Leica LMD6 & LMD7 레이저 미세절단 현미경 시스템

This list presents current publications in the major application fields for laser microdissection and is updated monthly.

Laser microdissection is a relatively new technique, having first been developed in the 1990s. It makes it possible to obtain homogenous, ultrapure samples from heterogenous starting material. A researcher can selectively and routinely analyze specific regions of interest, down to single cells, to obtain results that are reproducible. Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected via software, excised from the surrounding tissue by a laser, and collected by gravity into specialized devices for analysis.

The article by Mund et al. on Deep Visual Proteomics makes the cover of Nature Biotechnology for the month of August 2022.

Download the complete list of LMD publications in "Downloads"

Single Cell Applications

OpenDVP: An experimental and computational framework for community-empowered deep visual proteomics
Nimo J, Fritzsche S, Valdes DS, Trinh M, Pentimalli T, Schallenberg S, Klauschen F, Herse F, Florian S, Rajewski N, and Coscia F
bioRxiv preprint doi: doi.org/10.1101/2025.07.13.662099; July 16, 2025.

Deep visual proteomics reveals an in vivo-like phenotype of orthotopically transplanted human colon organoids
Post F, Hausmann A, Kabatnik S, Steigerwald S, Brand A, Clement DL, Skov J, Lõhmussaar K, Larsen HL, Maimets M, Boyce TL, Jez G, Sato T, Steenholdt C, Rosenberger F, Mund A, Nielsen OH, Jensen KB, Mann M
Cell Systems 16, 101396, September 17, 2025

Integration of cell-type resolved spatial proteomics and transcriptomics reveals novel mechanisms in early ovarian cancer
Metousis A, Kenny HA, Shimizu A, Schweizer L. Ben-Moshe S, Bilecz A, Krishnan R, Zhang J, Alcazar I, Kelliher L, Ravi M, Samantaray T, Richter S, Li Y, Wang J, Steigerwald S, Theis FJ, Rosenberger FA, Nordmann TM, Yamada SD, Lastra R, Mann M, and Lengyel E
medRxiv, 28 Aug 2025

DNA Research

A subset of serous tubal intraepithelial carcinoma (STIC)-like lesions and concurrent high-grade endometrial carcinoma are genomically related entities
da Silva EM, Cruz Paula AD, Dessources K, Gordhandas S, Selenica P, Abu-Rustum NR, Weigelt B, Murali R
Mod Pathol. 2025 Sep 12:100890. doi: 10.1016/j.modpat.2025.100890.

A novel m.14677 T > C variant in mitochondrial tRNAGlu gene causes chronic progressive external ophthalmoplegia
Ueda NK, Mimaki M, Ito S, Murakami A, Yokoi S, Nishino I, Katsuno M, Goto YI
J Hum Genet. 2025 Aug 6. doi: 10.1038/s10038-025-01381-7.

Origin of Endometrioid Carcinoma Adjacent to Extraovarian Endometriosis: Detailed Molecular Analyses.
Yamagata T, Watanabe K, Yamanoi K, Kakiuchi N, Mizuno R, Taki M, Murakami R, Yamaguchi K, Hamanishi J, Itoh H, Ogawa S, Mandai M
Int J Gynecol Pathol. 2025 Jul 29. doi: 10.1097/PGP.0000000000001128.

RNA Research

Sepsis-associated acute kidney injury in mice and humans elicits heterogeneous renal microvascular microRNA responses
Luxen M, van der Aart TJ, Jongman RM, Zwiers PJ, Moser J, Pultar M, Skalicky S, Diendorfer AB, Bouma HR, Koeze J, Hackl M, Molema G, van Meurs M
Sci Rep. 2025 Oct 2;15(1):34397. doi: 10.1038/s41598-025-17319-0.

Pathology of a Canine Model of Localized Prostate Carcinoma.
Hoggard NK, Berg FM, Szczepaniak MR, Wang X, Kantake N, Ramamurthy G, Gong L, Hostnik ET, Kumar K, Ghosh A, Luo D, Knopp MV, Keller JM, Keller ET, Exner AA, Basilion JP, Tweedle MF, Rosol TJ
Prostate. 2025 Sep 22. doi: 10.1002/pros.70054.

