Overview
With its ultrahigh frame rate and gentle handling of samples, Leica’s
SD6000 Spinning Disk Confocal Unit is ideal for live cell experiments.
Used together with the Leica AF6000 LX Live Cell Station, the advantages of high-resolution confocal microscopy are combined with those of widefield fluorescence in one single system. One simple keystroke switches between confocal image recording, widefield fluorescence, a transmitted light method or even the optional TIRF technique. One and the same highly sensitive fluorescence camera serves as the detector for all these methods.
Key Features
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Multipoint scanning at 1000 Hz for real-time confocal imaging
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Just one highly sensitive EMCCD camera for confocal and widefield techniques
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Minimal fading of fluorescence signals allows longer examinations of living samples
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Fully integrated into the LAS AF user software, making it easy to use and offering a wealth of additional application features
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Quick and easy switching between confocal and widefield fluorescence or transmitted light
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Compatible with the inverted research microscope Leica DMI6000, climate chamber and MultiColor-TIRF Module Leica AM TIRF MC
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Filter wheels enable use of the entire visible spectrum
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The Leica EL6000 offers an easy-maintenance, homogeneous and economical light source
Thanks to the use of highly sensitive fluorescence cameras, samples need only be exposed to low illumination intensities. The Leica EL6000 is used as a metal halogenide white light source. Photobleaching and phototoxicity are thus reduced to a minimum, enabling biological processes in living cells to be observed over long periods of time.
The Leica SD6000 is fully integrated in the LAS AF user software as an extra contrasting method. Offering extreme ease-of-use, the software interface allows experiments to be set up with a few simple clicks. Leica´s spinning disk is perfectly matched to the Leica DMI6000 inverted research microscope and is compatible with the MultiColor-TIRF module Leica AM TIRF MC. Adding a climate chamber will maintain a constant environment
for live cells. The Leica SD6000 spinning disk unit can be retrofitted to all Leica AF6000 LX Live Cell stations.