham.

Spatial proteomics in the age of artificial intelligence: exploring the impact on cancer research
Hagan J
BioTechniques, 1–5. 2025

Live Cell Culture

Ultrasensitive proteomics uncovers nociceptor diversity and novel pain targets
Chakrabarti S, Makhmut A, Mohammadi A, Luo W, Wang L, Lewin GR, Coscia F
bioRxiv, Sept 21, 2025

A 3‑Dimensional Scaffolding System Recapitulates the Hierarchical Osteon Structure
Li X, Sun Y, Wang S, Si C, Li H, and Chang B
ACS Omega 2024, XXXX, XXX, XXX-XXX

A novel method for the isolation of single cells mimicking circulating tumour cells adhered on Smart Bio Surface slides by Laser Capture Microdissection
Visci G, Tolomeo D, Lonoce A, Arshadi A, Bascetta L, Trotta G, van Riel M, Vermeesch JR, Carbone R, Storlazzi CT
Protocols.io, 26 Oct 2023