Downregulation of Chromosome 19 miRNA Cluster and the Tumor-Suppressive Role of miR-517a-3p in Choriocarcinoma
Nishiko Y, Yoshida K, Yasui Y, Yokoi A, Kitagawa M, Inami E, Yoshihara M, Mogi K, Nagao Y, Tamauchi S, Yoshikawa N, Nishino K, Yamamoto E, Niimi K, Kajiyama H
Cancer Sci. 2025 Sep 21. doi: 10.1111/cas.70207.

Proteomics, Lipidomics, Metabolomics

Spatial multiomics of acute myocardial infarction reveals immune cell infiltration through the endocardium
Wünnemann F, Sicklinger F, Bestak K, Nimo J, Thiemann T, Amrute JM, Nordbeck M, Hartmann N, Ibarra-Arellano MA, Tanevski J, Chazotte M, Heine C, Frey N, Lavine KJ, Coscia F, Saez-Rodriguez J, Leuschner F, Schapiro D
Nat Cardiovasc Res. 2025 Oct 3. doi: 10.1038/s44161-025-00717-y.

A histone-centric multi-omics study shows that increased H3K4 methylation sustains triple-negative breast cancer phenotypes
Noberini R, Robusti G, Vai A, Savoia EO, Jodice MG, Bertalot G, Çat B, Pallavicini I, Bonizzi G, Capra M, Sangalli CA, Zambelli F, Fusco N, Pece S, Pavesi G, Minucci S, Bonaldi T
Nat Commun. 2025 Sep 30;16(1):8716. doi: 10.1038/s41467-025-63745-z.

Spatial metabolomics strategy reveals heterogeneity of prostate cancer based on multi-platform imaging and laser microdissection-gas chromatography-tandem mass spectrometry
Pu S, Dou P, Xu T, Wei C, Li Y, Zhou L, Wang F, Lu X, Liu X, Xu G
Talanta. 2025 Sep 28;298(Pt A):128921. doi: 10.1016/j.talanta.2025.128921.

Silva pattern-guided proteomics reveals coordinated tumor-stroma remodeling in cervical adenocarcinoma
Drayer SM, Ogata JD, Cronin S, Tumas J, Movahedi-Lankarani S, Abulez T, Conrads KA, Hood BL, Darcy KM, Tarney CM, Bunch KP, Maxwell GL, Conrads TP, Bateman NW, Phippen NT
Gynecol Oncol. 2025 Sep 26;202:66-76. doi: 10.1016/j.ygyno.2025.09.002.

Fibre supplementation alters the gastrointestinal microbiome, the microbial metabolites and indicators of neurodegeneration in a mouse model of Alzheimer´s disease
Böswald LF, Wenderlein J, Bachmann M, Zeyner A, Neuhaus K, Schäfer F, Imhof A, Lahiri S, Gruetzke J, Popper B
Sci Rep. 2025 Sep 24;15(1):32705. doi: 10.1038/s41598-025-20986-8.

---

Highlight 2025: 

Rosenberger FA, et al.
Nature (2025 Apr 16)
https://www.nature.com/articles/s41586-025-08885-4

Making space for spatial biology in the clinic
Nat Biotechnol (6 Jan2025).
https://doi.org/10.1038/s41587-024-02543-2

Highlights 2024: 

Method of the Year 2024: spatial proteomics. 
Nat Methods 21, 2195–2196 (2024). 
https://doi.org/10.1038/s41592-024-02565-3
Nordmann et al.
https://www.nature.com/articles/s41586-024-08061-0

Highlights 2023: scDVP applied
Nordmann et al.
https://doi.org/10.1101/2023.11.11.23295492
Schweizer et al.
https://doi.org/10.1101/2023.11.13.23298409

Highlight 2022: Deep Visual Proteomics defines single-cell identity and heterogeneity
https://www.leica-microsystems.com/science-lab/life-science/precise-spatial-proteomic-information-in-tissues/
On single cell level:
Rosenberger FA et al. (scDVP):
https://doi.org/10.1038/s41592-023-02007-6
Original Paper:
Mund et al.
https://www.nature.com/articles/s41587-022-01302-5
Title page Nature Biotechnology, August 2022
https://www.nature.com/nbt/volumes/40/issues/8

Deep Visual Proteomics (DVP) related

TileDVP: Decoding the Tissue Proteome from H&E Images
Mathian É, Oldenburg L, Chelebian E, Schweizer L, Zonderland G, Egebjerg K, Ummat I, Mund A, Strauss 
In: Erdt, M., et al. Clinical Image-Based Procedures. CLIP 2025. Lecture Notes in Computer Science, vol 16126. Springer, Cham.

