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Application Reports
Visual Inspection with the Stereomicroscope
Polarization Set for Fatigue-Free Work
Every photographer knows the value of a polarization filter for absorbing disturbing light reflections – whether from water surfaces or shiny metals. But light reflections can be a serious problem in industry, too. For example, reflecting incident light makes it considerably more difficult to...
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Targeting of peroxisomal matrix proteins in the diatom Phaeodactylum tricornutum
P. tricornutum cells expressing different types of GFP fusion proteins were harvested via centrifugation at 1,500xg and cryoimmobilized by high-pressure freezing on gold carriers
Sample Preparation:P. tricornutum cells expressing different types of GFP fusion proteins were harvested via centrifugation at 1,500xg and cryoimmobilized by high-pressure freezing on gold carriers (Leica EM PACT2, Leica Microsystems GmbH, Vienna, Austria). Subsequently, the cells were...
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Sample Preparation of Arabidopsis thaliana root tips cells
by Riet De Rycke, resp. DMBR-PSB Transmission Electron Microscopy-Core Facility. Department for Molecular Biomedical Research, Plant Systems Biology, Gent
This Application Note describes the preparation method "Electron Microscopy of high pressure frozen and freeze substituted Arabidopsis thaliana root tips cells" using Leica EM AFS2.
Arabidopsis thaliana roots (mutant PIN1pro:PIN1-GFP;bex5-1) were excised, immersed in 20% (w/v)...
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High Magnification Sperm inspection (IMSI)
IMSI is an advanced ICSI method descibed by Bartoov et al. (in Journal of Andrology 2002, 23).
Prior to sperm injection the morphology of the sperm cell is evaluated with high magnification DIC microscopy.Leica Microsystems offers an aplication optimized system called the Leica "Big S". This has been developped in close cooperation with the lab of Prof. S. Viville, Strassbourg,...
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White Light Laser – the Ultimate Source for Confocal Microscopy
The perfect light source for confocal microscopes in biomedical applications has sufficient intensity, tunable color and is pulsed for use in lifetime fluorescence.
Furthermore, it should be a pulsed source for lifetime fluorescence experiments. Such a source has been invented: the white light laser
Rolf T. Borlinghaus, Dr. 1 , Lioba Kuschel, Dr. 11Leica Microsystems, Wetzlar, Germany
Read article: White...
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