Overview
Fluorescence Lifetime Imaging Microscopy-
Analytical Extension of Leica TCS SP2 AOBS Confocal System
FLIM is a technique to measure ion concentrations, intracellular signal transduction, FRET (Fluorescence Resonant Energy Transfer), membrane potential and more using time-correlated single photon counting technology. Striking advantages of this method are its insensitivity to shading, to photo-bleaching, concentration fluctuations of the fluorochrom itself, to excitation intensity, and to light source noise.
The D FLIM2 extension uses a pulsed 405 nm diode laser as excitation source and time reference. The detection system is coupled to the external port of the system, not restricting the use of the basic instrument for confocal imaging.
Key Features
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Usage of 405 nm pulsed diode laser as excitation source
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Advantage: affordable FLIM solution
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405nm pulsed diode laser also provides UV imaging capability
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Variable laser pulse frequency for complete experimental control and flexibility
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Easier to use and maintain
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Usage of a wide range of fluorochromes and fluorescent proteins
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Sophisticated design minimizes effect of incident light thus enhancing signal to noise ratio
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Full Spectral Confocal with four fluorescence detector channels for regular intensity imaging
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Usage of the pulsed diode laser as excitation source for regular intensity imaging
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Retrofit of Leica TCS SP2 AOBS systems
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Data analysis on independent workstation keeps the Confocal free
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Fluorescence decay data for each single pixel
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Single or multiple exponential decay analysis
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