Ultrasensitive proteomics uncovers nociceptor diversity and novel pain targets
Chakrabarti S, Makhmut A, Mohammadi A, Luo W, Wang L, Lewin GR, Coscia F
bioRxiv, Sept 21, 2025

Deep visual proteomics reveals an in vivo-like phenotype of orthotopically transplanted human colon organoids
Post F, Hausmann A, Kabatnik S, Steigerwald S, Brand A, Clement DL, Skov J, Lõhmussaar K, Larsen HL, Maimets M, Boyce TL, Jez G, Sato T, Steenholdt C, Rosenberger F, Mund A, Nielsen OH, Jensen KB, Mann M
Cell Systems 16, 101396, September 17, 2025

Fluorescence Applications

A bottom-up septal inhibitory circuit mediates anticipatory control of drinking
Xu L, Sun Y, Huang C, Zheng Y, Chen J, Ma X, Shi Q, Liu M, Qiu X, Zhao Q, Gao C, Liao J, Wang Y, Chen Z
Nat Neurosci. 2025 Sep 22. doi: 10.1038/s41593-025-02056-4. Online ahead of print.

Deep visual proteomics reveals an in vivo-like phenotype of orthotopically transplanted human colon organoids
Post F, Hausmann A, Kabatnik S, Steigerwald S, Brand A, Clement DL, Skov J, Lõhmussaar K, Larsen HL, Maimets M, Boyce TL, Jez G, Sato T, Steenholdt C, Rosenberger F, Mund A, Nielsen OH, Jensen KB, Mann M
Cell Systems 16, 101396, September 17, 2025

Temporal transcriptional regulation of mitochondrial morphology primes activity-dependent circuit connectivity
Mohylyak I, Andriatsilavo M, Bengochea M, Pascual-Caro C, Asfogo N, Fonseca-Topp S, Danda N, Cassar M, Marie C, Atak ZK, De Waegeneer M, Aerts S, Corti O, de Juan-Sanz J, Hassan BA
Nat Commun. 2025 Sep 1;16(1):8173. doi: 10.1038/s41467-025-62908-2.

Cancer Research

A histone-centric multi-omics study shows that increased H3K4 methylation sustains triple-negative breast cancer phenotypes
Noberini R, Robusti G, Vai A, Savoia EO, Jodice MG, Bertalot G, Çat B, Pallavicini I, Bonizzi G, Capra M, Sangalli CA, Zambelli F, Fusco N, Pece S, Pavesi G, Minucci S, Bonaldi T
Nat Commun. 2025 Sep 30;16(1):8716. doi: 10.1038/s41467-025-63745-z.

Silva pattern-guided proteomics reveals coordinated tumor-stroma remodeling in cervical adenocarcinoma
Drayer SM, Ogata JD, Cronin S, Tumas J, Movahedi-Lankarani S, Abulez T, Conrads KA, Hood BL, Darcy KM, Tarney CM, Bunch KP, Maxwell GL, Conrads TP, Bateman NW, Phippen NT
Gynecol Oncol. 2025 Sep 26;202:66-76. doi: 10.1016/j.ygyno.2025.09.002.

Pathology of a Canine Model of Localized Prostate Carcinoma.
Hoggard NK, Berg FM, Szczepaniak MR, Wang X, Kantake N, Ramamurthy G, Gong L, Hostnik ET, Kumar K, Ghosh A, Luo D, Knopp MV, Keller JM, Keller ET, Exner AA, Basilion JP, Tweedle MF, Rosol TJ
Prostate. 2025 Sep 22. doi: 10.1002/pros.70054.

Downregulation of Chromosome 19 miRNA Cluster and the Tumor-Suppressive Role of miR-517a-3p in Choriocarcinoma
Nishiko Y, Yoshida K, Yasui Y, Yokoi A, Kitagawa M, Inami E, Yoshihara M, Mogi K, Nagao Y, Tamauchi S, Yoshikawa N, Nishino K, Yamamoto E, Niimi K, Kajiyama H
Cancer Sci. 2025 Sep 21. doi: 10.1111/cas.70207.

Neurosciences

A bottom-up septal inhibitory circuit mediates anticipatory control of drinking
Xu L, Sun Y, Huang C, Zheng Y, Chen J, Ma X, Shi Q, Liu M, Qiu X, Zhao Q, Gao C, Liao J, Wang Y, Chen Z
Nat Neurosci. 2025 Sep 22. doi: 10.1038/s41593-025-02056-4. Online ahead of print.

Hyperactivity of subicular parvalbumin interneurons drives early amyloid pathology and cognitive deficits in Alzheimer's disease
Chen YB, Jiang B, Zhuang K, Wang H, Peng HM, Zhong BW, Xie WT, Chen KW, Zou TT, Wang Y, Yang HL, Yang Q, Zhou J, Zhong L, Zhang LH, Zhang J
Mol Psychiatry. 2025 Sep 6. doi: 10.1038/s41380-025-03217-4.

Temporal transcriptional regulation of mitochondrial morphology primes activity-dependent circuit connectivity
Mohylyak I, Andriatsilavo M, Bengochea M, Pascual-Caro C, Asfogo N, Fonseca-Topp S, Danda N, Cassar M, Marie C, Atak ZK, De Waegeneer M, Aerts S, Corti O, de Juan-Sanz J, Hassan BA
Nat Commun. 2025 Sep 1;16(1):8173. doi: 10.1038/s41467-025-62908-2.

Clinical/Pathology/Immunology Research Applications

Spatial multiomics of acute myocardial infarction reveals immune cell infiltration through the endocardium
Wünnemann F, Sicklinger F, Bestak K, Nimo J, Thiemann T, Amrute JM, Nordbeck M, Hartmann N, Ibarra-Arellano MA, Tanevski J, Chazotte M, Heine C, Frey N, Lavine KJ, Coscia F, Saez-Rodriguez J, Leuschner F, Schapiro D
Nat Cardiovasc Res. 2025 Oct 3. doi: 10.1038/s44161-025-00717-y.

Sepsis-associated acute kidney injury in mice and humans elicits heterogeneous renal microvascular microRNA responses
Luxen M, van der Aart TJ, Jongman RM, Zwiers PJ, Moser J, Pultar M, Skalicky S, Diendorfer AB, Bouma HR, Koeze J, Hackl M, Molema G, van Meurs M
Sci Rep. 2025 Oct 2;15(1):34397. doi: 10.1038/s41598-025-17319-0.

Comprehensive Histopathological and Biochemical Analyzes of Prostatic Amyloid Bodies (Corpora Amylacea) From Autopsy Samples From Japanese Patients
Yatsuda J, Kanenawa K, Nomura T, Okada M, Masuda T, Misumi Y, Tasaki M, Ueda M, Ando Y, Kamba T
Prostate. 2025 Sep 23. doi: 10.1002/pros.70058.

Fibre supplementation alters the gastrointestinal microbiome, the microbial metabolites and indicators of neurodegeneration in a mouse model of Alzheimer´s disease
Böswald LF, Wenderlein J, Bachmann M, Zeyner A, Neuhaus K, Schäfer F, Imhof A, Lahiri S, Gruetzke J, Popper B
Sci Rep. 2025 Sep 24;15(1):32705. doi: 10.1038/s41598-025-20986-8.
 

Developmental Research, Epigenetics & Aging

Maternal food-derived signals oscillate in the fetal suprachiasmatic nucleus before its circadian clock develops
Sládek M, Houdek P, Čajka T, Sumová A
PLoS Biol. 2025 Sep 26;23(9):e3003404. doi: 10.1371/journal.pbio.3003404.

Single-cell and spatial multiomics identifies heterogeneous xylem development driven by mechanical stress in Populus
Hsieh JA, Liou PC, Lin CC, Dai X, Hu CW, Wang IF, Dang JF, Ho YC, Cheng KW, Xu W, Kuo SC, Kao CT, Yang DX, Wang R, Xiao K, Lin JS, Hsu CC, Hsu CC, Mai TL, Cheng Y, Tseng MC, Li W, Chen YL, Lin YJ
Dev Cell. 2025 Jul 21:S1534-5807(25)00436-8. doi: 10.1016/j.devcel.2025.06.038.

A new paradigm of islet adaptations in human pregnancy: insights from immunohistochemistry and proteomics.
Seedat F, Holden K, Davis S, Fischer R, Bancroft J, Drydale E, Kandzija N, Todd JA, Vatish M, Stefana MI
Nat Commun. 2025 Jul 21;16(1):6687. doi: 10.1038/s41467-025-61852-5.

Plant Research

A cell isolation method from Ligusticum chuanxiong Hort. suitable for obtaining high-quality RNA for Smart-seq
Li R, Wu M, Chen S, Huang L, Wang C, Yu Z, Huang F, Liu X, Zhu N, Song C, Jiang G, Yin X
Plant Methods. 2025 Aug 10;21(1):109. doi: 10.1186/s13007-025-01425-1.

Plant defense-directed discovery of a natural anti-psoriasis agent targeting GAPDH
Zhou TT, Zheng Y, Zhang MW, Gong LH, Guo K, He XP, Liu YC, Gershenzon J, Liu Y, Li SH
Sci Adv. 2025 Jul 25;11(30):eadw2578. doi: 10.1126/sciadv.adw2578.

Comprehensive analysis of imprinted genes in citrus endosperm and their contributions to seed development
He JJ, Hu G, Shen MY, Fan YJ, Shi XS, Wu XM, Guo WW, Xia QM, Xie KD
Plant J. 2025 Jun;122(6):e70290. doi: 10.1111/tpj.70290.

Forensics

Advancements in differentiation between sperm cells and epithelial cells for efficient forensic DNA analysis in sexual assault cases
Dash HR
Int J Legal Med. 2024 Jul 12. doi: 10.1007/s00414-024-03285-1.

Development of a Simple Cell Harvesting Method to Maximise DNA Recovery from Historic Microscope Slides for Sexual Assault Investigations
Hymus CM, Egan SE and Tay JW
Forensic Sci. 2022, 2(4), 795-807

Population Genetic Issues for Forensic DNA Profiles
Rhodes C
NCJRS, Document Number: 304612, Date Received: April 2022, Award Number: 2017-DN-BX-0136

General Information:
http://projects.nfstc.org/tech_transition/lmd/examiners/

Miscellaneous

TileDVP: Decoding the Tissue Proteome from H&E Images
Mathian É, Oldenburg L, Chelebian E, Schweizer L, Zonderland G, Egebjerg K, Ummat I, Mund A, Strauss MT
In: Erdt, M., et al. Clinical Image-Based Procedures. CLIP 2025. Lecture Notes in Computer Science, vol 16126. Springer, Cham.

Spatial proteomics in the age of artificial intelligence: exploring the impact on cancer research
Hagan J
BioTechniques, 1–5. 2025

Single-cell multi-omics in cancer immunotherapy: from tumor heterogeneity to personalized precision treatment
Le J, Dian Y, Zhao D, Guo Z, Luo Z, Chen X, Zeng F, Deng G
Mol Cancer. 2025 Aug 25;24(1):221. doi: 10.1186/s12943-025-02426-3.

Live Cell Culture

Ultrasensitive proteomics uncovers nociceptor diversity and novel pain targets
Chakrabarti S, Makhmut A, Mohammadi A, Luo W, Wang L, Lewin GR, Coscia F
bioRxiv, Sept 21, 2025

A 3‑Dimensional Scaffolding System Recapitulates the Hierarchical Osteon Structure
Li X, Sun Y, Wang S, Si C, Li H, and Chang B
ACS Omega 2024, XXXX, XXX, XXX-XXX

A novel method for the isolation of single cells mimicking circulating tumour cells adhered on Smart Bio Surface slides by Laser Capture Microdissection
Visci G, Tolomeo D, Lonoce A, Arshadi A, Bascetta L, Trotta G, van Riel M, Vermeesch JR, Carbone R, Storlazzi CT
Protocols.io, 26 Oct 2023